Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RKE25_RS19445 Genome accession   NZ_CP134867
Coordinates   4427489..4428604 (+) Length   371 a.a.
NCBI ID   WP_311839733.1    Uniprot ID   A0AA96EKR6
Organism   Dyella sp. BiH032     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4422489..4433604
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE25_RS19425 (RKE25_19425) - 4424076..4425446 (-) 1371 WP_311839729.1 pyridoxal-phosphate dependent enzyme -
  RKE25_RS19430 (RKE25_19430) - 4425618..4425848 (-) 231 WP_311839730.1 YdcH family protein -
  RKE25_RS19435 (RKE25_19435) - 4426188..4426820 (-) 633 WP_311839731.1 hypothetical protein -
  RKE25_RS19440 (RKE25_19440) - 4426817..4427401 (-) 585 WP_311839732.1 DUF4398 domain-containing protein -
  RKE25_RS19445 (RKE25_19445) pilU 4427489..4428604 (+) 1116 WP_311839733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RKE25_RS19450 (RKE25_19450) maiA 4428852..4429517 (-) 666 WP_311839734.1 maleylacetoacetate isomerase -
  RKE25_RS19455 (RKE25_19455) - 4429657..4430835 (+) 1179 WP_311839735.1 phosphoglycerate kinase -
  RKE25_RS19460 (RKE25_19460) - 4430847..4431479 (+) 633 WP_311842442.1 HAD hydrolase-like protein -
  RKE25_RS19465 (RKE25_19465) - 4431532..4432407 (+) 876 WP_311839736.1 LysR family transcriptional regulator -
  RKE25_RS19470 (RKE25_19470) - 4432450..4433469 (-) 1020 WP_311839737.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 41151.39 Da        Isoelectric Point: 6.3525

>NTDB_id=884218 RKE25_RS19445 WP_311839733.1 4427489..4428604(+) (pilU) [Dyella sp. BiH032]
MDIGYFLKLMVDKGASDMFLTTGAPVNIKVEGKLYPLGNTGLPGGMVKKIAYSLMDEGQVPQFERDLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSDIPSIDQLQLPGIFRELIMEPRGLILVVGATGSGKSTTLAAMLDHRNQNSSGHILTIED
PIEYLHRHKKSIINQREVGLDTHSYHEALKNAMREAPDVIMIGEIRDTDTMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRLPATEVLLNTPFIRDMIRRGQIHEVKEAMDRSLQEGMQTFDQSL
YRLFKEGRIELEEALAKADSRDGLALKIRLSEGGGSDSAELVGNDPYGLGF

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=884218 RKE25_RS19445 WP_311839733.1 4427489..4428604(+) (pilU) [Dyella sp. BiH032]
GTGGATATTGGTTACTTCCTCAAGCTGATGGTGGACAAGGGCGCGTCGGACATGTTCCTGACGACCGGCGCCCCCGTGAA
CATCAAGGTGGAGGGCAAGCTGTACCCGCTGGGCAACACCGGCCTGCCCGGCGGCATGGTGAAGAAGATCGCCTATTCGC
TCATGGACGAGGGCCAGGTGCCTCAGTTCGAACGCGACCTGGAGCTCAACATGGCGCTGGCGGTCAAGGAGGCCGGCCGC
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGCGCGATCAAGAGCGACATCCCCAGCATCGA
CCAGTTGCAGCTGCCGGGCATTTTCCGCGAGCTGATCATGGAGCCGCGCGGCCTCATCCTGGTGGTCGGCGCCACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGCTCGACCATCGCAACCAGAACTCGTCGGGCCACATCCTCACCATCGAGGAT
CCCATCGAGTATCTGCATCGCCACAAGAAGTCGATCATCAACCAGCGCGAAGTCGGCCTCGACACGCACAGCTATCACGA
GGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCATGATCGGCGAGATCCGCGACACGGACACCATGGAAGCGG
CCATCGCCTTCTCCGAGACGGGCCATCTCTGTCTCGCCACGCTGCACTCCAACAATGCGGACCAGACGCTGGAGCGCATC
CTCAACTTCTTCCCGGAATCGGCGCACAAGAACGTGCTGATGAATCTCGCGCTGAATCTGCGCGCGGTGATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGCCGCCGCCTGCCGGCCACCGAGGTGCTGCTCAACACGCCGTTCATCCGCGACATGATCC
GCCGCGGCCAGATCCACGAGGTGAAGGAAGCCATGGATCGCAGCCTGCAGGAAGGCATGCAGACCTTCGACCAGTCGCTG
TACCGGCTTTTCAAGGAAGGCCGCATTGAGCTGGAGGAGGCGCTGGCCAAGGCCGATTCGCGCGATGGCCTGGCGCTCAA
GATTCGCCTTTCCGAAGGCGGCGGCTCGGACAGCGCGGAGCTGGTCGGCAACGACCCCTACGGCCTGGGTTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.261

96.226

0.493
  pilU Acinetobacter baylyi ADP1

51.13

95.418

0.488
  pilU Vibrio cholerae strain A1552

48.895

97.574

0.477
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.838

91.375

0.41
  pilT Acinetobacter baylyi ADP1

42.353

91.644

0.388
  pilT Acinetobacter nosocomialis M2

41.716

91.105

0.38
  pilT Acinetobacter baumannii strain A118

41.716

91.105

0.38
  pilT Acinetobacter baumannii D1279779

41.716

91.105

0.38
  pilT Pseudomonas aeruginosa PAK

41.124

91.105

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.828

91.105

0.372
  pilT Vibrio cholerae strain A1552

40.059

90.836

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.059

90.836

0.364
  pilT Legionella pneumophila strain Lp02

40.729

88.679

0.361
  pilT Legionella pneumophila strain ERS1305867

40.729

88.679

0.361