Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   M3O54_RS17320 Genome accession   NZ_CP142004
Coordinates   4017127..4018260 (+) Length   377 a.a.
NCBI ID   WP_064635139.1    Uniprot ID   A0A3E1KKR3
Organism   Xanthomonas nasturtii strain 8853     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4012127..4023260
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M3O54_RS17295 (M3O54_017270) - 4012275..4013474 (-) 1200 WP_064635146.1 cystathionine gamma-synthase -
  M3O54_RS17300 (M3O54_017275) - 4013576..4014946 (-) 1371 WP_064635249.1 pyridoxal-phosphate dependent enzyme -
  M3O54_RS17305 (M3O54_017280) - 4015185..4015403 (-) 219 WP_005991804.1 YdcH family protein -
  M3O54_RS17310 (M3O54_017285) - 4015757..4016647 (-) 891 WP_064635144.1 hypothetical protein -
  M3O54_RS17315 (M3O54_017290) - 4016644..4017027 (-) 384 WP_064635142.1 DUF4398 domain-containing protein -
  M3O54_RS17320 (M3O54_017295) pilU 4017127..4018260 (+) 1134 WP_064635139.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  M3O54_RS17325 (M3O54_017300) maiA 4018590..4019252 (-) 663 WP_064635137.1 maleylacetoacetate isomerase -
  M3O54_RS17330 (M3O54_017305) - 4019296..4020285 (-) 990 WP_064635135.1 fumarylacetoacetate hydrolase family protein -
  M3O54_RS17335 (M3O54_017310) - 4020662..4022044 (+) 1383 WP_064635133.1 DEAD/DEAH box helicase -
  M3O54_RS17340 (M3O54_017315) queD 4022179..4022532 (+) 354 WP_055850251.1 6-carboxytetrahydropterin synthase QueD -
  M3O54_RS17345 (M3O54_017320) - 4022726..4023061 (+) 336 WP_064635132.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41769.08 Da        Isoelectric Point: 6.1401

>NTDB_id=920851 M3O54_RS17320 WP_064635139.1 4017127..4018260(+) (pilU) [Xanthomonas nasturtii strain 8853]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=920851 M3O54_RS17320 WP_064635139.1 4017127..4018260(+) (pilU) [Xanthomonas nasturtii strain 8853]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCAATTGCCTTGTCCGATGCCGGCCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAATCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGCTCGACCGGCT
CCGGCAAGTCAACTTCGCTGGCATCGATGATCGACCATCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCATGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAAGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCCG
CCATCGCGTTTGCCGAAACCGGTCACCTGTGCCTGGCCACGTTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTCAATCTGCGCGCGGTGGTGTCGCAACG
CCTGGTCAAGGGCGTGGATGGACGCCGCCGGCCGGCGACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGTCAGGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTGAAGTCTGGCGAAATCGAGCAGGAGGAAGCGCTGCGCGCCGCCGATTCACGCGATGGCCTTGCCCTGAA
GTTCCGTCTTTCCGAAGGCGGCTCCGGCGAACACGACCCGTATGCGGACTACGAAACGGCGGCTGCGCCGAAGATCAGCC
ACGGGTTCATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3E1KKR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361