NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	R4699_RS10240	Genome accession	NZ_CP137105
Coordinates	2036292..2036753 (+)	Length	153 a.a.
NCBI ID	WP_000250520.1	Uniprot ID	C1CA95
Organism	Streptococcus pneumoniae strain 16H2092
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2031292..2041753

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R4699_RS10205	-	2031787..2032845 (+)	1059	WP_000649471.1	zinc-dependent alcohol dehydrogenase family protein	-
R4699_RS10210	-	2032996..2033361 (+)	366	WP_000286415.1	DUF1033 family protein	-
R4699_RS10215	comGA/cglA/cilD	2033437..2034378 (+)	942	WP_050104907.1	competence type IV pilus ATPase ComGA	Machinery gene
R4699_RS10220	comGB/cglB	2034326..2035342 (+)	1017	WP_074017570.1	competence type IV pilus assembly protein ComGB	Machinery gene
R4699_RS10225	comGC/cglC	2035344..2035667 (+)	324	WP_000738628.1	comG operon protein ComGC	Machinery gene
R4699_RS10230	comGD/cglD	2035630..2036064 (+)	435	WP_000608686.1	competence type IV pilus minor pilin ComGD	Machinery gene
R4699_RS10235	comGE/cglE	2036027..2036329 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
R4699_RS10240	comGF/cglF	2036292..2036753 (+)	462	WP_000250520.1	competence type IV pilus minor pilin ComGF	Machinery gene
R4699_RS10245	comGG/cglG	2036731..2037144 (+)	414	WP_000265615.1	competence type IV pilus minor pilin ComGG	Machinery gene
R4699_RS10250	-	2037281..2037868 (+)	588	WP_000679776.1	class I SAM-dependent methyltransferase	-
R4699_RS10255	comYH	2037929..2038882 (+)	954	WP_044813830.1	class I SAM-dependent methyltransferase	Machinery gene
R4699_RS10260	-	2038933..2040123 (+)	1191	WP_050085692.1	acetate kinase	-
R4699_RS10265	-	2040124..2040255 (+)	132	WP_000768904.1	hypothetical protein	-
R4699_RS10270	rnpA	2040272..2040643 (+)	372	WP_000739246.1	ribonuclease P protein component	-
R4699_RS10275	-	2040618..2041442 (+)	825	WP_000727904.1	membrane protein insertase YidC	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 17905.50 Da Isoelectric Point: 8.0031

                                >NTDB_id=895517 R4699_RS10240 WP_000250520.1 2036292..2036753(+) (comGF/cglF) [Streptococcus pneumoniae strain 16H2092]

MVQNSCWQSKSHKVKAFTLLESLIALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN

RLYMKQDGKEIAMGKSKADDFRKTNARGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

Download Length: 462 bp

                                >NTDB_id=895517 R4699_RS10240 WP_000250520.1 2036292..2036753(+) (comGF/cglF) [Streptococcus pneumoniae strain 16H2092]

ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGATTGCCCTGAT

TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT

CGCCTATACATGAAGCAAGATGGCAAGGAGATTGCTATGGGTAAGTCTAAGGCAGATGATTTCCGTAAAACGAATGCTCG

TGGTCGAGGTTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT

TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	C1CA95

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae D39	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae R6	93.464	100	0.935
comGF/cglF	Streptococcus pneumoniae TIGR4	93.464	100	0.935
comGF/cglF	Streptococcus mitis NCTC 12261	90.85	100	0.909
comGF/cglF	Streptococcus mitis SK321	90.85	100	0.909

Detailed information NTG0895517