Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QY917_RS15940 Genome accession   NZ_CP129980
Coordinates   3374172..3375308 (-) Length   378 a.a.
NCBI ID   WP_123674869.1    Uniprot ID   A0AAX1WYJ0
Organism   Diaphorobacter sp. C33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3369172..3380308
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QY917_RS15910 (QY917_15910) - 3369286..3370215 (+) 930 WP_123674866.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  QY917_RS15915 (QY917_15915) rsmI 3370421..3371359 (-) 939 WP_162844514.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  QY917_RS15920 (QY917_15920) - 3371359..3371757 (+) 399 WP_123674867.1 YraN family protein -
  QY917_RS15925 (QY917_15925) - 3371816..3372409 (+) 594 WP_011803876.1 SIS domain-containing protein -
  QY917_RS15930 (QY917_15930) - 3372406..3373080 (+) 675 WP_012655453.1 BON domain-containing protein -
  QY917_RS15935 (QY917_15935) - 3373178..3374107 (-) 930 WP_123674868.1 NAD(P)-dependent oxidoreductase -
  QY917_RS15940 (QY917_15940) pilU 3374172..3375308 (-) 1137 WP_123674869.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QY917_RS15945 (QY917_15945) - 3375355..3375984 (-) 630 WP_047350275.1 Crp/Fnr family transcriptional regulator -
  QY917_RS15950 (QY917_15950) pilT 3376061..3377104 (-) 1044 WP_011803881.1 type IV pilus twitching motility protein PilT Machinery gene
  QY917_RS15955 (QY917_15955) - 3377144..3377860 (+) 717 WP_123674870.1 YggS family pyridoxal phosphate-dependent enzyme -
  QY917_RS15960 (QY917_15960) - 3377899..3379035 (+) 1137 WP_012655458.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41713.23 Da        Isoelectric Point: 7.1529

>NTDB_id=856835 QY917_RS15940 WP_123674869.1 3374172..3375308(-) (pilU) [Diaphorobacter sp. C33]
MERDQASKFINDLLKLMVSRGGSDLFITAEFPPAIKVDGKVTKVSPQPLTPNHTLTLARAIMSDKQVADFERTKECNFAI
SPAGVGRFRVNAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMLDWRNENSFG
HIITVEDPVEFVHPHKNCVITQREVGLDTVSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLKALVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQALFDLFEANVISYEDALRNADSVNDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=856835 QY917_RS15940 WP_123674869.1 3374172..3375308(-) (pilU) [Diaphorobacter sp. C33]
ATGGAACGGGATCAGGCGAGTAAATTCATCAACGATCTGCTCAAGCTGATGGTCAGCCGCGGGGGGAGCGACCTTTTCAT
CACGGCGGAGTTTCCGCCGGCGATCAAGGTCGACGGCAAGGTCACCAAGGTCTCTCCCCAGCCTCTGACGCCCAACCACA
CGCTCACGCTGGCGCGCGCCATCATGAGCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAACTTTGCCATC
TCGCCTGCGGGCGTGGGCCGGTTCCGCGTGAACGCCTTCATACAGCAGGGCCGCGTGGGCATGGTGCTGCGGACGATTCC
GCTCACGCTGCCCACCATCGACGGCCTGGGCGTTCCGCAGGTGCTCAAGGAAGTCACCATGACCAAGCGCGGACTGTGCA
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGCTGGACTGGCGCAACGAGAACTCCTTCGGC
CACATCATTACGGTGGAAGACCCGGTGGAATTCGTGCATCCGCACAAGAACTGCGTGATCACGCAGCGCGAGGTGGGCCT
GGACACCGTCAGCTGGGAGGCGGCCCTCAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAGCACGCCGTGGCGTTTGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCACTGGACCGGATCATCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGTTGATGGACCTGTCGCTGAACCT
CAAGGCACTGGTCTCGCAGCGCCTGATTCCCAAGCAGGACGGCAAGGGGCGCGCGGCCGCCGTCGAGGTGATGCTGAACA
CGCCGCTGATCGCCGACCTCATCTTCAAGGGCGAGGTGGCCGAGATCAAGGAAATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCACTGTTCGACCTGTTCGAGGCCAACGTGATCAGCTATGAGGATGCCCTGCGCAACGCCGA
CTCGGTGAACGACCTGCGCTTGCAGATCAAGCTCAGCAGCCAGCGTGCCAAGACGACGGACCTGGCCTCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.183

92.328

0.593
  pilU Acinetobacter baylyi ADP1

58.38

94.709

0.553
  pilU Vibrio cholerae strain A1552

52.893

96.032

0.508
  pilT Pseudomonas aeruginosa PAK

45.697

89.153

0.407
  pilT Acinetobacter baumannii strain A118

45.646

88.095

0.402
  pilT Acinetobacter nosocomialis M2

45.646

88.095

0.402
  pilT Acinetobacter baumannii D1279779

45.646

88.095

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.51

89.153

0.397
  pilT Pseudomonas stutzeri DSM 10701

43.917

89.153

0.392
  pilT Legionella pneumophila strain Lp02

44.144

88.095

0.389
  pilT Legionella pneumophila strain ERS1305867

44.144

88.095

0.389
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.867

87.831

0.368
  pilT Vibrio cholerae strain A1552

41.867

87.831

0.368