Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DPPLL_RS09095 Genome accession   NZ_AP025516
Coordinates   1943563..1944645 (-) Length   360 a.a.
NCBI ID   WP_284154479.1    Uniprot ID   -
Organism   Desulfofustis limnaeus strain PPLL     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1938563..1949645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DPPLL_RS09080 (DPPLL_18150) - 1938899..1939687 (-) 789 WP_284154476.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DPPLL_RS09085 (DPPLL_18160) - 1939745..1941031 (+) 1287 WP_284154477.1 glycosyltransferase N-terminal domain-containing protein -
  DPPLL_RS09090 (DPPLL_18170) - 1941028..1943616 (+) 2589 WP_284154478.1 DNA internalization-related competence protein ComEC/Rec2 -
  DPPLL_RS09095 (DPPLL_18180) pilT 1943563..1944645 (-) 1083 WP_284154479.1 type IV pilus twitching motility protein PilT Machinery gene
  DPPLL_RS09100 (DPPLL_18190) - 1944725..1945315 (-) 591 WP_284154480.1 TIGR00730 family Rossman fold protein -
  DPPLL_RS09105 (DPPLL_18200) - 1945489..1947432 (-) 1944 WP_284154481.1 hypothetical protein -
  DPPLL_RS09110 (DPPLL_18210) selA 1947567..1948976 (-) 1410 WP_284154482.1 L-seryl-tRNA(Sec) selenium transferase -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 40303.98 Da        Isoelectric Point: 7.8143

>NTDB_id=92440 DPPLL_RS09095 WP_284154479.1 1943563..1944645(-) (pilT) [Desulfofustis limnaeus strain PPLL]
MAQIDAFFKLMNDEGASDLHMVADQQPVLRIRGDMERVKFKKMSNDELKAMLYEICPEDKIKLFEETGDVDFGYEIPGLA
RYRCNFFQQKYGIGAVFREIPSEVLTCEQLGLPKVISRLAHLPKGLVLVTGPTGSGKSTTLAAIVDEANKVRKHHILTIE
DPIEFVHKSQKCLINHREIGTHTKGFAQALRGALREDPDIIMVGEMRDLETIALAMEAAMTGHLVFATLHTLNAMKTVDR
VIEIFPATQQGQVRSTLADALKAVVSQTLFKRIDIKGRVAALEILIATPAVRNLIREGKTYQILSAMQTGKKYGMQTMDD
AIMGFLEKKMISPDDAYTNSIEKSKFLKFLRKPPSDFTEV

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=92440 DPPLL_RS09095 WP_284154479.1 1943563..1944645(-) (pilT) [Desulfofustis limnaeus strain PPLL]
ATGGCGCAGATCGATGCATTTTTCAAATTGATGAATGATGAGGGCGCCTCGGACTTGCATATGGTTGCCGACCAGCAACC
GGTGCTGAGAATTCGCGGCGACATGGAGCGCGTCAAGTTCAAGAAGATGAGCAACGACGAGCTCAAAGCCATGCTTTACG
AGATATGCCCGGAGGACAAGATCAAGCTCTTCGAGGAGACAGGCGATGTCGATTTTGGTTACGAGATCCCTGGACTGGCC
CGTTATCGTTGCAATTTTTTTCAGCAGAAGTATGGGATCGGCGCTGTCTTCAGGGAAATCCCCAGTGAGGTCCTGACCTG
CGAACAGCTGGGGTTACCCAAGGTCATTTCCCGTCTCGCCCATCTGCCCAAGGGCTTGGTGTTGGTTACCGGCCCAACCG
GCTCCGGTAAATCGACAACCCTTGCCGCCATCGTCGATGAAGCGAACAAGGTGCGGAAACACCATATCCTGACCATTGAG
GATCCCATCGAGTTTGTGCACAAGAGCCAAAAATGCCTCATTAACCATCGCGAGATCGGTACCCACACCAAAGGGTTCGC
TCAGGCGCTGCGCGGAGCCCTGCGCGAAGATCCCGACATCATCATGGTCGGTGAGATGCGCGACCTGGAAACCATAGCCC
TGGCCATGGAAGCGGCCATGACCGGACACCTGGTTTTCGCCACCCTGCATACGCTCAATGCCATGAAAACGGTGGATCGG
GTGATCGAGATCTTTCCGGCCACCCAGCAAGGCCAGGTCCGCTCCACGTTGGCCGATGCGTTGAAGGCGGTTGTTTCCCA
GACGTTGTTCAAACGGATCGACATTAAAGGGCGGGTGGCGGCGCTGGAAATCCTCATCGCCACGCCGGCCGTGCGAAACC
TGATTCGCGAGGGGAAAACCTATCAGATCCTCTCAGCCATGCAGACCGGCAAAAAATATGGCATGCAGACCATGGACGAT
GCCATCATGGGTTTCCTCGAGAAGAAGATGATCAGCCCCGACGATGCCTATACCAACTCCATAGAGAAATCAAAATTTCT
CAAGTTCCTGAGAAAGCCACCGTCCGATTTCACCGAGGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

55.556

92.5

0.514
  pilT Pseudomonas aeruginosa PAK

56.966

89.722

0.511
  pilT Acinetobacter nosocomialis M2

53.293

92.778

0.494
  pilT Acinetobacter baumannii D1279779

53.293

92.778

0.494
  pilT Acinetobacter baumannii strain A118

53.293

92.778

0.494
  pilT Acinetobacter baylyi ADP1

55.205

88.056

0.486
  pilT Legionella pneumophila strain Lp02

52.266

91.944

0.481
  pilT Legionella pneumophila strain ERS1305867

52.266

91.944

0.481
  pilT Neisseria meningitidis 8013

53.894

89.167

0.481
  pilT Neisseria gonorrhoeae MS11

53.583

89.167

0.478
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.821

96.111

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.787

84.722

0.447
  pilT Vibrio cholerae strain A1552

52.787

84.722

0.447
  pilU Pseudomonas stutzeri DSM 10701

37.853

98.333

0.372

Multiple sequence alignment