Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   SGN16_RS24935 Genome accession   NZ_CP146985
Coordinates   5639859..5640269 (-) Length   136 a.a.
NCBI ID   WP_335945957.1    Uniprot ID   -
Organism   Pseudomonas sp. G166     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5634859..5645269
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGN16_RS24920 - 5635689..5638622 (+) 2934 WP_335946024.1 DUF748 domain-containing protein -
  SGN16_RS24925 - 5638624..5638920 (+) 297 WP_210640977.1 DUF2845 domain-containing protein -
  SGN16_RS24930 - 5639236..5639589 (-) 354 WP_003205664.1 BON domain-containing protein -
  SGN16_RS24935 pilA 5639859..5640269 (-) 411 WP_335945957.1 pilin Machinery gene
  SGN16_RS24940 pilB 5640494..5642194 (+) 1701 WP_210640980.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SGN16_RS24945 pilC 5642197..5643414 (+) 1218 WP_210640982.1 type II secretion system F family protein Machinery gene
  SGN16_RS24950 pilD 5643416..5644285 (+) 870 WP_210640984.1 A24 family peptidase Machinery gene
  SGN16_RS24955 coaE 5644297..5644920 (+) 624 WP_210640986.1 dephospho-CoA kinase -
  SGN16_RS24960 yacG 5644917..5645123 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13788.96 Da        Isoelectric Point: 8.4972

>NTDB_id=945347 SGN16_RS24935 WP_335945957.1 5639859..5640269(-) (pilA) [Pseudomonas sp. G166]
MNKHKGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLGEISALKVPYEDMMNQGTNPTAANMNITTPTSNCAIA
VTGTASAGTGTIVCTLSNAPAAVAGKTITLSRDAAGAWTCASNAAKEFLPAGCPGV

Nucleotide


Download         Length: 411 bp        

>NTDB_id=945347 SGN16_RS24935 WP_335945957.1 5639859..5640269(-) (pilA) [Pseudomonas sp. G166]
ATGAATAAGCACAAGGGTTTTACGCTGATCGAGCTGCTGATCGTCGTGGCGATCATCGGCATTCTGGCAACGTTTGCGTT
GCCGCAATATTCCAAGTACCAGGCGCGAGCCAAGGCTACGGCCGGGCTTGGGGAGATTTCTGCGTTGAAGGTACCGTACG
AGGACATGATGAATCAGGGAACTAACCCAACAGCGGCCAACATGAACATCACGACGCCTACCAGCAACTGCGCAATAGCA
GTCACGGGTACTGCTTCGGCAGGCACAGGCACCATCGTTTGTACCCTCTCAAATGCTCCTGCTGCTGTAGCGGGTAAGAC
GATCACCCTTAGCCGAGACGCCGCCGGTGCCTGGACATGCGCATCTAACGCTGCCAAGGAATTCCTGCCGGCTGGCTGCC
CTGGCGTCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-133)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.175

100

0.485
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.118

100

0.441
  comP Acinetobacter baylyi ADP1

38

100

0.419
  pilA Ralstonia pseudosolanacearum GMI1000

36.601

100

0.412
  pilA/pilA1 Eikenella corrodens VA1

37.415

100

0.404
  pilA Vibrio cholerae strain A1552

38.298

100

0.397
  pilA Vibrio cholerae C6706

38.298

100

0.397
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.298

100

0.397
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

99.265

0.397
  pilA2 Legionella pneumophila str. Paris

37.241

100

0.397
  pilA Pseudomonas aeruginosa PAK

36.486

100

0.397
  pilA2 Legionella pneumophila strain ERS1305867

36.552

100

0.39