Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LYSHEL_RS04530 Genome accession   NZ_AP024546
Coordinates   912187..913299 (+) Length   370 a.a.
NCBI ID   WP_213436110.1    Uniprot ID   -
Organism   Lysobacter helvus strain JCM32364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 907187..918299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LYSHEL_RS04510 (LYSHEL_08840) - 907465..908634 (-) 1170 WP_213436104.1 cystathionine gamma-synthase -
  LYSHEL_RS04515 (LYSHEL_08850) - 908705..910087 (-) 1383 WP_213436106.1 pyridoxal-phosphate dependent enzyme -
  LYSHEL_RS04520 (LYSHEL_08860) - 910243..910458 (-) 216 WP_213436108.1 YdcH family protein -
  LYSHEL_RS04525 (LYSHEL_08880) - 910777..912030 (-) 1254 WP_244858665.1 OmpA family protein -
  LYSHEL_RS04530 (LYSHEL_08890) pilU 912187..913299 (+) 1113 WP_213436110.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LYSHEL_RS04535 (LYSHEL_08900) maiA 913325..913990 (-) 666 WP_213436112.1 maleylacetoacetate isomerase -
  LYSHEL_RS04540 (LYSHEL_08910) - 914064..915056 (-) 993 WP_213436119.1 fumarylacetoacetate hydrolase family protein -
  LYSHEL_RS04550 (LYSHEL_08920) rpsI 915321..915713 (-) 393 WP_213436121.1 30S ribosomal protein S9 -
  LYSHEL_RS04555 (LYSHEL_08930) rplM 915723..916151 (-) 429 WP_213436123.1 50S ribosomal protein L13 -
  LYSHEL_RS04560 (LYSHEL_08940) - 916300..916830 (-) 531 WP_213436125.1 hypothetical protein -
  LYSHEL_RS04565 (LYSHEL_08950) - 916844..917281 (-) 438 WP_213436127.1 EF-hand domain-containing protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40847.22 Da        Isoelectric Point: 6.2900

>NTDB_id=85854 LYSHEL_RS04530 WP_213436110.1 912187..913299(+) (pilU) [Lysobacter helvus strain JCM32364]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKVEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAIALADSGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKEIIMAPRGLVLIVGSTGSGKSTTLASMIDHRNSTSSGHILTIED
PIEYLHKHKKSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHICLATLHSNNADQTIERI
LNFFPEAAHKNVLMNLALNLKAVVSQRLVVGVDGRRMPAAEVLINTPMIRDLLRRGQVHEIKQAMEESLEAGMESFDQCL
FRLYKEGRIEKEAALQAADSRDGLALKFRLSEGGTGEHDPYADVFETKIG

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=85854 LYSHEL_RS04530 WP_213436110.1 912187..913299(+) (pilU) [Lysobacter helvus strain JCM32364]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAGAAGAACGCGTCGGACATGTTCCTGACGACCGGTGCTCCCGTTTA
CATCAAGGTCGAAGGCAAGTTGTACCCCCTCGGCAACACCGGCCTGCCCCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCGACCTGGAACTGAACATGGCGATCGCGCTGGCCGATTCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCCATCGA
AGAGCTGCAGCTGCCGCAGGTGCTGAAGGAAATCATCATGGCCCCGCGCGGCCTGGTGCTGATCGTCGGCTCCACCGGTT
CGGGCAAGTCGACCACGCTCGCCTCGATGATCGACCACCGCAACAGCACCTCGTCGGGCCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGAAGTCGATCGTCAACCAGCGCGAAGTCGGCCTGGACACGCACGCCTTCCACAA
CGCGCTCAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTGGACGCCACCACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCACATCTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACGATCGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTCAACCTCAAGGCGGTGGTGTCGCAGCG
CCTGGTGGTCGGCGTGGATGGCCGCCGCATGCCGGCCGCCGAAGTGCTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGGCAGGTGCACGAGATCAAGCAGGCGATGGAAGAGTCGCTGGAAGCGGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGCCTCTACAAGGAAGGCCGCATCGAGAAGGAAGCCGCGCTGCAGGCCGCCGACTCGCGCGATGGCCTTGCGCTGAA
GTTCCGCCTCTCCGAGGGCGGCACCGGCGAACACGATCCCTACGCGGACGTGTTCGAAACCAAGATCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

97.027

0.505
  pilU Acinetobacter baylyi ADP1

49.435

95.676

0.473
  pilU Vibrio cholerae strain A1552

48.305

95.676

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.313

91.622

0.424
  pilT Pseudomonas aeruginosa PAK

41.716

91.351

0.381
  pilT Legionella pneumophila strain ERS1305867

41.42

91.351

0.378
  pilT Legionella pneumophila strain Lp02

41.42

91.351

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.124

91.351

0.376
  pilT Acinetobacter baylyi ADP1

40.588

91.892

0.373
  pilT Acinetobacter baumannii D1279779

40.828

91.351

0.373
  pilT Acinetobacter nosocomialis M2

40.828

91.351

0.373
  pilT Acinetobacter baumannii strain A118

40.828

91.351

0.373
  pilT Neisseria gonorrhoeae MS11

38.905

93.784

0.365
  pilT Neisseria meningitidis 8013

38.617

93.784

0.362

Multiple sequence alignment