Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q4S45_RS01365 Genome accession   NZ_CP131935
Coordinates   297059..298231 (+) Length   390 a.a.
NCBI ID   WP_305508433.1    Uniprot ID   A0AAT9WGZ6
Organism   Massilia sp. R2A-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 292059..303231
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q4S45_RS01325 (Q4S45_01325) proC 292350..293168 (+) 819 WP_305508417.1 pyrroline-5-carboxylate reductase -
  Q4S45_RS01330 (Q4S45_01330) - 293252..293500 (-) 249 WP_305508419.1 hypothetical protein -
  Q4S45_RS01335 (Q4S45_01335) - 293487..293870 (-) 384 WP_305508421.1 phage holin family protein -
  Q4S45_RS01340 (Q4S45_01340) - 293874..294197 (-) 324 WP_305508423.1 YqjD family protein -
  Q4S45_RS01345 (Q4S45_01345) ubiA 294257..295111 (-) 855 WP_305508425.1 4-hydroxybenzoate octaprenyltransferase -
  Q4S45_RS01350 (Q4S45_01350) - 295185..295517 (-) 333 WP_305508427.1 nucleotidyltransferase family protein -
  Q4S45_RS01355 (Q4S45_01355) - 295517..295939 (-) 423 WP_305508429.1 nucleotidyltransferase substrate binding protein -
  Q4S45_RS01360 (Q4S45_01360) - 295956..296900 (-) 945 WP_305508431.1 hydrogen peroxide-inducible genes activator -
  Q4S45_RS01365 (Q4S45_01365) pilU 297059..298231 (+) 1173 WP_305508433.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q4S45_RS01370 (Q4S45_01370) - 298232..298474 (+) 243 WP_305508435.1 DUF3717 domain-containing protein -
  Q4S45_RS01375 (Q4S45_01375) - 298525..299004 (+) 480 WP_305508437.1 adenylyltransferase/cytidyltransferase family protein -
  Q4S45_RS01380 (Q4S45_01380) - 299066..302419 (-) 3354 WP_305508439.1 patatin-like phospholipase family protein -
  Q4S45_RS01385 (Q4S45_01385) trmL 302575..303045 (-) 471 WP_305508441.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -

Sequence

Protein


Download         Length: 390 a.a.        Molecular weight: 43387.70 Da        Isoelectric Point: 6.3184

>NTDB_id=866371 Q4S45_RS01365 WP_305508433.1 297059..298231(+) (pilU) [Massilia sp. R2A-15]
MSTTFGPAEAQAYIHKLLTVMHHQGGSDLFVSADFPPSMKHQGAMKPLSQQKLSGEVTRALALSLMNDKQKAEFEAEMEC
NFAISLPNVCRFRVNVFVQQQCVAMVVRTIASEIPNFEKLDLPEVLKDVIMTKRGLVLVVGGTGSGKSTTLAAMIDYRNA
NSAGHIITVEDPVEYVHKNKGCLITHREVGVDTHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNSNQTMDRIINFFPEERRSQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTATIGEMILKGNFQGIKEIMHKS
RELGMCTFDQALYELYNKGYIGYDEAIRNADSANGLRLQIKLRGDRKEPEMAGKTTSDDLSMLVEDEPEE

Nucleotide


Download         Length: 1173 bp        

>NTDB_id=866371 Q4S45_RS01365 WP_305508433.1 297059..298231(+) (pilU) [Massilia sp. R2A-15]
ATGTCCACCACTTTTGGGCCCGCCGAAGCGCAGGCGTATATCCACAAACTGCTCACCGTGATGCATCACCAGGGCGGCTC
CGACCTGTTCGTGTCGGCCGATTTCCCGCCGTCGATGAAGCACCAGGGAGCGATGAAGCCGCTGAGCCAACAGAAGCTGT
CGGGCGAAGTCACGCGCGCGCTGGCGCTGTCGCTGATGAACGACAAGCAGAAGGCCGAATTCGAAGCCGAGATGGAGTGC
AATTTCGCCATCTCGCTGCCCAACGTGTGCCGCTTCCGCGTCAACGTCTTCGTCCAGCAGCAGTGCGTGGCGATGGTGGT
GCGCACGATCGCCTCCGAAATCCCGAACTTCGAGAAGCTCGACCTGCCGGAGGTGCTCAAGGACGTCATCATGACCAAGC
GCGGCCTGGTGCTGGTCGTCGGCGGCACCGGCTCGGGCAAGTCGACCACGCTGGCAGCGATGATCGACTACCGCAACGCC
AATTCGGCCGGCCACATCATCACGGTGGAAGACCCGGTCGAATACGTCCACAAGAACAAGGGCTGCCTGATCACGCACCG
CGAAGTCGGCGTGGACACCCACTCGTGGCACAACGCGCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATCG
GCGAGATCCGCGACACCGAAACCATGGAGCACGCGATCGCGTTCGCCGAGACGGGCCACCTGTGCCTTGGCACGCTGCAC
GCGAATAACTCGAACCAGACCATGGACCGGATCATCAACTTCTTCCCGGAAGAGCGGCGCAGCCAACTGCTGATGGACCT
GTCGTCGAACCTGCGCGCGATCGTCTCGCAGCGCCTGGTGCGCACCGAGGACGGCAAGGGCCGCAAGGCGGCGATCGAGA
TCCTGCTGAACACGGCGACGATCGGCGAGATGATCCTGAAGGGGAACTTCCAGGGCATCAAGGAGATCATGCACAAGTCG
CGCGAGCTGGGCATGTGCACGTTTGACCAGGCGCTGTACGAGCTGTATAACAAAGGCTACATCGGCTACGACGAGGCGAT
CCGCAACGCCGACTCGGCCAACGGCCTGCGCCTGCAGATCAAGCTGCGCGGCGACCGCAAGGAGCCGGAAATGGCCGGCA
AGACGACGTCGGACGACCTGTCGATGCTGGTGGAGGACGAGCCGGAGGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.501

88.974

0.521
  pilU Acinetobacter baylyi ADP1

51.213

95.128

0.487
  pilU Vibrio cholerae strain A1552

49.441

91.795

0.454
  pilT Legionella pneumophila strain ERS1305867

43.769

84.359

0.369
  pilT Legionella pneumophila strain Lp02

43.769

84.359

0.369
  pilT Pseudomonas aeruginosa PAK

42.433

86.41

0.367
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.433

86.41

0.367
  pilT Vibrio cholerae strain A1552

43.072

85.128

0.367
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.072

85.128

0.367
  pilT Acinetobacter baylyi ADP1

42.643

85.385

0.364
  pilT Pseudomonas stutzeri DSM 10701

42.136

86.41

0.364
  pilT Acinetobacter baumannii D1279779

42.988

84.103

0.362
  pilT Acinetobacter baumannii strain A118

42.988

84.103

0.362
  pilT Acinetobacter nosocomialis M2

42.988

84.103

0.362