Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   Q7574_RS01665 Genome accession   NZ_CP131867
Coordinates   352197..352631 (+) Length   144 a.a.
NCBI ID   WP_151282585.1    Uniprot ID   -
Organism   Acinetobacter pittii strain 2012N08-034     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 347197..357631
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7574_RS01645 (Q7574_01645) - 348583..349470 (+) 888 WP_014207824.1 metal-dependent hydrolase -
  Q7574_RS01650 (Q7574_01650) - 349601..350191 (+) 591 WP_002117090.1 LemA family protein -
  Q7574_RS01655 (Q7574_01655) - 350216..351295 (+) 1080 WP_342063567.1 TPM domain-containing protein -
  Q7574_RS01660 (Q7574_01660) - 351289..351849 (+) 561 WP_068563948.1 TPM domain-containing protein -
  Q7574_RS01665 (Q7574_01665) pilA 352197..352631 (+) 435 WP_151282585.1 pilin Machinery gene
  Q7574_RS01670 (Q7574_01670) - 352710..354350 (+) 1641 WP_342063568.1 Wzy polymerase domain-containing protein -
  Q7574_RS01675 (Q7574_01675) - 354623..356251 (+) 1629 WP_342063569.1 Wzy polymerase domain-containing protein -
  Q7574_RS01680 (Q7574_01680) bfr 356295..356759 (-) 465 WP_005067113.1 bacterioferritin -
  Q7574_RS01685 (Q7574_01685) bfd 357003..357197 (-) 195 WP_000289091.1 bacterioferritin-associated ferredoxin -
  Q7574_RS01690 (Q7574_01690) - 357300..357428 (+) 129 WP_014207817.1 hypothetical protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14538.70 Da        Isoelectric Point: 8.5025

>NTDB_id=865618 Q7574_RS01665 WP_151282585.1 352197..352631(+) (pilA) [Acinetobacter pittii strain 2012N08-034]
MNAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIARSQVAAGLAEISPAKTNAEAKLAEGISGSITTAAGLGVQASTKRC
SAITVSVSGTDGASTITCALLGTSAIQTKTITWTRTADNPTSGAIGSWSCSTSVAAEFKPKTCQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=865618 Q7574_RS01665 WP_151282585.1 352197..352631(+) (pilA) [Acinetobacter pittii strain 2012N08-034]
ATGAATGCACAAAAAGGTTTTACTTTAATTGAACTCATGATCGTAGTTGCCATTATTGGTATTTTGGCTGCAATTGCGAT
TCCTGCTTATCAAAACTATATTGCTCGTTCGCAAGTTGCAGCTGGACTCGCAGAAATTAGTCCTGCTAAAACGAATGCGG
AAGCAAAATTAGCAGAAGGTATTTCTGGATCAATTACCACTGCAGCAGGATTAGGTGTTCAAGCCTCAACCAAACGCTGT
TCTGCTATTACAGTTAGTGTAAGTGGTACAGACGGTGCAAGTACAATTACATGTGCATTGTTAGGTACATCAGCGATTCA
GACTAAAACAATTACTTGGACTCGTACAGCAGACAACCCAACATCAGGTGCTATTGGTAGCTGGAGTTGTTCTACATCAG
TAGCTGCTGAATTTAAACCAAAAACTTGTCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.694

100

0.507
  pilA Pseudomonas aeruginosa PAK

46.753

100

0.5
  pilA/pilAI Pseudomonas stutzeri DSM 10701

49.306

100

0.493
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

45.033

100

0.472
  pilA Vibrio cholerae strain A1552

45.033

100

0.472
  pilA Vibrio cholerae C6706

45.033

100

0.472
  comP Acinetobacter baylyi ADP1

40.506

100

0.444
  pilA/pilA1 Eikenella corrodens VA1

40.385

100

0.438
  pilA Ralstonia pseudosolanacearum GMI1000

38.854

100

0.424
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.657

99.306

0.424
  pilA Vibrio parahaemolyticus RIMD 2210633

45.113

92.361

0.417
  pilA2 Legionella pneumophila str. Paris

41.379

100

0.417
  pilA2 Legionella pneumophila strain ERS1305867

41.379

100

0.417
  pilE Neisseria gonorrhoeae strain FA1090

35.976

100

0.41