Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   K5638_RS02285 Genome accession   NZ_AP024614
Coordinates   484759..486465 (-) Length   568 a.a.
NCBI ID   WP_221060511.1    Uniprot ID   -
Organism   Shewanella algae strain TUM17382     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 479759..491465
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5638_RS22525 (TUM17382_04290) - 480000..480272 (-) 273 Protein_418 transposase -
  K5638_RS02255 (TUM17382_04300) mutT 480346..480744 (-) 399 WP_144128772.1 8-oxo-dGTP diphosphatase MutT -
  K5638_RS02260 (TUM17382_04310) yacG 480744..480956 (-) 213 WP_025886789.1 DNA gyrase inhibitor YacG -
  K5638_RS02265 (TUM17382_04320) zapD 481001..481741 (-) 741 WP_028779562.1 cell division protein ZapD -
  K5638_RS02270 (TUM17382_04330) coaE 481738..482349 (-) 612 WP_144133415.1 dephospho-CoA kinase -
  K5638_RS02275 (TUM17382_04340) pilD 482350..483264 (-) 915 WP_221060509.1 A24 family peptidase Machinery gene
  K5638_RS02280 (TUM17382_04350) pilC 483335..484603 (-) 1269 WP_221060510.1 type II secretion system F family protein Machinery gene
  K5638_RS02285 (TUM17382_04360) pilB 484759..486465 (-) 1707 WP_221060511.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K5638_RS02290 (TUM17382_04370) pilA/pilAI 486484..486918 (-) 435 WP_144128766.1 pilin Machinery gene
  K5638_RS02295 (TUM17382_04390) nadC 487786..488646 (-) 861 WP_221060512.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K5638_RS02300 (TUM17382_04400) ampD 488805..489383 (+) 579 WP_221060513.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K5638_RS02305 (TUM17382_04410) ampE 489437..490288 (+) 852 WP_025012050.1 beta-lactamase regulator AmpE -
  K5638_RS02310 (TUM17382_04420) pdhR 490552..491304 (+) 753 WP_025886793.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 63047.14 Da        Isoelectric Point: 5.2550

>NTDB_id=86573 K5638_RS02285 WP_221060511.1 484759..486465(-) (pilB) [Shewanella algae strain TUM17382]
MPNSGLHLGLSTLFIRKGLLDESSLSEAIKQSQKNKQTLVTTLVTSQLLSARTIAELCYEEYGTPLLDLNEFDLSSIPEE
FLNKKLIDKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKVLEEDLSSLDLNGLDEES
LAGIEVTDTDKRQQEEGESKDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLSGRISAR
LKVMSKLDIAERRVPQDGRIKMRLSRNKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEEDQKQAYLEMLARPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSSAETLTRLLNMGVPGYNIASSVNLIIAQRLARRLCPECRQPEDIPEHELLQLGFS
QQQIDAGFTPYKPCGCEFCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIAATAKQQGMRDLRLSGLLKVIQGVTSIA
EINRVTSY

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=86573 K5638_RS02285 WP_221060511.1 484759..486465(-) (pilB) [Shewanella algae strain TUM17382]
ATGCCTAACTCAGGATTGCATTTAGGACTGTCAACGCTATTTATCCGTAAAGGGCTACTCGATGAGAGTAGCCTGTCGGA
GGCTATAAAACAGTCGCAAAAGAATAAACAAACACTGGTCACCACTCTGGTGACCAGTCAGCTTTTATCCGCTCGTACTA
TCGCCGAACTCTGCTACGAAGAGTATGGCACCCCACTGCTGGATCTCAATGAATTTGATTTAAGCAGTATCCCGGAAGAG
TTTCTCAATAAAAAGCTAATCGACAAGCATAGATGCCTGCCACTATTCAAACGCGGCAACCGTCTCTATATCGCCACTTC
AGACCCCACCAATATCGCTGCCCTGGAAGATTTTCAGTTCAGCGCCGGCCTACACGCCGAAGCGATACTGGTAGAGGAGG
ATAAGCTCAGTAAAGCGCTGGAAAAAGTGCTTGAAGAGGACTTGAGTTCACTGGATCTCAATGGCCTCGATGAGGAATCC
CTCGCCGGTATCGAGGTCACCGACACCGACAAGCGTCAACAGGAAGAGGGTGAAAGTAAAGACGATGCGCCTATCGTCAT
CTATATCAATAAGATTTTGACCGATGCTATCCGCAAGGGCGCATCGGATCTGCATTTCGAGCCTTACGAGAAGCGCTACC
GCATCCGTTTTCGTATCGATGGCATCCTCCACGAGGTTTCCGAGCCCCCCATTTCTCTCTCGGGCCGTATCTCTGCCCGC
TTGAAGGTGATGTCCAAACTGGATATCGCCGAGCGCCGGGTACCTCAGGATGGCCGCATCAAGATGAGGCTGTCCCGTAA
CAAGAGTATCGACTTTCGGGTCAGTACCCTGCCCACCCTTTGGGGCGAAAAAATAGTAATGCGGATCCTCGACTCCTCTT
CGGCGCAATTAGGCATCGAAAAACTCGGCTATGAGGAAGACCAAAAACAAGCCTATCTGGAGATGCTGGCAAGGCCCCAG
GGGATGATTCTGGTAACCGGCCCTACCGGCTCTGGTAAAACTGTGTCCCTTTATACTGGCCTCAATATACTCAATACCGA
AGAGCGCAATATCAGCACCGCCGAAGACCCGGTAGAAATAAACCTGGAAGGGGTCAACCAGGTACATATCAACCCCAAGG
CCGGGCTAACCTTCGCCTCAGCGCTGCGCTCCTTTTTGCGTCAGGATCCGGATGTGGTGATGGTGGGGGAAATTCGCGAC
CTAGAAACCGCCGAAATCGCCATCAAGGCGGCGCAAACCGGCCACTTGGTACTCTCAACCCTGCACACCAACTCATCGGC
CGAGACTCTCACCCGCCTGCTCAACATGGGGGTACCAGGTTACAACATTGCCAGTTCGGTCAATCTGATTATTGCCCAGC
GCCTGGCCCGCCGCCTCTGCCCCGAATGCCGTCAACCGGAAGATATCCCCGAGCATGAACTGCTGCAACTGGGCTTCAGT
CAGCAGCAAATAGATGCAGGTTTTACCCCATATAAGCCCTGCGGCTGCGAGTTCTGCTCCGGTGGCTATAAGGGTCGGGT
CGGTATCTATGAGGTAATGAAGATGTCCGATGAAATCGCCAGAACCATTATGGAGGGCGGCAATTCGCTGCAGATTGCCG
CTACCGCCAAACAACAAGGGATGCGTGATTTGCGCCTTTCCGGCCTTTTAAAAGTGATTCAAGGCGTCACCAGTATCGCC
GAGATAAATCGGGTCACCAGCTACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-145)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.916

100

0.593
  pilB Acinetobacter baylyi ADP1

59.358

98.768

0.586
  pilB Legionella pneumophila strain ERS1305867

56.184

99.648

0.56
  pilB Vibrio parahaemolyticus RIMD 2210633

53.086

99.824

0.53
  pilB Vibrio cholerae strain A1552

55.258

92.077

0.509
  pilB Vibrio campbellii strain DS40M4

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

47.788

99.472

0.475
  pilF Thermus thermophilus HB27

38.121

99.296

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.42

98.063

0.377

Multiple sequence alignment