Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   K5638_RS02290 Genome accession   NZ_AP024614
Coordinates   486484..486918 (-) Length   144 a.a.
NCBI ID   WP_144128766.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17382     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481484..491918
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5638_RS02270 (TUM17382_04330) coaE 481738..482349 (-) 612 WP_144133415.1 dephospho-CoA kinase -
  K5638_RS02275 (TUM17382_04340) pilD 482350..483264 (-) 915 WP_221060509.1 A24 family peptidase Machinery gene
  K5638_RS02280 (TUM17382_04350) pilC 483335..484603 (-) 1269 WP_221060510.1 type II secretion system F family protein Machinery gene
  K5638_RS02285 (TUM17382_04360) pilB 484759..486465 (-) 1707 WP_221060511.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5638_RS02290 (TUM17382_04370) pilA/pilAI 486484..486918 (-) 435 WP_144128766.1 pilin Machinery gene
  K5638_RS02295 (TUM17382_04390) nadC 487786..488646 (-) 861 WP_221060512.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5638_RS02300 (TUM17382_04400) ampD 488805..489383 (+) 579 WP_221060513.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5638_RS02305 (TUM17382_04410) ampE 489437..490288 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  K5638_RS02310 (TUM17382_04420) pdhR 490552..491304 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15008.15 Da        Isoelectric Point: 8.4700

>NTDB_id=86574 K5638_RS02290 WP_144128766.1 486484..486918(-) (pilA/pilAI) [Shewanella algae strain TUM17382]
MKGIKLNKRAQGFTLIELMIVVAIIGILAAIALPAYQDYTVKSQVGGAYSEVTSVKSQFEVAKNLGKTPSVTDSDEGYVG
QTAAGGTYCTLAVTATTIVCTAKGGNPDKFNGKTITLTRSAEGVWSCTTNLDAKFRPGDCTASS

Nucleotide


Download         Length: 435 bp        

>NTDB_id=86574 K5638_RS02290 WP_144128766.1 486484..486918(-) (pilA/pilAI) [Shewanella algae strain TUM17382]
ATGAAAGGTATCAAACTTAACAAACGCGCTCAGGGTTTCACCCTGATCGAACTGATGATCGTAGTAGCCATTATCGGTAT
TCTCGCGGCAATCGCACTGCCTGCTTATCAGGATTACACGGTAAAATCACAGGTAGGTGGCGCATACAGTGAAGTAACCA
GTGTTAAGAGCCAATTTGAAGTTGCTAAAAATCTAGGTAAAACACCTTCCGTGACAGATTCTGATGAAGGATACGTTGGA
CAGACTGCCGCTGGAGGCACTTACTGCACCCTGGCTGTCACCGCAACAACTATCGTTTGTACAGCAAAAGGTGGTAACCC
AGATAAGTTTAATGGTAAAACAATTACTCTTACACGATCTGCTGAAGGTGTCTGGAGTTGCACAACTAATTTGGACGCGA
AGTTCAGACCAGGCGATTGCACAGCATCATCTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(8-33)

Pilin (bacterial filament) (Pilin)

(41-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

61.194

93.056

0.569
  pilA/pilAII Pseudomonas stutzeri DSM 10701

53.147

99.306

0.528
  pilA Acinetobacter baumannii strain A118

49.635

95.139

0.472
  pilA Ralstonia pseudosolanacearum GMI1000

38.824

100

0.458
  pilA Vibrio cholerae strain A1552

38.608

100

0.424
  pilA Vibrio cholerae C6706

38.608

100

0.424
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.608

100

0.424
  pilA Pseudomonas aeruginosa PAK

41.216

100

0.424
  pilA2 Legionella pneumophila str. Paris

43.262

97.917

0.424
  pilA2 Legionella pneumophila strain ERS1305867

41.429

97.222

0.403
  comP Acinetobacter baylyi ADP1

40.278

100

0.403
  pilA/pilA1 Eikenella corrodens VA1

39.568

96.528

0.382

Multiple sequence alignment