Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QYQ95_RS02115 Genome accession   NZ_CP135176
Coordinates   423032..424141 (+) Length   369 a.a.
NCBI ID   WP_016800636.1    Uniprot ID   A0AAN0LRV4
Organism   Vibrio cyclitrophicus ZF270     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 418032..429141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ95_RS02080 (QYQ95_02080) - 418254..418853 (-) 600 WP_010435646.1 XTP/dITP diphosphatase -
  QYQ95_RS02085 (QYQ95_02085) - 418902..419333 (-) 432 WP_342202983.1 DUF4426 domain-containing protein -
  QYQ95_RS02090 (QYQ95_02090) yggU 419364..419654 (-) 291 WP_010435651.1 DUF167 family protein YggU -
  QYQ95_RS02095 (QYQ95_02095) - 419684..420241 (-) 558 WP_010435653.1 YggT family protein -
  QYQ95_RS02100 (QYQ95_02100) proC 420318..421136 (-) 819 WP_010435654.1 pyrroline-5-carboxylate reductase -
  QYQ95_RS02105 (QYQ95_02105) - 421249..421953 (-) 705 WP_010435657.1 YggS family pyridoxal phosphate-dependent enzyme -
  QYQ95_RS02110 (QYQ95_02110) pilT 421980..423017 (+) 1038 WP_010435660.1 type IV pilus twitching motility protein PilT Machinery gene
  QYQ95_RS02115 (QYQ95_02115) pilU 423032..424141 (+) 1110 WP_016800636.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QYQ95_RS02120 (QYQ95_02120) - 424198..424359 (-) 162 WP_016785941.1 hypothetical protein -
  QYQ95_RS02125 (QYQ95_02125) ruvX 424458..424880 (-) 423 WP_004735480.1 Holliday junction resolvase RuvX -
  QYQ95_RS02130 (QYQ95_02130) - 424929..425492 (-) 564 WP_016768176.1 YqgE/AlgH family protein -
  QYQ95_RS02135 (QYQ95_02135) gshB 425609..426559 (-) 951 WP_004735482.1 glutathione synthase -
  QYQ95_RS02140 (QYQ95_02140) rsmE 426569..427300 (-) 732 WP_010435671.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QYQ95_RS02145 (QYQ95_02145) - 427445..428176 (-) 732 WP_010435675.1 endonuclease -
  QYQ95_RS02150 (QYQ95_02150) - 428273..428773 (-) 501 WP_016800637.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41322.33 Da        Isoelectric Point: 6.0672

>NTDB_id=885964 QYQ95_RS02115 WP_016800636.1 423032..424141(+) (pilU) [Vibrio cyclitrophicus ZF270]
MELNQILEGMLSQKASDLYITVDAPVLFRIDGELRPQGEKLNAAQVALLLDAMMEQDRRDEYKQTREANFAIVRDFGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPDVLQDLSIAKRGLVLVVGATGSGKSTSMAAMTGYRNTNRSGHILTVEDPI
EFVHEHKKCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRTRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQKDQFLFDLSMNLRGVVAQQLIRDKNGSGRHGVFEILLNSPRVSDLIRRGELHELKSTMAKSKEIGMQTFDQALY
DLVVAGKISEEDAFHSADSANDLRLMLKTKRGDDDYGVGALAGVKIDMG

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=885964 QYQ95_RS02115 WP_016800636.1 423032..424141(+) (pilU) [Vibrio cyclitrophicus ZF270]
ATGGAATTGAATCAAATTCTTGAAGGTATGCTTTCACAAAAAGCGTCGGATCTTTACATCACGGTAGATGCACCGGTTCT
GTTTCGTATTGATGGTGAATTGCGACCTCAGGGAGAGAAACTGAATGCGGCTCAGGTTGCGCTGTTACTGGATGCGATGA
TGGAGCAAGACAGGCGCGACGAGTACAAACAAACGCGTGAGGCTAACTTTGCTATTGTGCGTGATTTTGGTCGTTTTCGT
GTTAGCGCATTCTTTCAGCGAGAGCTACCAGGAGCGGTAATTCGACGTATCGAGACTAACATCCCAACCTTCGAACAATT
AAAGCTTCCAGACGTACTACAAGACCTTTCAATCGCAAAACGCGGACTTGTGTTGGTGGTTGGGGCTACAGGTTCTGGTA
AATCAACCTCAATGGCTGCGATGACGGGGTATCGCAATACCAACCGTTCGGGGCATATCTTGACGGTTGAAGACCCGATT
GAGTTCGTGCACGAACATAAAAAGTGCATTGTGACTCAGCGAGAAGTTGGGCTCGATACCGAGAGCTATGAAGTCGCGCT
TAAGAACTCGTTACGCCAAGCGCCTGACATGATCTTGATTGGTGAAATCCGTACCCGTGAAACCATGGAGTACGCGATGA
CGTTTGCTGAAACCGGTCACCTTTGCATGGCGACTTTACATGCCAACAATGCCAACCAAGCGCTAGAGCGTATTCTTCAC
TTGGTGCCGAAAGAGCAAAAAGATCAGTTCTTATTTGATTTGTCGATGAATCTACGTGGTGTCGTTGCTCAGCAATTAAT
CCGAGATAAGAATGGTAGTGGTCGCCATGGTGTGTTCGAAATCCTACTCAACAGCCCACGGGTGTCTGATTTGATTCGTA
GAGGTGAGTTACATGAACTCAAGTCGACAATGGCTAAATCAAAAGAGATTGGGATGCAGACCTTTGACCAAGCTTTGTAT
GACTTAGTCGTTGCAGGCAAGATCAGTGAAGAGGATGCGTTCCATAGTGCCGATTCCGCTAATGATTTGCGCTTGATGCT
CAAGACTAAACGTGGTGATGACGATTACGGAGTGGGAGCCTTAGCGGGAGTTAAAATTGATATGGGTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

83.696

99.729

0.835
  pilU Pseudomonas stutzeri DSM 10701

58.571

94.851

0.556
  pilU Acinetobacter baylyi ADP1

54.131

95.122

0.515
  pilT Acinetobacter baumannii D1279779

42.105

92.683

0.39
  pilT Acinetobacter baumannii strain A118

42.105

92.683

0.39
  pilT Acinetobacter nosocomialis M2

42.105

92.683

0.39
  pilT Legionella pneumophila strain ERS1305867

42.183

91.87

0.388
  pilT Legionella pneumophila strain Lp02

42.183

91.87

0.388
  pilT Pseudomonas aeruginosa PAK

41.593

91.87

0.382
  pilT Acinetobacter baylyi ADP1

42.169

89.973

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.805

0.379
  pilT Vibrio cholerae strain A1552

41.317

90.515

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.317

90.515

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.071

91.057

0.374