Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EUCAMar_RS12535 Genome accession   NZ_CP132155
Coordinates   2561484..2562533 (-) Length   349 a.a.
NCBI ID   WP_013382398.1    Uniprot ID   A0A6N7WRK3
Organism   Eubacterium callanderi strain DSM 3468     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2556484..2567533
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EUCAMar_RS12525 (EUCAMar_25030) - 2557008..2558288 (-) 1281 WP_013382396.1 O-antigen ligase -
  EUCAMar_RS12530 (EUCAMar_25040) - 2558349..2561465 (-) 3117 WP_013382397.1 EAL domain-containing protein -
  EUCAMar_RS12535 (EUCAMar_25050) pilT 2561484..2562533 (-) 1050 WP_013382398.1 type IV pilus twitching motility protein PilT Machinery gene
  EUCAMar_RS12540 (EUCAMar_25060) - 2562554..2564230 (-) 1677 WP_013382399.1 GspE/PulE family protein -
  EUCAMar_RS12545 (EUCAMar_25070) - 2564350..2564865 (-) 516 WP_013382400.1 type 1 glutamine amidotransferase domain-containing protein -
  EUCAMar_RS12550 (EUCAMar_25080) - 2564867..2566717 (-) 1851 WP_013382401.1 diguanylate cyclase domain-containing protein -
  EUCAMar_RS12555 (EUCAMar_25090) - 2567039..2567461 (+) 423 WP_013382402.1 Hsp20/alpha crystallin family protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38556.07 Da        Isoelectric Point: 6.0014

>NTDB_id=867325 EUCAMar_RS12535 WP_013382398.1 2561484..2562533(-) (pilT) [Eubacterium callanderi strain DSM 3468]
MPTILDLIDYGRQTGCSDIHLTADLPPVFRSNGVLVKGPFEMSKREIGEMIIGMLSEMNREKIKRGEDADFTFVTPEGLR
QRVNVYRQQNELCAAVRLLNDTIPTFEELGLPPIIRKLAAEPRGLILITGPTGSGKSTTLAAMIDYINVNFAKHIITIED
PVEYRHYHKLSMIHQREVGVDVSSFADALRSSLREDPDVILVGEMRDYETISAAVTAAETGHLVLSTLHTIGAANTVDRI
IDVFPPHSQQQIRTQLASTLKGVVTQQLVPLASGEGRMAALEVMTGTDAVLNLIRENKTHQLSSAMQTGSRDGMNTLNSH
LAKLVKEGKIGFDTGLQWSSDKNEFNQYF

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=867325 EUCAMar_RS12535 WP_013382398.1 2561484..2562533(-) (pilT) [Eubacterium callanderi strain DSM 3468]
ATGCCAACCATCTTAGACCTAATCGACTACGGCCGCCAGACTGGCTGTTCGGATATTCATTTGACGGCAGATCTGCCGCC
GGTATTCAGGAGCAACGGGGTGCTTGTCAAAGGCCCCTTTGAAATGAGCAAAAGGGAAATCGGAGAGATGATCATCGGGA
TGCTCAGTGAGATGAACCGTGAGAAAATCAAGCGGGGAGAGGACGCGGATTTTACTTTTGTCACGCCAGAGGGGCTCAGA
CAGAGGGTTAACGTGTACCGCCAGCAGAATGAGCTCTGCGCTGCCGTTCGCCTGCTCAACGACACCATTCCTACCTTTGA
GGAGCTGGGCCTGCCGCCCATTATCCGCAAGCTGGCCGCTGAGCCGAGAGGGCTGATCCTGATCACCGGGCCGACCGGGA
GTGGGAAATCCACCACACTGGCAGCTATGATCGACTACATCAACGTCAATTTTGCCAAGCATATCATCACCATTGAGGAC
CCGGTGGAATACCGCCATTACCATAAACTGAGCATGATCCACCAGCGTGAGGTGGGAGTGGATGTATCCTCCTTTGCCGA
CGCGCTGCGCTCATCCTTGCGTGAGGACCCCGACGTTATTCTGGTAGGGGAAATGCGTGACTACGAAACCATCTCGGCCG
CTGTAACCGCCGCTGAAACCGGGCATCTCGTGCTGTCTACTCTGCACACCATCGGTGCTGCCAACACGGTAGACCGTATT
ATCGACGTGTTCCCGCCCCACAGCCAGCAGCAGATACGGACACAGCTCGCCAGCACCCTGAAGGGTGTTGTGACACAGCA
GCTTGTGCCTCTGGCCTCCGGCGAAGGCCGGATGGCCGCCCTTGAGGTGATGACAGGCACCGACGCAGTGCTCAACCTTA
TCCGTGAAAACAAAACCCATCAGCTTTCCTCTGCCATGCAGACCGGCAGCCGGGACGGCATGAACACCCTGAACAGCCAC
CTGGCCAAGCTGGTGAAGGAAGGAAAGATCGGCTTTGATACCGGCCTCCAGTGGTCGTCAGACAAAAACGAGTTTAACCA
ATACTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N7WRK3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.854

98.281

0.49
  pilT Acinetobacter baylyi ADP1

46.356

98.281

0.456
  pilT Neisseria meningitidis 8013

48.024

94.269

0.453
  pilT Legionella pneumophila strain Lp02

45.507

98.854

0.45
  pilT Legionella pneumophila strain ERS1305867

45.507

98.854

0.45
  pilT Neisseria gonorrhoeae MS11

47.72

94.269

0.45
  pilT Acinetobacter nosocomialis M2

44.898

98.281

0.441
  pilT Acinetobacter baumannii D1279779

44.898

98.281

0.441
  pilT Acinetobacter baumannii strain A118

44.898

98.281

0.441
  pilT Pseudomonas aeruginosa PAK

46.364

94.556

0.438
  pilT Pseudomonas stutzeri DSM 10701

44.606

98.281

0.438
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.732

93.983

0.43
  pilT Vibrio cholerae strain A1552

45.732

93.983

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.896

95.989

0.393