Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R4699_RS10220 Genome accession   NZ_CP137105
Coordinates   2034326..2035342 (+) Length   338 a.a.
NCBI ID   WP_074017570.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16H2092     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2029326..2040342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4699_RS10200 nagA 2030473..2031624 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4699_RS10205 - 2031787..2032845 (+) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R4699_RS10210 - 2032996..2033361 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4699_RS10215 comGA/cglA/cilD 2033437..2034378 (+) 942 WP_050104907.1 competence type IV pilus ATPase ComGA Machinery gene
  R4699_RS10220 comGB/cglB 2034326..2035342 (+) 1017 WP_074017570.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4699_RS10225 comGC/cglC 2035344..2035667 (+) 324 WP_000738628.1 comG operon protein ComGC Machinery gene
  R4699_RS10230 comGD/cglD 2035630..2036064 (+) 435 WP_000608686.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4699_RS10235 comGE/cglE 2036027..2036329 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4699_RS10240 comGF/cglF 2036292..2036753 (+) 462 WP_000250520.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4699_RS10245 comGG/cglG 2036731..2037144 (+) 414 WP_000265615.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4699_RS10250 - 2037281..2037868 (+) 588 WP_000679776.1 class I SAM-dependent methyltransferase -
  R4699_RS10255 comYH 2037929..2038882 (+) 954 WP_044813830.1 class I SAM-dependent methyltransferase Machinery gene
  R4699_RS10260 - 2038933..2040123 (+) 1191 WP_050085692.1 acetate kinase -
  R4699_RS10265 - 2040124..2040255 (+) 132 WP_000768904.1 hypothetical protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38482.48 Da        Isoelectric Point: 9.6162

>NTDB_id=895513 R4699_RS10220 WP_074017570.1 2034326..2035342(+) (comGB/cglB) [Streptococcus pneumoniae strain 16H2092]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=895513 R4699_RS10220 WP_074017570.1 2034326..2035342(+) (comGB/cglB) [Streptococcus pneumoniae strain 16H2092]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGGTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCATTGGTTTTTATTTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae D39

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae R6

98.521

100

0.985
  comGB/cglB Streptococcus pneumoniae TIGR4

98.521

100

0.985
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.083

100

0.941
  comYB Streptococcus gordonii str. Challis substr. CH1

70.536

99.408

0.701
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503