Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   SGN16_RS24940 Genome accession   NZ_CP146985
Coordinates   5640494..5642194 (+) Length   566 a.a.
NCBI ID   WP_210640980.1    Uniprot ID   -
Organism   Pseudomonas sp. G166     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5635494..5647194
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGN16_RS24920 - 5635689..5638622 (+) 2934 WP_335946024.1 DUF748 domain-containing protein -
  SGN16_RS24925 - 5638624..5638920 (+) 297 WP_210640977.1 DUF2845 domain-containing protein -
  SGN16_RS24930 - 5639236..5639589 (-) 354 WP_003205664.1 BON domain-containing protein -
  SGN16_RS24935 pilA 5639859..5640269 (-) 411 WP_335945957.1 pilin Machinery gene
  SGN16_RS24940 pilB 5640494..5642194 (+) 1701 WP_210640980.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SGN16_RS24945 pilC 5642197..5643414 (+) 1218 WP_210640982.1 type II secretion system F family protein Machinery gene
  SGN16_RS24950 pilD 5643416..5644285 (+) 870 WP_210640984.1 A24 family peptidase Machinery gene
  SGN16_RS24955 coaE 5644297..5644920 (+) 624 WP_210640986.1 dephospho-CoA kinase -
  SGN16_RS24960 yacG 5644917..5645123 (+) 207 WP_003205675.1 DNA gyrase inhibitor YacG -
  SGN16_RS24965 - 5645170..5645385 (-) 216 WP_210640988.1 hypothetical protein -
  SGN16_RS24970 - 5645449..5646135 (-) 687 WP_210640990.1 energy-coupling factor ABC transporter permease -
  SGN16_RS24975 - 5646210..5646623 (-) 414 WP_210640992.1 tautomerase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62819.81 Da        Isoelectric Point: 5.5407

>NTDB_id=945348 SGN16_RS24940 WP_210640980.1 5640494..5642194(+) (pilB) [Pseudomonas sp. G166]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQHSRTPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDEHKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLATRIAARL
KVMASLDISERRKPQDGRLKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKRTIEVPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGMTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=945348 SGN16_RS24940 WP_210640980.1 5640494..5642194(+) (pilB) [Pseudomonas sp. G166]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAACTGCTCACCGAGCAAAGTGCGCAGCAGGC
GTACCAACAGGCCCAGCACAGTCGCACGCCCCTGGTCAGCTATCTGGTACAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCATTTTGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAAGAGACCCAACCCACGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAGCACCACGCCCTGCCACTGTGGCGGCGTGGCAATAAATTGTTCGTGGGCATCTCCGA
CCCGACCAACCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAGAAGTTCTTCGAATCCAGTAGCACGGGCCTGGAAGGTATGGGCGATGTCGACCTCGATGGC
TTGGACATCGAGTCGATCGACGAGCACAAGCAGGATTCCATCGCTGGCCAGGACACCGACGACGCGCCGGTGGTGCGGTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCCTCCGATTTGCACTTCGAGCCCTATGAAAAAATCTACCGGG
TGCGGGTGCGTACCGATGGCATGTTGCGGGAGGTGGCCCGGCCACCGATCCAGTTGGCCACCCGTATCGCCGCACGCCTC
AAGGTGATGGCCAGCCTCGATATCTCCGAACGGCGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAAGCAA
ATCGATCGATTTCCGGGTCAACACCCTGCCCACCCTCTGGGGCGAAAAAGTGGTGATCCGGATTCTCGACCCGTCCAGCG
CACAAATGGGCATCGATGCCCTGGGCTACGAACCGGATCAGAAAGACCTGTACATGGCGGCCCTCAAACAACCACAAGGG
TTGATCCTGGTGACCGGGCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGGTCTGAATATCCTCAACACCGTGGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCACGCCAGG
GGCTGGATTTCGCTCAAGCCCTACGCTCGTTCCTGCGCCAAGACCCGGATGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCCATCAAGGCAGCGCAGACCGGGCACCTGGTGCTCTCCACGCTGCACACCAACAGCGCCGCCGA
AACCTTGATTCGCCTGCAGAACATGGGCATTCCGGGCTTCAATATCGCCACCGCTGTACACCTGATCATCGCCCAACGGC
TGGCGCGCAAGCTGTGCAACCACTGCAAGAGAACCATCGAGGTGCCTGAGGAGACCCTGCTCAAGGAAGGTTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTTGGATGCGAACAGTGCAACCACGGTTACAAAGGGCGCGTAGGGGT
TTATGAAGTGGTCAAGAACACACCTGAGCTGCAACGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGCACATCGGGGCTGCTCAAGGTGATGCAGGGCATGACCAGCCTCGAGGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.834

100

0.588
  pilB Acinetobacter baumannii D1279779

56.915

99.647

0.567
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.557
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.759

99.647

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

49.455

97.173

0.481
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481