Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   RCH27_RS03220 Genome accession   NZ_CP133289
Coordinates   669519..670655 (-) Length   378 a.a.
NCBI ID   WP_011793810.1    Uniprot ID   A1TJV1
Organism   Paracidovorax citrulli strain YDAC2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 664519..675655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RCH27_RS03195 (RCH27_03200) - 665464..665871 (+) 408 WP_011793805.1 YraN family protein -
  RCH27_RS03200 (RCH27_03205) - 665943..666545 (+) 603 WP_011793806.1 SIS domain-containing protein -
  RCH27_RS03205 (RCH27_03210) - 666542..667198 (+) 657 WP_011793807.1 BON domain-containing protein -
  RCH27_RS03210 (RCH27_03215) - 667305..668243 (-) 939 WP_011793808.1 NAD(P)-dependent oxidoreductase -
  RCH27_RS03215 (RCH27_03220) - 668475..669425 (+) 951 WP_011793809.1 IS110 family transposase -
  RCH27_RS03220 (RCH27_03225) pilU 669519..670655 (-) 1137 WP_011793810.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  RCH27_RS03225 (RCH27_03230) - 670684..671322 (-) 639 WP_011793811.1 Crp/Fnr family transcriptional regulator -
  RCH27_RS03230 (RCH27_03235) pilT 671411..672454 (-) 1044 WP_011793812.1 type IV pilus twitching motility protein PilT Machinery gene
  RCH27_RS03235 (RCH27_03240) - 672496..673221 (+) 726 WP_011793813.1 YggS family pyridoxal phosphate-dependent enzyme -
  RCH27_RS03240 (RCH27_03245) ltaE 673254..674318 (-) 1065 WP_011793814.1 low-specificity L-threonine aldolase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41748.16 Da        Isoelectric Point: 6.9079

>NTDB_id=873317 RCH27_RS03220 WP_011793810.1 669519..670655(-) (pilU) [Paracidovorax citrulli strain YDAC2]
MERDQASKFINDLLKLMVGRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARAIMSDKQVADFERTKECNFAI
SPAGIGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEITMSKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMDHAIAFSETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEANVISYEDALRNADSLNDLRLQIKLGSQRARSTDLSSGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=873317 RCH27_RS03220 WP_011793810.1 669519..670655(-) (pilU) [Paracidovorax citrulli strain YDAC2]
ATGGAACGCGATCAGGCCAGCAAATTCATCAACGACCTGCTGAAGCTCATGGTGGGGCGCGGCGGAAGCGATCTTTTCAT
CACGGCGGAGTTCCCTCCCGCCATCAAGGTGGACGGAAAGGTCACGAAGGTATCCCCCCAGCCGCTCACGCCGGCGCACA
CCCTCACGCTCGCGCGCGCCATCATGAGCGACAAGCAGGTGGCGGACTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCCCCCGCGGGCATCGGCCGCTTCCGGGTGAACGCCTTCATCCAGCAGGGCCGCGTCGGCATGGTGCTGCGGACCATCCC
GCTCACGCTGCCCACCATCGACGGGCTGGGCGTGCCGCAGGTCCTCAAGGAAATCACCATGTCCAAGCGCGGGCTGTGCA
TCCTCGTGGGTGCCACCGGCTCGGGCAAGTCCACCACCCTCGCGGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATCACCGTGGAAGACCCGATCGAGTTCGTGCACCCGCACAAGAACTGCGTCGTCACCCAGCGCGAGGTGGGCCT
GGACACCGACAGCTGGGAGGCCGCCCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTCATGGGCGAGATCCGCG
ACCGCGAAACCATGGACCATGCCATCGCGTTCTCCGAGACCGGCCACCTGTGCCTCGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAACGGCGCGCGCAACTGCTCATGGACCTGTCGCTCAACCT
GCGCGCCATGGTGTCGCAGCGCCTCATCCCCAAGCAGGACGGCAAGGGCCGCGCCGCGGCGGTGGAAGTGATGCTCAACA
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTCGGC
ATGCAGACCTTCGACCAGTCGCTGTTCGACCTCTTCGAGGCCAACGTCATCAGCTACGAAGACGCGCTGCGCAACGCCGA
CTCGCTCAACGACCTGCGGCTGCAGATCAAGCTCGGCAGCCAGCGCGCCCGCAGCACCGACCTCTCATCGGGTACCGAGC
ACTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1TJV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

92.328

0.595
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter baumannii strain A118

45.345

88.095

0.399
  pilT Acinetobacter nosocomialis M2

45.345

88.095

0.399
  pilT Acinetobacter baumannii D1279779

45.345

88.095

0.399
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain ERS1305867

44.444

88.095

0.392
  pilT Legionella pneumophila strain Lp02

44.444

88.095

0.392
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.169

87.831

0.37
  pilT Vibrio cholerae strain A1552

42.169

87.831

0.37