Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   SO574_RS11440 Genome accession   NZ_CP140102
Coordinates   2534223..2535329 (-) Length   368 a.a.
NCBI ID   WP_211915949.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain VA-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2529223..2540329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SO574_RS11410 (SO574_11410) - 2529676..2530173 (+) 498 WP_221068140.1 SprT family zinc-dependent metalloprotease -
  SO574_RS11415 (SO574_11415) endA 2530447..2531142 (+) 696 WP_221068141.1 deoxyribonuclease I -
  SO574_RS11420 (SO574_11420) rsmE 2531278..2532009 (+) 732 WP_322804183.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  SO574_RS11425 (SO574_11425) gshB 2532024..2532974 (+) 951 WP_322803145.1 glutathione synthase -
  SO574_RS11430 (SO574_11430) - 2533114..2533677 (+) 564 WP_221068143.1 YqgE/AlgH family protein -
  SO574_RS11435 (SO574_11435) ruvX 2533708..2534133 (+) 426 WP_322803146.1 Holliday junction resolvase RuvX -
  SO574_RS11440 (SO574_11440) pilU 2534223..2535329 (-) 1107 WP_211915949.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  SO574_RS11445 (SO574_11445) pilT 2535357..2536397 (-) 1041 WP_128810138.1 type IV pilus twitching motility protein PilT Machinery gene
  SO574_RS11450 (SO574_11450) - 2536426..2537136 (+) 711 WP_322803147.1 YggS family pyridoxal phosphate-dependent enzyme -
  SO574_RS11455 (SO574_11455) proC 2537327..2538145 (+) 819 WP_322803148.1 pyrroline-5-carboxylate reductase -
  SO574_RS11460 (SO574_11460) - 2538198..2538755 (+) 558 WP_128810135.1 YggT family protein -
  SO574_RS11465 (SO574_11465) yggU 2538755..2539045 (+) 291 WP_128810134.1 DUF167 family protein YggU -
  SO574_RS11470 (SO574_11470) - 2539109..2539540 (+) 432 WP_211915945.1 DUF4426 domain-containing protein -
  SO574_RS11475 (SO574_11475) - 2539635..2540237 (+) 603 WP_322803149.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41390.39 Da        Isoelectric Point: 6.5076

>NTDB_id=912528 SO574_RS11440 WP_211915949.1 2534223..2535329(-) (pilU) [Vibrio alfacsensis strain VA-1]
MDLNKFLEGMLTLKASDLYITVGAPILFRVDGELRPQGEKLTEHDVAQLLDSAMEPDRRREFRSSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAVAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKAGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPNDQKEQFLFDLSMNLKGVIGQQLIRDKNGQGRHGVFEVLLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGESFSSGSLENVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=912528 SO574_RS11440 WP_211915949.1 2534223..2535329(-) (pilU) [Vibrio alfacsensis strain VA-1]
ATGGATCTGAACAAATTTCTTGAGGGTATGCTCACGCTTAAAGCGTCGGACCTCTATATTACGGTCGGTGCTCCAATACT
GTTTCGAGTTGATGGAGAGCTCCGCCCACAAGGTGAGAAGCTGACCGAGCATGATGTAGCGCAGTTGCTTGATAGTGCCA
TGGAGCCTGATCGTCGCCGAGAATTTCGTAGTAGCCGTGAATCTAACTTCGCTATAGTAAGAGACTGTGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGTGAATTACCAGGGGCGGTGATTCGTCGCATTGAGACCAATATCCCGACCTTTGAGCAATT
AAAGTTGCCGTTGGTTTTGCAAGATCTTGCTGTTGCTAAGCGAGGGCTAGTGCTGGTGGTTGGTGCGACAGGGTCGGGTA
AATCGACGACAATGGCTGCAATGACGGGGTATCGAAACAACAATAAAGCGGGTCATATTCTAACGGTTGAGGATCCCATT
GAGTTTGTGCATGAGCATAAGCGTTGTATCGTCACACAACGAGAAGTGGGTTTAGATACTGAAAGCTACGAGGTGGCGCT
TAAGAACTCGCTCCGTCAAGCGCCAGACATGATTTTGATTGGTGAAATTCGCAGTCGAGAAACGATGGAATATGCGATGA
CGTTCGCGGAAACTGGGCATCTTTGCATGGCAACCCTGCACGCTAACAATGCTAACCAAGCCCTTGAGCGCATCTTACAT
TTAGTGCCAAACGATCAAAAAGAGCAGTTTCTATTTGATTTGTCGATGAACTTAAAAGGTGTGATAGGCCAACAACTTAT
CAGAGATAAAAATGGTCAAGGTCGACATGGGGTATTTGAAGTTCTGTTAAATAGCCCGCGAGTTTCTGATTTAATTCGTC
GAGGTGATTTACATGAACTCAAAGCGACGATGGCACGCTCAAATGAATTTGGTATGTTGACGTTCGACCAGTCACTCTAC
AAGTTGGTAATGCAAGGTAAGATCAGCGAGGAGGATGCGTTGCACAGTGCGGATTCTGCAAATGATCTTCGCTTAATGTT
AAAAACTCAGCGCGGAGAATCTTTCTCATCGGGCAGCTTAGAAAACGTTAAGATCGATATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(27-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.88

100

0.829
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

92.12

0.399
  pilT Acinetobacter baumannii strain A118

42.183

92.12

0.389
  pilT Acinetobacter baumannii D1279779

42.183

92.12

0.389
  pilT Acinetobacter nosocomialis M2

42.183

92.12

0.389
  pilT Pseudomonas aeruginosa PAK

41.888

92.12

0.386
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37