Detailed information NTG0879993

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: RKE53_RS17150
• Genome accession: NZ_CP134413
• Coordinates: 3704230..3705336 (-)
• Length: 368 a.a.
• NCBI ID: WP_104396083.1
• Uniprot ID: -
• Organism: Microcystis aeruginosa NRERC-214
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3699230..3710336

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RKE53_RS17140 (RKE53_17140)	-	3699906..3702476 (+)	2571	WP_311130905.1	TIGR03960 family B12-binding radical SAM protein	-
RKE53_RS17145 (RKE53_17145)	-	3702875..3704089 (-)	1215	WP_104396084.1	type II secretion system F family protein	-
RKE53_RS17150 (RKE53_17150)	pilT	3704230..3705336 (-)	1107	WP_104396083.1	type IV pilus twitching motility protein PilT	Machinery gene
RKE53_RS17155 (RKE53_17155)	pilB/pilB1	3705548..3707560 (-)	2013	WP_311130906.1	GspE/PulE family protein	Machinery gene
RKE53_RS17160 (RKE53_17160)	grpE	3707801..3708523 (+)	723	WP_288137408.1	nucleotide exchange factor GrpE	-
RKE53_RS17165 (RKE53_17165)	-	3708640..3709281 (-)	642	WP_104396080.1	HAD-IA family hydrolase	-

Sequence

Protein

• Length: 368 a.a.
• Molecular weight: 40675.89 Da
• Isoelectric Point: 6.6526

>NTDB_id=879993 RKE53_RS17150 WP_104396083.1 3704230..3705336(-) (pilT) [Microcystis aeruginosa NRERC-214]
MELMIEDLMEQLVEMGGSDMHIQSRAPVYFRISGKLIPVNEESLTPQDCQKLIFSMLNNTQIKELEQNWELDCSYGVKGL
ARFRINVYKERGAYAACLRALSSKIPNFEQLNLPEVIRELSERPRGLILVTGQTGSGKTTTLASILDLINRTRSEHILTV
EDPIEYVFPNIKSLFHQRQKGEDTKSFANALRAALREDPDIILVGEMRDLETISLAITAAETGHLVFGTLHTSSAAGTVD
RIIDVFPSNQQSQIRAMLSNSLLAVFSQNLVKKKNPKPGEFGRAMVQEIMVVTPAIANLIREGKAPQVYSAIQTGMKLGM
QTMEQGLANLVVSGVISFEEAISKSSKPDELQRLVAGSGANAKTKAWI

Nucleotide

• Length: 1107 bp

>NTDB_id=879993 RKE53_RS17150 WP_104396083.1 3704230..3705336(-) (pilT) [Microcystis aeruginosa NRERC-214]
ATGGAATTAATGATCGAAGATTTAATGGAACAATTGGTCGAGATGGGCGGTTCCGATATGCACATTCAGTCCCGTGCGCC
GGTCTATTTTCGCATTAGTGGCAAACTCATCCCGGTTAATGAAGAATCTTTAACACCGCAAGATTGTCAGAAATTGATTT
TTAGTATGCTCAATAATACTCAAATAAAAGAGTTAGAGCAGAATTGGGAATTAGACTGTTCATACGGGGTGAAAGGATTA
GCAAGATTTAGGATCAATGTGTATAAAGAAAGGGGAGCTTATGCAGCTTGTTTACGCGCTTTGTCATCTAAAATTCCTAA
CTTTGAACAATTAAATTTACCAGAAGTGATCCGAGAATTATCGGAAAGACCGAGGGGATTAATTCTCGTAACTGGACAAA
CAGGTTCAGGTAAAACTACCACTTTAGCATCAATTTTAGACTTAATTAATCGGACTAGGTCTGAACACATTTTGACTGTA
GAAGACCCAATCGAATACGTTTTTCCTAATATAAAAAGCCTGTTTCACCAACGACAAAAAGGAGAAGATACCAAGAGTTT
TGCTAACGCATTAAGAGCTGCTTTACGAGAAGATCCTGATATTATCCTTGTGGGAGAAATGCGGGATTTAGAAACAATTA
GTTTGGCAATTACGGCGGCGGAAACCGGTCACTTAGTCTTCGGAACCCTACACACTAGCTCGGCAGCGGGAACAGTGGAT
CGGATCATTGACGTTTTCCCCTCTAACCAACAGTCACAAATTCGCGCCATGTTATCCAATTCTTTACTAGCAGTTTTTAG
TCAAAACCTAGTCAAGAAAAAAAATCCTAAACCGGGGGAATTTGGCCGGGCAATGGTACAGGAAATCATGGTGGTAACTC
CAGCGATCGCTAACCTAATTCGCGAAGGAAAAGCCCCCCAAGTTTACTCAGCAATTCAAACGGGGATGAAGTTAGGAATG
CAAACTATGGAACAGGGATTAGCTAATTTAGTTGTTAGCGGTGTTATATCCTTTGAAGAGGCAATTTCTAAAAGTAGTAA
ACCCGATGAATTACAACGTCTTGTCGCCGGATCTGGTGCTAACGCCAAAACTAAAGCCTGGATTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Legionella pneumophila strain Lp02	51.304	93.75	0.481
pilT	Legionella pneumophila strain ERS1305867	51.304	93.75	0.481
pilT	Pseudomonas aeruginosa PAK	50.144	94.293	0.473
pilT	Pseudomonas stutzeri DSM 10701	49.856	94.293	0.47
pilT	Acinetobacter nosocomialis M2	49.568	94.293	0.467
pilT	Acinetobacter baumannii D1279779	49.568	94.293	0.467
pilT	Acinetobacter baumannii strain A118	49.568	94.293	0.467
pilT	Acinetobacter baylyi ADP1	48.991	94.293	0.462
pilT	Vibrio cholerae strain A1552	49.706	92.391	0.459
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.706	92.391	0.459
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.809	99.457	0.446
pilT	Neisseria meningitidis 8013	48.083	92.12	0.443
pilT	Neisseria gonorrhoeae MS11	47.788	92.12	0.44