Detailed information    

experimental Experimentally validated

Overview

Name   recN   Type   Machinery gene
Locus tag   BSU_24240 Genome accession   NC_000964
Coordinates   2520557..2522287 (-) Length   576 a.a.
NCBI ID   NP_390304.2    Uniprot ID   P17894
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

In the presence of ATP, RecN tethers the 3'-ssDNA ends, and facilitates the access of RecA to the high local concentration of DNA ends. Then, the resulting RecA nucleoprotein filaments, on different ssDNA segments, might promote the simultaneous genome-wide homology search.


Genomic Context

Location: 2515557..2527287
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_24200 (BSU24200) yqiH 2515614..2515907 (-) 294 NP_390300.2 putative lipoprotein -
  BSU_24205 (BSU24205) - 2516215..2516367 (+) 153 YP_009513977.1 hypothetical protein -
  BSU_24210 (BSU24210) yqiG 2516440..2517558 (+) 1119 NP_390301.1 putative NADH-dependent flavin oxidoreductase -
  BSU_24220 (BSU24220) spo0A 2518023..2518826 (-) 804 NP_390302.1 response regulator, phosphorylated in response to complex YlbF/YmcA/YaaT Regulator
  BSU_24230 (BSU24230) spoIVB 2519102..2520382 (-) 1281 NP_390303.2 regulatory membrane-associated serine protease -
  BSU_24240 (BSU24240) recN 2520557..2522287 (-) 1731 NP_390304.2 factor for double strand breaks DNA repair and genetic recombination Machinery gene
  BSU_24250 (BSU24250) argR 2522324..2522773 (-) 450 NP_390305.1 transcriptional regulator (AhrC(ArgR)-arginine) -
  BSU_24260 (BSU24260) yqxC 2522871..2523716 (-) 846 NP_390306.2 putative 2'-O-ribose RNA methyltransferase -
  BSU_24270 (BSU24270) dxs 2523713..2525614 (-) 1902 NP_390307.1 1-deoxyxylulose-5-phosphate synthase -
  BSU_24280 (BSU24280) ispA 2525789..2526679 (-) 891 NP_390308.2 farnesyl diphosphate synthase -
  BSU_24290 (BSU24290) xseB 2526669..2526923 (-) 255 NP_390309.2 exodeoxyribonuclease VII (small subunit) -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 64508.35 Da        Isoelectric Point: 4.7697

>NTDB_id=352 BSU_24240 NP_390304.2 2520557..2522287(-) (recN) [Bacillus subtilis subsp. subtilis str. 168]
MLAELSIKNFAIIEELTVSFERGLTVLTGETGAGKSIIIDAISLLVGGRGSSEFVRYGEAKAELEGLFLLESGHPVLGVC
AEQGIDVSDEMIVMRRDISTSGKSVCRVNGKLVTIASLREIGRLLLDIHGQHDNQLLMEDENHLQLLDKFAGAEVESALK
TYQEGYQRYVKLLKKLKQLSESEQEMAHRLDLIQFQLEEIESAKLELNEDEQLQEERQQISNFEKIYESLQNAYNALRSE
QGGLDWVGMASAQLEDISDINEPLKKMSESVSNSYYLLEDATFQMRNMLDELEFDPERLNYIETRLNEIKQLKRKYGATV
EDILEYASKIEEEIDQIENRDSHLQSLKKELDSVGKDVAVEAANVSQIRKTWAKKLADEIHRELKSLYMEKSTFDTEFKV
RTASRNEEAPLVNGQPVQLTEQGIDLVKFLISTNTGEPLKSLSKVASGGELSRVMLAIKSIFSSQQDVTSIIFDEVDTGV
SGRVAQAIAEKIHKVSIGSQVLCITHLPQVAAMADTHLYIAKELKDGRTTTRVKPLSKQEKVAEIGRMIAGVEVTDLTKR
HAKELLKQADQVKTTG

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=352 BSU_24240 NP_390304.2 2520557..2522287(-) (recN) [Bacillus subtilis subsp. subtilis str. 168]
TTGTTAGCTGAACTATCGATTAAAAACTTTGCCATTATTGAGGAACTGACGGTTTCTTTTGAACGCGGGCTTACGGTTTT
AACAGGAGAAACCGGAGCCGGCAAATCCATCATCATAGATGCCATTTCTCTTTTAGTCGGAGGCCGCGGATCATCTGAAT
TTGTTAGATATGGGGAAGCAAAAGCCGAACTTGAAGGGCTGTTTCTGTTGGAGAGCGGCCATCCTGTTTTAGGCGTATGC
GCTGAGCAGGGAATCGACGTTTCAGACGAAATGATCGTCATGAGAAGGGATATCAGCACGAGCGGGAAAAGCGTTTGCCG
TGTCAATGGCAAGCTTGTCACGATTGCTTCACTAAGAGAGATCGGCAGGCTTTTATTAGATATTCACGGGCAGCACGATA
ACCAGCTGTTAATGGAAGACGAAAATCATCTCCAGCTTTTGGACAAGTTTGCCGGGGCCGAAGTGGAAAGCGCGCTCAAA
ACGTATCAGGAAGGGTACCAGCGGTATGTGAAGCTGCTCAAAAAGCTTAAGCAGCTTTCTGAAAGCGAACAGGAAATGGC
CCATCGTCTTGATCTTATTCAATTTCAGCTCGAAGAAATCGAATCGGCAAAGCTTGAGCTTAACGAAGATGAACAGCTCC
AAGAAGAGCGCCAGCAAATTTCAAACTTTGAGAAGATATATGAGTCGCTGCAAAATGCATATAACGCGCTTCGCAGTGAG
CAGGGCGGATTGGACTGGGTTGGAATGGCATCAGCGCAGCTTGAAGATATATCTGATATAAACGAACCGCTGAAAAAAAT
GTCTGAAAGTGTTTCGAACTCCTACTATTTGCTTGAGGATGCCACATTTCAGATGAGAAATATGCTGGATGAGCTTGAAT
TTGATCCGGAACGCCTGAACTATATTGAGACGCGACTTAATGAAATCAAACAGCTGAAACGAAAATACGGTGCCACTGTT
GAAGACATTTTGGAATATGCTTCTAAAATTGAAGAAGAGATTGACCAAATTGAAAACAGGGACAGCCACCTTCAAAGCTT
GAAAAAGGAGCTTGACTCCGTCGGCAAAGATGTCGCAGTGGAAGCGGCAAATGTGTCACAAATCCGAAAAACATGGGCGA
AAAAGCTGGCTGATGAGATTCATCGGGAATTAAAAAGCCTGTACATGGAAAAATCAACTTTTGATACGGAATTTAAGGTC
AGAACAGCGTCACGCAATGAAGAGGCGCCGCTTGTAAACGGCCAGCCCGTTCAGCTGACTGAACAAGGGATTGACCTGGT
GAAGTTTTTAATTTCAACCAATACAGGCGAGCCGCTGAAATCCCTTTCGAAAGTCGCTTCAGGTGGAGAGCTGTCACGGG
TGATGCTTGCCATAAAAAGCATTTTTTCCTCACAACAGGATGTGACATCGATCATTTTTGACGAGGTTGATACCGGTGTA
AGCGGCAGGGTCGCACAGGCAATTGCTGAGAAGATCCATAAGGTGTCAATAGGATCACAAGTGCTGTGCATTACACACCT
GCCTCAGGTGGCTGCCATGGCCGATACGCATCTTTATATTGCGAAGGAGTTAAAAGACGGCAGAACGACGACACGTGTCA
AGCCGCTCTCTAAGCAGGAAAAGGTAGCGGAAATCGGGCGCATGATTGCAGGTGTCGAAGTAACCGACTTAACGAAACGC
CATGCGAAAGAACTTTTAAAACAAGCGGATCAAGTCAAAACAACTGGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_23)

(4-228)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P17894

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Humberto Sanchez et al. (2008) Dynamic structures of Bacillus subtilis RecN-DNA complexes. Nucleic Acids Research 36(1):110-20. [PMID: 17999999]