Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OCV44_RS02205 Genome accession   NZ_AP025510
Coordinates   449875..450984 (+) Length   369 a.a.
NCBI ID   WP_041472921.1    Uniprot ID   A0A1C3IVG6
Organism   Vibrio tasmaniensis strain LMG 20012     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 451318..482327 449875..450984 flank 334


Gene organization within MGE regions


Location: 449875..482327
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OCV44_RS02205 pilU 449875..450984 (+) 1110 WP_041472921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OCV44_RS02210 ruvX 451318..451740 (-) 423 WP_139686005.1 Holliday junction resolvase RuvX -
  OCV44_RS02215 - 451790..452353 (-) 564 WP_102340779.1 YqgE/AlgH family protein -
  OCV44_RS02220 gshB 452470..453420 (-) 951 WP_017077777.1 glutathione synthase -
  OCV44_RS02225 rsmE 453430..454161 (-) 732 WP_139686004.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OCV44_RS02230 - 454321..455052 (-) 732 WP_139686003.1 endonuclease -
  OCV44_RS02235 - 455150..455650 (-) 501 WP_012604825.1 SprT family zinc-dependent metalloprotease -
  OCV44_RS02240 - 455732..456529 (-) 798 WP_009847692.1 DUF2189 domain-containing protein -
  OCV44_RS02245 - 456811..458115 (-) 1305 WP_139686002.1 cobyrinate a,c-diamide synthase -
  OCV44_RS02250 - 458118..459182 (-) 1065 WP_139686001.1 threonine-phosphate decarboxylase -
  OCV44_RS02255 cbiB 459204..460166 (-) 963 WP_139686000.1 adenosylcobinamide-phosphate synthase CbiB -
  OCV44_RS02260 cobM 460223..461005 (-) 783 WP_139685999.1 precorrin-4 C(11)-methyltransferase -
  OCV44_RS02265 cobJ 461007..461846 (-) 840 WP_139685998.1 precorrin-3B C(17)-methyltransferase -
  OCV44_RS02270 - 461849..462691 (-) 843 WP_139685997.1 sirohydrochlorin cobaltochelatase -
  OCV44_RS02275 - 462937..464541 (-) 1605 WP_139686171.1 cobalamin biosynthesis protein -
  OCV44_RS02280 cobI 464541..465371 (-) 831 WP_139686170.1 precorrin-2 C(20)-methyltransferase -
  OCV44_RS02285 cbiE 465438..466736 (-) 1299 WP_139686169.1 precorrin-6y C5,15-methyltransferase (decarboxylating) subunit CbiE -
  OCV44_RS02290 - 466733..467392 (-) 660 WP_086048717.1 precorrin-8X methylmutase -
  OCV44_RS02295 - 467407..468603 (-) 1197 WP_139686168.1 cobalt-precorrin-5B (C(1))-methyltransferase -
  OCV44_RS02300 - 468839..469303 (-) 465 WP_170213766.1 phosphoglycerate mutase family protein -
  OCV44_RS02305 - 469293..470318 (-) 1026 WP_246091853.1 iron ABC transporter permease -
  OCV44_RS02310 - 470305..471261 (-) 957 WP_139686166.1 ABC transporter substrate-binding protein -
  OCV44_RS02315 - 471258..472016 (-) 759 WP_139686165.1 ABC transporter ATP-binding protein -
  OCV44_RS02320 metK 472352..473503 (-) 1152 WP_009847676.1 methionine adenosyltransferase -
  OCV44_RS02325 tkt 473880..475874 (+) 1995 WP_139685064.1 transketolase -
  OCV44_RS02330 - 476199..476903 (+) 705 WP_139685063.1 response regulator -
  OCV44_RS02335 - 476966..478333 (+) 1368 WP_246091766.1 ATP-binding protein -
  OCV44_RS02340 - 478330..479571 (+) 1242 WP_139685062.1 ABC transporter substrate-binding protein -
  OCV44_RS02345 - 479733..480635 (+) 903 WP_139685061.1 PIG-L family deacetylase -
  OCV44_RS02350 - 480804..482327 (+) 1524 WP_029222971.1 PTS transporter subunit EIIC -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41454.32 Da        Isoelectric Point: 5.6712

>NTDB_id=92352 OCV44_RS02205 WP_041472921.1 449875..450984(+) (pilU) [Vibrio tasmaniensis strain LMG 20012]
MELNQILEGMLSQKASDLYITVDAPILFRVDGELRPQGEKLNADQVSQLLDAMMDQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVVAQQLIRDKNGNGRHGVFEILLNSPRISDLIRRGELHELKATMAKSKEIGMQTFDQALY
DLVVADKISEDDAFHSADSANDLRLMLKTKRGDDDYGTGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=92352 OCV44_RS02205 WP_041472921.1 449875..450984(+) (pilU) [Vibrio tasmaniensis strain LMG 20012]
ATGGAATTGAATCAAATCCTTGAAGGGATGCTCTCTCAAAAGGCGTCGGATCTTTACATCACAGTCGATGCGCCAATCCT
ATTTCGTGTGGATGGTGAACTACGACCTCAGGGAGAGAAGTTGAATGCTGATCAAGTATCTCAGTTACTTGATGCAATGA
TGGATCAAGATCGACGCGATGAATACAAGCAAACGCGTGAGGCTAATTTTGCCATCGTGCGTGATTTTGGTCGTTTTCGT
GTCAGTGCATTCTTTCAGCGAGAGCTACCTGGAGCGGTAATTCGTCGCATCGAGACTAACATCCCAACCTTTGAGCAATT
AAAGCTTCCTGACGTACTACAAGACCTTTCTATCGCTAAGCGCGGACTTGTGCTGGTGGTTGGGGCTACGGGGTCGGGTA
AATCAACCTCGATGGCCGCAATGACAGGCTATCGCAATACCAATCGTTCAGGTCATATCTTGACGGTTGAAGACCCGATT
GAATTCGTACACGAACATAAAAAGTGCATCGTGACTCAACGAGAGGTTGGACTCGACACCGAAAGCTATGAAGTCGCGCT
TAAAAACTCGTTACGCCAAGCCCCTGATATGATTTTGATTGGCGAAATCCGTAGTCGTGAAACCATGGAATACGCGATGA
CCTTTGCTGAAACGGGTCACTTGTGTATGGCAACCCTGCATGCCAATAACGCTAACCAAGCGCTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAGCAGAAAGAGCAGTTCTTGTTTGATCTGTCGATGAATCTGCGTGGTGTGGTTGCTCAGCAGTTAAT
ACGCGATAAAAATGGCAATGGTCGTCATGGTGTCTTCGAAATTCTGCTAAATAGCCCTCGTATATCTGACTTGATTCGTC
GTGGTGAGTTGCATGAGTTAAAAGCGACCATGGCCAAATCGAAAGAGATTGGTATGCAGACTTTTGACCAAGCTTTGTAT
GATTTAGTAGTTGCGGACAAGATTAGCGAGGATGATGCATTCCATAGTGCAGATTCTGCCAATGACTTGCGTTTAATGCT
CAAGACCAAACGTGGCGATGATGACTACGGAACTGGTGCGTTGGCTGGCGTTAAGATTGATATGGGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C3IVG6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.598

99.729

0.854
  pilU Pseudomonas stutzeri DSM 10701

55.795

100

0.561
  pilU Acinetobacter baylyi ADP1

54.416

95.122

0.518
  pilT Legionella pneumophila strain ERS1305867

42.982

92.683

0.398
  pilT Legionella pneumophila strain Lp02

42.982

92.683

0.398
  pilT Acinetobacter nosocomialis M2

41.813

92.683

0.388
  pilT Acinetobacter baumannii strain A118

41.813

92.683

0.388
  pilT Acinetobacter baumannii D1279779

41.813

92.683

0.388
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

91.87

0.385
  pilT Pseudomonas aeruginosa PAK

41.493

90.786

0.377
  pilT Acinetobacter baylyi ADP1

42.638

88.347

0.377
  pilT Pseudomonas stutzeri DSM 10701

40.597

90.786

0.369
  pilT Vibrio cholerae strain A1552

41.538

88.076

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.538

88.076

0.366

Multiple sequence alignment