Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   D5261_RS04580 Genome accession   NZ_AP025739
Coordinates   1069070..1070284 (+) Length   404 a.a.
NCBI ID   WP_119320897.1    Uniprot ID   A0A402CU38
Organism   Capsulimonas corticalis strain AX-7     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1064070..1075284
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D5261_RS04555 (CCAX7_008860) - 1064758..1064994 (+) 237 WP_119320892.1 hypothetical protein -
  D5261_RS04560 (CCAX7_008870) - 1065360..1066202 (+) 843 WP_119320893.1 DUF1559 domain-containing protein -
  D5261_RS04565 (CCAX7_008880) - 1067053..1068234 (+) 1182 WP_119320894.1 GntR family transcriptional regulator -
  D5261_RS04570 (CCAX7_008890) - 1068247..1068657 (-) 411 WP_165864111.1 type II toxin-antitoxin system VapC family toxin -
  D5261_RS04575 (CCAX7_008900) - 1068654..1068893 (-) 240 WP_119320896.1 type II toxin-antitoxin system prevent-host-death family antitoxin -
  D5261_RS04580 (CCAX7_008910) pilT 1069070..1070284 (+) 1215 WP_119320897.1 type IV pilus twitching motility protein PilT Machinery gene
  D5261_RS04585 (CCAX7_008920) - 1070313..1070693 (+) 381 WP_119320898.1 hypothetical protein -
  D5261_RS04590 (CCAX7_008930) glpK 1070736..1072253 (-) 1518 WP_119320899.1 glycerol kinase GlpK -
  D5261_RS04595 (CCAX7_008940) - 1072298..1073143 (-) 846 WP_218025561.1 MIP family channel protein -
  D5261_RS04600 (CCAX7_008950) - 1073193..1074995 (-) 1803 WP_119320901.1 glycerol-3-phosphate dehydrogenase/oxidase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 43780.55 Da        Isoelectric Point: 6.0155

>NTDB_id=93837 D5261_RS04580 WP_119320897.1 1069070..1070284(+) (pilT) [Capsulimonas corticalis strain AX-7]
MDLEELLKTTVAHKASDLHLTVGAPPAFRLHGEMERLPYPDMTRDVLRAMLLSILTGEQQNKFEEKRDLDFSIEIPGVAR
FRANFFVGRRGESAVFRVIPAEIKSVEDLGLPPVVRELARQEKGLVLVTGPTGSGKSTTLAAMVDMINQERAAHILTVED
PIEFVHQHKKCLVNQREVKVHTETFASALRAALREDPDVILVGEMRDLETISLAVSAAETGHLVFGTLHTSSAAQTVDRI
IDVFPPHQQAQIRTQLAESIQGVIAQTLLPKVGGGRIAALEIMTSTAAVKNLIREGKTFQLPSIIETNTNLGMQTLDQAL
QALLLSGQITPAAAISKGMPPALIATLRLPTLPSEPAYAPAPAPEPPRLILTDVAPAPRAPIQTLADDDLGDLKPIEGNR
AFGR

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=93837 D5261_RS04580 WP_119320897.1 1069070..1070284(+) (pilT) [Capsulimonas corticalis strain AX-7]
ATGGACCTCGAAGAACTTTTGAAAACGACGGTCGCGCATAAGGCGTCCGATCTGCACCTCACCGTCGGCGCGCCGCCGGC
GTTTCGTCTCCATGGCGAGATGGAGCGGCTGCCGTACCCGGATATGACGCGCGATGTTCTGCGCGCCATGCTGCTGAGCA
TTCTTACCGGCGAGCAGCAGAACAAATTTGAGGAAAAGCGCGATCTGGACTTCAGCATCGAAATTCCCGGCGTGGCGCGG
TTTCGGGCGAACTTCTTTGTCGGACGGCGCGGCGAGAGCGCGGTCTTTCGCGTGATCCCGGCGGAGATCAAAAGCGTCGA
GGATTTAGGATTGCCGCCGGTGGTGCGGGAGCTAGCGCGTCAAGAAAAGGGATTGGTGCTGGTGACGGGGCCGACGGGGT
CAGGCAAATCGACGACGCTGGCCGCGATGGTCGATATGATCAACCAGGAGCGAGCCGCGCATATCCTCACGGTGGAGGAT
CCCATTGAGTTCGTGCATCAGCACAAGAAGTGCCTGGTCAATCAGCGCGAAGTGAAGGTGCATACGGAGACATTCGCGAG
CGCCCTGCGCGCCGCGCTGCGGGAAGACCCGGATGTCATCCTGGTCGGCGAGATGCGCGATCTGGAGACGATCTCCCTGG
CCGTCTCGGCGGCGGAGACCGGGCATCTCGTATTCGGCACCCTGCATACCTCCAGCGCCGCGCAGACGGTGGACCGGATC
ATCGATGTCTTCCCGCCCCACCAGCAGGCGCAGATCCGCACGCAGCTCGCCGAATCAATCCAGGGAGTCATCGCGCAGAC
CCTGCTGCCGAAGGTCGGGGGCGGACGCATCGCCGCGCTGGAGATTATGACCAGCACCGCCGCCGTCAAGAACTTGATCC
GCGAGGGCAAGACGTTCCAGCTGCCGTCAATTATTGAGACAAACACCAATCTGGGCATGCAGACTCTGGATCAGGCGCTG
CAAGCGCTGCTGCTCTCCGGACAGATCACTCCGGCCGCCGCCATCTCCAAAGGCATGCCGCCCGCGCTGATCGCCACGCT
TCGGCTGCCCACGCTTCCCAGCGAACCGGCGTACGCGCCGGCCCCCGCGCCGGAGCCGCCCCGGCTGATCCTGACGGATG
TGGCGCCCGCCCCGCGCGCGCCGATCCAGACCTTGGCGGACGACGACCTGGGCGACCTCAAGCCGATTGAGGGAAACCGC
GCCTTCGGCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A402CU38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

57.817

83.911

0.485
  pilT Pseudomonas stutzeri DSM 10701

57.185

84.406

0.483
  pilT Legionella pneumophila strain Lp02

56.686

85.149

0.483
  pilT Legionella pneumophila strain ERS1305867

56.686

85.149

0.483
  pilT Acinetobacter nosocomialis M2

56.598

84.406

0.478
  pilT Acinetobacter baumannii D1279779

56.598

84.406

0.478
  pilT Acinetobacter baumannii strain A118

56.598

84.406

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

56.38

83.416

0.47
  pilT Vibrio cholerae strain A1552

56.38

83.416

0.47
  pilT Pseudomonas aeruginosa PAK

55.718

84.406

0.47
  pilT Neisseria gonorrhoeae MS11

54.03

82.921

0.448
  pilT Neisseria meningitidis 8013

54.03

82.921

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.457

94.307

0.438
  pilU Vibrio cholerae strain A1552

42.941

84.158

0.361

Multiple sequence alignment