Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RKE53_RS17155 Genome accession   NZ_CP134413
Coordinates   3705548..3707560 (-) Length   670 a.a.
NCBI ID   WP_311130906.1    Uniprot ID   -
Organism   Microcystis aeruginosa NRERC-214     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3700548..3712560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RKE53_RS17145 (RKE53_17145) - 3702875..3704089 (-) 1215 WP_104396084.1 type II secretion system F family protein -
  RKE53_RS17150 (RKE53_17150) pilT 3704230..3705336 (-) 1107 WP_104396083.1 type IV pilus twitching motility protein PilT Machinery gene
  RKE53_RS17155 (RKE53_17155) pilB/pilB1 3705548..3707560 (-) 2013 WP_311130906.1 GspE/PulE family protein Machinery gene
  RKE53_RS17160 (RKE53_17160) grpE 3707801..3708523 (+) 723 WP_288137408.1 nucleotide exchange factor GrpE -
  RKE53_RS17165 (RKE53_17165) - 3708640..3709281 (-) 642 WP_104396080.1 HAD-IA family hydrolase -
  RKE53_RS17170 (RKE53_17170) - 3709401..3711365 (-) 1965 WP_311130907.1 amylo-alpha-1,6-glucosidase -
  RKE53_RS17175 (RKE53_17175) rpmH 3711456..3711593 (+) 138 WP_002739276.1 50S ribosomal protein L34 -
  RKE53_RS17180 (RKE53_17180) rnpA 3711620..3711982 (+) 363 WP_104396078.1 ribonuclease P protein component -
  RKE53_RS17185 (RKE53_17185) - 3711972..3712367 (+) 396 WP_104396077.1 PH domain-containing protein -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75190.26 Da        Isoelectric Point: 5.4572

>NTDB_id=879994 RKE53_RS17155 WP_311130906.1 3705548..3707560(-) (pilB/pilB1) [Microcystis aeruginosa NRERC-214]
MTFSSKKTRAVALRNYFSPFGNKLVQSGYIGAEQMQQALVETRKSGRSLVEILQKLTGRPLPPDLQRQYKKNQLFELKIL
YGVESLDPELSDVDGLEIARLIDSLIPIDLCRRYRLIPLRRIEGEPASILIAMVDPDNLEATDDINRILRPRELGLQRLV
ITSEDYERLLEKFHWAQPELEKEKARLEKEKELEKLSDLTDIVASIEVVSGADDEVADDLGEGDANQAPVINLVNKILAK
ALQEGTSDIHIEPQDEFLKIRFRKDGVLHQAFDPLPKRITPAVTARFKIMAELDIAERRLPQDGKIRRIYQGRKVDFRVN
TLPSRYGEKVCLRILDNSSTQLGLDKLITDQKTLQIVRDLASYPFGLLLVTGPTGSGKSTTLYSVLAERNDPGVNISTVE
DPIEYSLPGITQVQVIREKGMDFASILRAFMRQDPDVILVGETRDKETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDE
MGVETFMISGSLLGILAQRLVRRVCTECRVAYHPTQEELARFGLSAANEQEVTFYKANTLTIAEIQQARRQGNLCVKCNG
SGYKGRIGVYEVMQNSERLQQLINQGATTDRIKEAAVDEGMMTLLAYSLNLVREGYTTLEEVERVTFSDSGLEAELKAKR
KSGLICSGCRAELQPEWLDCPYCMKPRFSY

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=879994 RKE53_RS17155 WP_311130906.1 3705548..3707560(-) (pilB/pilB1) [Microcystis aeruginosa NRERC-214]
ATGACATTTTCTTCCAAGAAAACCCGTGCTGTTGCCCTACGCAATTACTTTTCCCCCTTTGGTAACAAGTTGGTGCAGTC
GGGCTATATCGGTGCCGAGCAAATGCAACAGGCCCTGGTGGAAACACGGAAATCGGGACGATCGCTAGTAGAAATATTAC
AAAAACTAACCGGGCGGCCTTTGCCCCCGGATTTACAGCGACAGTATAAGAAAAACCAGTTATTTGAACTAAAAATTCTC
TACGGAGTCGAATCCCTCGACCCCGAATTGAGCGATGTGGATGGACTAGAAATAGCCAGGCTGATCGATTCTTTGATTCC
GATCGATCTTTGTCGTCGCTATCGTCTCATCCCTCTGCGTCGAATCGAAGGAGAGCCGGCTTCAATTTTGATAGCCATGG
TCGATCCCGACAACCTAGAGGCTACGGACGATATTAATCGTATTCTCCGCCCCAGGGAGTTAGGTTTACAGCGTTTAGTG
ATTACCAGCGAAGATTACGAAAGATTACTGGAAAAATTCCACTGGGCCCAACCGGAACTAGAAAAGGAAAAAGCCAGACT
CGAAAAAGAAAAAGAACTGGAAAAACTCTCGGATCTCACCGATATTGTCGCCAGTATTGAGGTGGTCTCTGGTGCTGACG
ATGAAGTGGCCGATGATCTCGGAGAAGGAGACGCTAATCAAGCCCCCGTCATCAACCTGGTCAATAAAATTCTAGCTAAA
GCCCTACAGGAAGGGACTTCCGATATTCACATCGAACCCCAGGACGAATTCCTGAAAATTCGCTTTCGCAAAGATGGAGT
TCTTCATCAAGCTTTTGACCCACTGCCGAAAAGAATTACCCCGGCGGTCACTGCCCGTTTTAAAATCATGGCCGAACTCG
ATATCGCCGAACGTCGTCTTCCCCAAGATGGTAAAATTCGGCGGATTTATCAAGGGCGGAAAGTGGATTTTCGGGTTAAT
ACCCTGCCCAGTCGCTACGGGGAAAAAGTCTGTTTACGGATTCTCGATAACTCCTCTACCCAGTTGGGTTTAGATAAACT
AATCACCGATCAAAAGACCCTGCAAATCGTCCGAGACTTGGCCAGTTATCCTTTTGGTCTGTTGCTGGTGACAGGCCCCA
CTGGTTCGGGGAAATCCACCACTTTATACTCGGTGTTAGCGGAAAGAAATGACCCTGGGGTCAATATCAGTACCGTCGAA
GATCCGATCGAATATTCTCTACCGGGCATCACCCAGGTGCAGGTAATTCGCGAAAAAGGCATGGATTTCGCCTCGATTCT
GCGGGCCTTCATGCGACAAGACCCGGATGTGATTCTGGTGGGAGAAACTAGGGACAAGGAAACGGCTAAAACAGCGATCG
AGGCCGCCTTAACTGGTCACTTGGTCTTAACTACCCTACACACCAACGATGCCGCAGGTGCGATCGCTCGTCTCGATGAG
ATGGGGGTTGAAACGTTTATGATTTCTGGTTCTCTGTTGGGAATTTTGGCCCAGCGTCTGGTGCGGCGCGTCTGTACCGA
GTGTCGAGTCGCCTATCACCCCACCCAAGAGGAATTGGCCCGTTTTGGTCTTTCCGCTGCCAATGAACAGGAAGTGACCT
TTTATAAGGCTAATACCCTGACCATAGCGGAAATTCAACAGGCGCGAAGACAGGGTAATCTCTGCGTTAAGTGTAACGGC
AGCGGTTATAAAGGTCGCATCGGTGTGTATGAGGTGATGCAGAATAGTGAACGACTACAGCAATTAATTAACCAAGGGGC
AACCACCGATCGCATTAAGGAGGCGGCCGTCGATGAGGGTATGATGACACTCCTAGCCTACAGTTTAAATCTGGTGCGCG
AGGGTTACACCACCCTCGAAGAGGTGGAACGGGTGACATTTAGTGATTCTGGTCTGGAAGCGGAATTAAAAGCTAAACGG
AAAAGCGGTTTAATCTGTAGTGGTTGTCGCGCCGAACTACAACCAGAATGGCTCGATTGTCCCTATTGTATGAAACCACG
ATTTAGTTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

73.414

98.806

0.725