Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4699_RS10215 Genome accession   NZ_CP137105
Coordinates   2033437..2034378 (+) Length   313 a.a.
NCBI ID   WP_050104907.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H2092     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2028437..2039378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4699_RS10195 - 2028503..2030320 (+) 1818 WP_001220922.1 acyltransferase family protein -
  R4699_RS10200 nagA 2030473..2031624 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4699_RS10205 - 2031787..2032845 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R4699_RS10210 - 2032996..2033361 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4699_RS10215 comGA/cglA/cilD 2033437..2034378 (+) 942 WP_050104907.1 competence type IV pilus ATPase ComGA Machinery gene
  R4699_RS10220 comGB/cglB 2034326..2035342 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4699_RS10225 comGC/cglC 2035344..2035667 (+) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  R4699_RS10230 comGD/cglD 2035630..2036064 (+) 435 WP_000608686.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4699_RS10235 comGE/cglE 2036027..2036329 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4699_RS10240 comGF/cglF 2036292..2036753 (+) 462 WP_000250520.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4699_RS10245 comGG/cglG 2036731..2037144 (+) 414 WP_000265615.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4699_RS10250 - 2037281..2037868 (+) 588 WP_000679776.1 class I SAM-dependent methyltransferase -
  R4699_RS10255 comYH 2037929..2038882 (+) 954 WP_044813830.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35572.49 Da        Isoelectric Point: 5.8526

>NTDB_id=895512 R4699_RS10215 WP_050104907.1 2033437..2034378(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16H2092]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMYELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895512 R4699_RS10215 WP_050104907.1 2033437..2034378(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16H2092]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCTTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGTATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

66.238

99.361

0.658
  comYA Streptococcus mutans UA140

66.238

99.361

0.658
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.903

99.042

0.623
  comGA Lactococcus lactis subsp. cremoris KW2

54.459

100

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.279

86.901

0.367