Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LDO90_RS16990 Genome accession   NZ_AP025147
Coordinates   3777931..3779037 (-) Length   368 a.a.
NCBI ID   WP_126608180.1    Uniprot ID   A0AAV5NVK2
Organism   Vibrio penaeicida strain IFO 15641     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3772931..3784037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LDO90_RS16960 - 3773676..3774176 (+) 501 WP_126608177.1 SprT family zinc-dependent metalloprotease -
  LDO90_RS16965 - 3774263..3774964 (+) 702 WP_126608178.1 endonuclease -
  LDO90_RS16970 rsmE 3775063..3775794 (+) 732 WP_126608179.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LDO90_RS16975 gshB 3775809..3776759 (+) 951 WP_101112561.1 glutathione synthase -
  LDO90_RS16980 - 3776795..3777358 (+) 564 WP_101112562.1 YqgE/AlgH family protein -
  LDO90_RS16985 ruvX 3777405..3777833 (+) 429 WP_101112582.1 Holliday junction resolvase RuvX -
  LDO90_RS16990 pilU 3777931..3779037 (-) 1107 WP_126608180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LDO90_RS16995 pilT 3779046..3780083 (-) 1038 WP_101112564.1 type IV pilus twitching motility protein PilT Machinery gene
  LDO90_RS17000 - 3780107..3780817 (+) 711 WP_126608181.1 YggS family pyridoxal phosphate-dependent enzyme -
  LDO90_RS17005 proC 3780880..3781698 (+) 819 WP_126608182.1 pyrroline-5-carboxylate reductase -
  LDO90_RS17010 - 3781727..3782284 (+) 558 WP_126608183.1 YggT family protein -
  LDO90_RS17015 yggU 3782284..3782574 (+) 291 WP_126608184.1 DUF167 family protein YggU -
  LDO90_RS17020 - 3782598..3783029 (+) 432 WP_126608185.1 DUF4426 domain-containing protein -
  LDO90_RS17025 - 3783120..3783719 (+) 600 WP_101112570.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41289.25 Da        Isoelectric Point: 6.0176

>NTDB_id=90009 LDO90_RS16990 WP_126608180.1 3777931..3779037(-) (pilU) [Vibrio penaeicida strain IFO 15641]
MELNQVLSEMVAQKASDIYITVGAPVLLRVHGELQPLGEVLTETQAFSLLDSMMDEERRGDYRKNKESNFAIVRDVGRFR
VSAFFQRELPGAVIRRIETQIPTFEDLRLPDILQDLSIAKRGLVLVVGATGSGKSTTMAAMTGFRNQNRTGHILTVEDPI
EFVHEHNKCIITQREVGLDTDSYEIALKNSLRQAPDMILIGEIRSRETMQYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLRGVIAQQLIRDKSGKGRHGVFEILLNTPRVSDLIRRGELHELKATMSKSNESGMKTFDQCLY
ELVVGDKITEQDAMHSADSANDLRLMLKTNRGDDFSGGALDGVKIDMN

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=90009 LDO90_RS16990 WP_126608180.1 3777931..3779037(-) (pilU) [Vibrio penaeicida strain IFO 15641]
ATGGAACTGAATCAAGTCTTATCTGAAATGGTGGCTCAGAAAGCCTCAGATATCTACATTACCGTTGGTGCACCTGTTTT
GCTGCGAGTCCATGGCGAATTGCAGCCATTGGGAGAAGTGTTGACGGAAACACAAGCCTTTTCCCTGCTCGATAGCATGA
TGGATGAAGAGCGACGAGGCGATTACCGTAAAAATAAAGAGTCCAACTTCGCGATTGTTCGCGATGTGGGTCGCTTTAGG
GTCAGTGCTTTTTTCCAGCGTGAATTACCAGGAGCGGTAATACGCCGAATTGAAACTCAAATACCTACCTTTGAGGATCT
TCGATTACCCGATATCTTACAAGACTTGTCTATTGCTAAACGTGGTTTGGTGCTGGTGGTTGGTGCTACAGGTTCAGGTA
AATCAACCACCATGGCAGCCATGACAGGCTTTCGTAATCAAAACCGAACAGGGCACATTCTGACGGTTGAGGATCCTATT
GAATTTGTTCACGAGCACAACAAGTGCATCATCACACAACGTGAAGTTGGGCTGGATACCGATTCTTATGAAATTGCGTT
GAAGAACTCGCTTCGTCAAGCGCCAGATATGATCTTGATTGGTGAAATTCGTTCACGAGAGACCATGCAATACGCCATGA
CATTTGCTGAGACTGGGCACTTGTGTATGGCGACTCTGCACGCCAATAACGCCAACCAAGCACTAGAGCGAATTCTTCAT
TTAGTGCCTAAAGAGCAAAAAGAGCAATTCCTATTCGATCTCTCCATGAACCTCCGAGGAGTGATCGCTCAGCAATTGAT
CCGGGATAAGAGCGGTAAAGGGCGTCATGGGGTGTTTGAGATCTTATTGAATACGCCAAGGGTATCTGATCTTATTCGTC
GCGGAGAATTGCATGAGCTAAAAGCGACGATGTCGAAATCGAATGAATCGGGTATGAAAACCTTCGATCAGTGTTTGTAC
GAGCTGGTGGTTGGCGACAAGATTACAGAGCAAGACGCCATGCACAGCGCCGATTCGGCAAACGATTTAAGACTAATGCT
GAAAACCAACCGTGGAGACGATTTCTCTGGTGGCGCATTGGACGGTGTGAAGATCGATATGAATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

78.747

99.728

0.785
  pilU Pseudomonas stutzeri DSM 10701

56.857

95.109

0.541
  pilU Acinetobacter baylyi ADP1

53.561

95.38

0.511
  pilT Acinetobacter nosocomialis M2

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

41.593

92.12

0.383
  pilT Acinetobacter baumannii strain A118

41.593

92.12

0.383
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.901

88.043

0.378
  pilT Legionella pneumophila strain Lp02

40.708

92.12

0.375
  pilT Legionella pneumophila strain ERS1305867

40.708

92.12

0.375
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Pseudomonas aeruginosa PAK

40.118

92.12

0.37
  pilT Acinetobacter baylyi ADP1

41.846

88.315

0.37
  pilT Pseudomonas stutzeri DSM 10701

39.233

92.12

0.361

Multiple sequence alignment