Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V6C41_RS03725 Genome accession   NZ_CP146228
Coordinates   857308..858330 (-) Length   340 a.a.
NCBI ID   WP_342699810.1    Uniprot ID   -
Organism   Dickeya sp. CFBP 1272     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 852308..863330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V6C41_RS03710 (V6C41_03715) - 852652..853623 (+) 972 WP_407273995.1 PucR family transcriptional regulator -
  V6C41_RS03715 (V6C41_03720) codA 853626..854867 (+) 1242 WP_407321749.1 cytosine deaminase -
  V6C41_RS03720 (V6C41_03725) - 855191..857263 (+) 2073 WP_342699809.1 EAL domain-containing protein -
  V6C41_RS03725 (V6C41_03730) pilT 857308..858330 (-) 1023 WP_342699810.1 type IV pilus twitching motility protein PilT Machinery gene
  V6C41_RS03730 (V6C41_03735) - 858352..859089 (+) 738 WP_342699811.1 YggS family pyridoxal phosphate-dependent enzyme -
  V6C41_RS03735 (V6C41_03740) proC 859122..859943 (+) 822 WP_407273997.1 pyrroline-5-carboxylate reductase -
  V6C41_RS03740 (V6C41_03745) - 859987..860541 (+) 555 WP_342699813.1 YggT family protein -
  V6C41_RS03745 (V6C41_03750) yggU 860538..860828 (+) 291 WP_012886029.1 DUF167 family protein YggU -
  V6C41_RS03750 (V6C41_03755) - 860876..861469 (+) 594 WP_342699814.1 XTP/dITP diphosphatase -
  V6C41_RS03755 (V6C41_03760) hemW 861462..862601 (+) 1140 WP_342699815.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36985.20 Da        Isoelectric Point: 6.4924

>NTDB_id=942214 V6C41_RS03725 WP_342699810.1 857308..858330(-) (pilT) [Dickeya sp. CFBP 1272]
MDVSEWVNASVKQNVSDLHLCSGHPPVRRIDGQLVADATQPPLHHTELEQWCQSWLDPECEQQLRRCGQTDCALTLAGGQ
RLRANLFMQRHGLSAALRLIPHCQPDLQALRAPAVVAALAERTDGLILVTGATGSGKSTTLAAMIAALNQRCARHIITLE
DPIEFLHTSQRSLIQQREIGRHSEGFASALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRRSVQAVER
LVDVFPAEEKAFVRTQLAASLQAVVAQKLLPGVQGGRVALFEVLLATPAVSNLIREGKTHQLPSLLQTGSQVGMQTFEQS
YQQRCQDGLLADGCADDNGL

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=942214 V6C41_RS03725 WP_342699810.1 857308..858330(-) (pilT) [Dickeya sp. CFBP 1272]
ATGGATGTGAGTGAATGGGTGAACGCTAGTGTAAAACAGAATGTATCCGATCTGCACCTGTGCAGCGGTCATCCTCCGGT
GCGGCGTATCGACGGCCAACTGGTGGCGGATGCTACCCAGCCGCCATTACATCACACCGAACTGGAACAGTGGTGCCAGA
GCTGGCTGGACCCGGAGTGTGAACAGCAGTTGCGCCGGTGCGGGCAGACCGACTGTGCGTTGACGCTGGCAGGAGGGCAG
CGGTTGCGGGCCAATCTGTTTATGCAGCGCCACGGTTTATCGGCGGCGCTACGCCTGATCCCACACTGCCAGCCGGATTT
GCAGGCACTGAGGGCACCCGCGGTCGTCGCGGCATTGGCTGAACGTACGGATGGACTGATTCTAGTGACCGGGGCGACCG
GCAGCGGGAAATCGACCACCCTGGCGGCGATGATCGCCGCACTGAATCAACGCTGTGCCCGCCATATTATTACGCTGGAA
GACCCGATTGAATTCCTGCATACCAGTCAACGTAGCCTGATCCAGCAGCGGGAAATCGGCCGACACAGTGAGGGGTTCGC
CAGCGCCTTACGGGCTGCGTTGCGTGAAGACCCGGATGTGTTGTTATTGGGGGAGTTGCGTGACAGCGAGACCATTCGGC
TGGCGCTGACGGCGGCGGAAACCGGTCATCTGGTGCTGGCGACGCTGCATACCCGGCGGTCGGTGCAGGCGGTGGAGCGA
TTGGTGGACGTGTTCCCGGCCGAAGAGAAAGCCTTTGTGCGCACACAACTGGCGGCGAGTTTGCAGGCGGTAGTGGCACA
GAAGCTCTTGCCGGGCGTGCAGGGTGGGCGTGTGGCACTATTTGAGGTACTGCTTGCCACACCGGCGGTTAGCAATCTGA
TCCGCGAAGGTAAAACCCACCAGTTGCCGAGTTTGTTGCAAACCGGCAGCCAGGTGGGGATGCAGACCTTTGAGCAGAGC
TACCAACAGCGGTGTCAGGACGGCCTGTTAGCGGACGGCTGCGCTGACGATAACGGGCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.107

98.824

0.485
  pilT Vibrio cholerae strain A1552

49.107

98.824

0.485
  pilT Pseudomonas stutzeri DSM 10701

48.348

97.941

0.474
  pilT Neisseria meningitidis 8013

47.619

98.824

0.471
  pilT Pseudomonas aeruginosa PAK

47.748

97.941

0.468
  pilT Neisseria gonorrhoeae MS11

47.321

98.824

0.468
  pilT Legionella pneumophila strain ERS1305867

45.588

100

0.456
  pilT Legionella pneumophila strain Lp02

45.588

100

0.456
  pilT Acinetobacter baylyi ADP1

46.667

97.059

0.453
  pilT Acinetobacter baumannii D1279779

46.108

98.235

0.453
  pilT Acinetobacter baumannii strain A118

46.108

98.235

0.453
  pilT Acinetobacter nosocomialis M2

45.619

97.353

0.444
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.887

93.824

0.412
  pilU Vibrio cholerae strain A1552

37.538

97.941

0.368