Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QYQ99_RS14495 Genome accession   NZ_CP129672
Coordinates   3136061..3137197 (-) Length   378 a.a.
NCBI ID   WP_302088809.1    Uniprot ID   -
Organism   Comamonas testosteroni strain MWF001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3131061..3142197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QYQ99_RS14470 (QYQ99_14470) rsmI 3132257..3133180 (-) 924 WP_302088805.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  QYQ99_RS14475 (QYQ99_14475) - 3133180..3133581 (+) 402 WP_302088806.1 YraN family protein -
  QYQ99_RS14480 (QYQ99_14480) - 3133671..3134279 (+) 609 WP_043375490.1 SIS domain-containing protein -
  QYQ99_RS14485 (QYQ99_14485) - 3134276..3134929 (+) 654 WP_302088807.1 BON domain-containing protein -
  QYQ99_RS14490 (QYQ99_14490) - 3134990..3135901 (-) 912 WP_302088808.1 NAD(P)-dependent oxidoreductase -
  QYQ99_RS14495 (QYQ99_14495) pilU 3136061..3137197 (-) 1137 WP_302088809.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QYQ99_RS14500 (QYQ99_14500) pilT 3137321..3138364 (-) 1044 WP_302088810.1 type IV pilus twitching motility protein PilT Machinery gene
  QYQ99_RS14505 (QYQ99_14505) - 3138402..3139124 (+) 723 WP_302088811.1 YggS family pyridoxal phosphate-dependent enzyme -
  QYQ99_RS14510 (QYQ99_14510) - 3139255..3140502 (-) 1248 WP_302088812.1 branched-chain amino acid ABC transporter substrate-binding protein -
  QYQ99_RS14515 (QYQ99_14515) - 3140550..3141344 (-) 795 WP_302088813.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41990.48 Da        Isoelectric Point: 6.7686

>NTDB_id=854856 QYQ99_RS14495 WP_302088809.1 3136061..3137197(-) (pilU) [Comamonas testosteroni strain MWF001]
MERDQASKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGKVTKVSPQPLSPLHTLALARSIMNDRQVADFEHTKECNFAI
SPAGVGRFRVNAFMQQGKVGMVLRTIPTTLPTIDGLGVPQVLKEVTMTKRGLCVLVGATGSGKSTTLAAMVDWRNENSFG
HIITVEDPVEFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDVSLNLRAIISQRLIPKQDSKGRVAAIEVMLNSPLIADLIFKGEVVEIKEIMKKSRNIG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLNSQRARTLDLAAGTEHLTIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=854856 QYQ99_RS14495 WP_302088809.1 3136061..3137197(-) (pilU) [Comamonas testosteroni strain MWF001]
ATGGAACGCGATCAGGCCAGTAAATTCATCAACGATCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCGGAGTTTCCGCCCGCCATCAAGATTGACGGAAAAGTCACCAAGGTCTCGCCCCAGCCGCTGAGCCCGCTGCATA
CGCTGGCCCTGGCGCGCTCCATCATGAACGACAGGCAGGTGGCGGACTTCGAGCACACCAAGGAGTGCAACTTCGCCATC
TCGCCGGCCGGCGTGGGTCGCTTCCGCGTCAATGCCTTCATGCAGCAGGGCAAGGTCGGCATGGTGCTGCGTACCATTCC
CACCACCCTGCCCACGATTGACGGCCTGGGCGTGCCGCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCG
TCCTGGTCGGCGCGACGGGCTCGGGCAAGTCCACCACGCTGGCTGCCATGGTGGACTGGCGCAATGAGAACTCCTTCGGC
CACATCATCACGGTGGAAGACCCCGTGGAATTCGTGCACCCCCACAAGAACTGCGTAGTCACGCAACGCGAGGTGGGGCT
GGATACGGACAGCTGGGAAGCGGCACTCAAGAACACGCTGCGCCAGGCTCCCGATGTGATTTTGATGGGCGAAATCCGCG
ACCGCGAAACCATGGAGCACGCGATTGCGTTCTCTGAAACCGGTCACCTGTGCCTGGCCACACTGCACGCCAACAGTGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGTCGCCCGCAACTGCTGATGGACGTTTCGCTGAATCT
GCGGGCCATCATTTCCCAGCGCCTGATCCCCAAGCAGGACAGCAAGGGCCGTGTAGCGGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGTCGAGATCAAGGAGATCATGAAGAAAAGCCGCAATATCGGC
ATGCAGACCTTTGACCAGGCGCTGTTCGACCTGTTCGAATCCAATCTCATCACCTACGAAGATGCGCTGCGCAATGCAGA
CTCGGTCAACGACCTGCGTCTGCAGATCAAGCTCAACAGCCAGCGCGCCCGGACTCTGGACCTGGCGGCCGGCACCGAGC
ATCTGACTATCGTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.749

96.825

0.598
  pilU Acinetobacter baylyi ADP1

55.989

94.974

0.532
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.73

89.153

0.381
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.862

0.362
  pilT Vibrio cholerae strain A1552

43.218

83.862

0.362
  pilT Acinetobacter baylyi ADP1

41.768

86.772

0.362