Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   MPHASIOC01_RS16800 Genome accession   NZ_CP134782
Coordinates   3488775..3489752 (-) Length   325 a.a.
NCBI ID   WP_064601228.1    Uniprot ID   A0A1B7KZ01
Organism   Mangrovibacter phragmitis strain ASIOC01     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3483775..3494752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPHASIOC01_RS16770 (MPHASIOC01_003337) endA 3484174..3484884 (+) 711 WP_064601222.1 deoxyribonuclease I -
  MPHASIOC01_RS16775 (MPHASIOC01_003338) rsmE 3485030..3485761 (+) 732 WP_064601223.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  MPHASIOC01_RS16780 (MPHASIOC01_003339) gshB 3485773..3486720 (+) 948 WP_353612955.1 glutathione synthase -
  MPHASIOC01_RS16785 (MPHASIOC01_003340) - 3486832..3487395 (+) 564 WP_064601225.1 YqgE/AlgH family protein -
  MPHASIOC01_RS16790 (MPHASIOC01_003341) ruvX 3487395..3487814 (+) 420 WP_353612956.1 Holliday junction resolvase RuvX -
  MPHASIOC01_RS16795 (MPHASIOC01_003342) - 3488213..3488425 (+) 213 WP_064601227.1 DUF1471 domain-containing protein -
  MPHASIOC01_RS16800 (MPHASIOC01_003343) pilT 3488775..3489752 (-) 978 WP_064601228.1 type IV pilus twitching motility protein PilT Machinery gene
  MPHASIOC01_RS16805 (MPHASIOC01_003344) - 3489773..3490486 (+) 714 WP_064601229.1 YggS family pyridoxal phosphate-dependent enzyme -
  MPHASIOC01_RS16810 (MPHASIOC01_003345) - 3490502..3491056 (+) 555 WP_036102866.1 YggT family protein -
  MPHASIOC01_RS16815 (MPHASIOC01_003346) yggU 3491053..3491343 (+) 291 WP_064601230.1 DUF167 family protein YggU -
  MPHASIOC01_RS16820 (MPHASIOC01_003347) - 3491359..3491952 (+) 594 WP_064601231.1 XTP/dITP diphosphatase -
  MPHASIOC01_RS16825 (MPHASIOC01_003348) hemW 3491945..3493084 (+) 1140 WP_353612957.1 radical SAM family heme chaperone HemW -
  MPHASIOC01_RS16830 (MPHASIOC01_003349) - 3493286..3494008 (-) 723 WP_064601233.1 DUF2884 family protein -
  MPHASIOC01_RS16835 (MPHASIOC01_003350) - 3494068..3494394 (-) 327 WP_064601234.1 YggL family protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 34859.20 Da        Isoelectric Point: 6.6311

>NTDB_id=883697 MPHASIOC01_RS16800 WP_064601228.1 3488775..3489752(-) (pilT) [Mangrovibacter phragmitis strain ASIOC01]
MEEIVALSVKHNVSDLHLCSSGHFYWRKDGVMEPAPCALPDISALLDLWLTPEQHSQFKHHGQLDFAVELANGQRLRANA
FLQVAGVSLALRILPQVCPELTTLQVPEALMALLQEQSGLVLVCGATGSGKSTTLAAMVGELNRQTQAHIITLEDPVEFV
HKPPQGVIQQREVGLHCASFAAGLKGALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDVFP
AEEKSMVRTQLAGSLKGVVAQQLAARKGGGRVALFELLVVTPAVSSLVREGKTHQLPGVLQTGRQAGMQTFEQSRAERDA
AGMLA

Nucleotide


Download         Length: 978 bp        

>NTDB_id=883697 MPHASIOC01_RS16800 WP_064601228.1 3488775..3489752(-) (pilT) [Mangrovibacter phragmitis strain ASIOC01]
ATGGAAGAAATCGTGGCCCTTAGTGTAAAGCATAATGTGTCGGATCTACACCTGTGTAGCAGTGGGCATTTTTACTGGCG
TAAAGATGGCGTGATGGAGCCAGCCCCGTGTGCTTTACCCGATATATCGGCATTGCTGGATTTGTGGCTGACACCAGAGC
AGCACAGCCAGTTTAAACACCATGGGCAACTTGATTTCGCCGTCGAGCTGGCAAATGGCCAGCGTTTAAGAGCCAATGCT
TTTCTTCAGGTGGCAGGGGTGTCTCTGGCGTTGCGTATTTTACCGCAGGTATGTCCTGAGTTAACCACATTACAGGTTCC
TGAAGCATTAATGGCACTGCTACAAGAGCAGTCAGGGTTGGTATTAGTGTGTGGTGCTACGGGCAGTGGCAAGTCAACGA
CACTGGCAGCCATGGTTGGTGAGCTGAACCGGCAAACGCAGGCACATATTATTACCCTTGAAGACCCGGTTGAGTTTGTT
CACAAACCTCCTCAGGGTGTTATCCAGCAGCGTGAAGTAGGTTTGCATTGTGCCTCTTTTGCTGCCGGGCTGAAGGGGGC
TTTACGCCAGGATCCGGATGTCATTTTGCTGGGCGAGTTGCGAGACAGCGAAACGATTCGCCTCGCCCTGACAGCGGCTG
AAACAGGGCACCTGGTGTTGGCAACACTGCATACCCGTGGGGCTGCACAAGCGGTAGAGCGGCTGGTAGATGTCTTCCCG
GCGGAAGAGAAAAGTATGGTTCGTACTCAACTTGCTGGCAGCCTTAAAGGTGTGGTAGCTCAACAACTGGCTGCGCGTAA
AGGTGGTGGGCGGGTGGCTTTGTTTGAGCTTTTAGTTGTTACGCCAGCCGTTTCCAGCCTGGTTCGGGAAGGGAAAACAC
ACCAGTTACCCGGTGTATTACAGACTGGCAGGCAGGCTGGCATGCAAACTTTTGAACAAAGCCGCGCCGAGCGTGATGCG
GCGGGAATGCTTGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B7KZ01

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.012

100

0.483
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.012

100

0.483
  pilT Neisseria meningitidis 8013

47.17

97.846

0.462
  pilT Neisseria gonorrhoeae MS11

47.17

97.846

0.462
  pilT Acinetobacter baylyi ADP1

44.377

100

0.449
  pilT Pseudomonas stutzeri DSM 10701

46.006

96.308

0.443
  pilT Legionella pneumophila strain ERS1305867

44.969

97.846

0.44
  pilT Legionella pneumophila strain Lp02

44.969

97.846

0.44
  pilT Acinetobacter nosocomialis M2

44.514

98.154

0.437
  pilT Acinetobacter baumannii D1279779

44.514

98.154

0.437
  pilT Acinetobacter baumannii strain A118

44.514

98.154

0.437
  pilT Pseudomonas aeruginosa PAK

45.367

96.308

0.437
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.772

97.231

0.406
  pilU Vibrio cholerae strain A1552

42.02

94.462

0.397