Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   GSVR_RS19665 Genome accession   NZ_AP024469
Coordinates   4187256..4188344 (-) Length   362 a.a.
NCBI ID   WP_173195499.1    Uniprot ID   A0A6F9WRP0
Organism   Geobacter sp. SVR     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 4181820..4191694 4187256..4188344 within 0


Gene organization within MGE regions


Location: 4181820..4191694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GSVR_RS19635 (GSVR_39510) - 4181820..4182908 (-) 1089 WP_173195495.1 hypothetical protein -
  GSVR_RS19640 (GSVR_39520) - 4182914..4183873 (-) 960 WP_203978730.1 tyrosine-type recombinase/integrase -
  GSVR_RS19645 (GSVR_39530) - 4184157..4184462 (-) 306 WP_173195496.1 hypothetical protein -
  GSVR_RS19650 (GSVR_39540) - 4184836..4186191 (+) 1356 WP_173195497.1 sigma-54 dependent transcriptional regulator -
  GSVR_RS19655 (GSVR_39550) - 4186232..4186729 (-) 498 WP_173195916.1 molybdenum cofactor biosynthesis protein B -
  GSVR_RS19660 (GSVR_39560) moaC 4186735..4187232 (-) 498 WP_173195498.1 cyclic pyranopterin monophosphate synthase MoaC -
  GSVR_RS19665 (GSVR_39570) pilT 4187256..4188344 (-) 1089 WP_173195499.1 type IV pilus twitching motility protein PilT Machinery gene
  GSVR_RS19670 (GSVR_39580) dnaB 4188577..4189956 (-) 1380 WP_173195500.1 replicative DNA helicase -
  GSVR_RS19675 (GSVR_39590) - 4189972..4191135 (-) 1164 WP_173195501.1 DegT/DnrJ/EryC1/StrS aminotransferase family protein -
  GSVR_RS19680 (GSVR_39600) - 4191171..4191467 (-) 297 WP_173195502.1 type II toxin-antitoxin system RelE/ParE family toxin -
  GSVR_RS19685 (GSVR_39610) - 4191464..4191694 (-) 231 WP_173195503.1 addiction module protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 40078.47 Da        Isoelectric Point: 7.1157

>NTDB_id=85268 GSVR_RS19665 WP_173195499.1 4187256..4188344(-) (pilT) [Geobacter sp. SVR]
MARIDALFKMMKDEGASDLHLSTGNPPIFRLHGEMVRLNFKSLGHEDLKAILFEILSEKQRAQFEASKDLDFAYSVEGLA
RFRGNILMQHRGIAAVFRMIPSKILSAEQLALPEGVLRMTRFKKGLVVVTGPTGSGKSTTLAAMVDLINSTRKEHILTLE
DPLEFIHENKLSLVNQRQIGEHTESFASALRAALREDPDVIMVGEMRDLTTIQLAMSAAETGHLVFGTLHTNTAAKTIDR
IIDVFPTDQQEQVRAMLSESLKGVVCQQLLKTADGRGRVAALEIMLGTPAIANLIREAKTFQIPSIMQTGKKEGMQLMDT
HLMELLKAQRVNPEEAYRCAIDKKQFEQFLPHEAAAPAAGGH

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=85268 GSVR_RS19665 WP_173195499.1 4187256..4188344(-) (pilT) [Geobacter sp. SVR]
ATGGCGCGTATCGATGCGTTGTTCAAGATGATGAAGGACGAGGGTGCCTCGGACCTGCACCTCTCCACCGGCAATCCTCC
GATCTTCCGCCTGCATGGGGAAATGGTGCGCCTCAACTTCAAGTCGCTGGGACATGAGGATCTGAAGGCCATCCTGTTCG
AGATCCTGTCAGAGAAGCAGCGCGCACAGTTCGAGGCCAGCAAGGATCTCGACTTTGCCTATTCCGTGGAGGGGCTGGCC
CGTTTCCGCGGCAACATCCTCATGCAGCACCGTGGCATTGCCGCGGTTTTCCGTATGATCCCCAGCAAAATCCTGTCGGC
GGAGCAGCTGGCACTTCCGGAAGGGGTGCTGCGCATGACCAGGTTCAAGAAGGGACTGGTGGTGGTGACCGGGCCGACCG
GTTCGGGCAAGTCCACCACCCTGGCGGCCATGGTGGACCTGATCAATTCCACCCGCAAGGAACACATCCTGACCCTGGAA
GACCCGCTGGAGTTCATACATGAGAACAAGCTCTCGCTCGTGAACCAGCGCCAGATCGGCGAGCACACCGAGAGCTTCGC
CTCGGCCCTGCGGGCGGCCCTGCGTGAGGACCCGGATGTGATCATGGTGGGGGAAATGCGCGACCTGACCACCATCCAAT
TAGCCATGAGCGCCGCCGAGACCGGCCATCTGGTGTTCGGCACCCTGCACACCAACACGGCCGCCAAGACCATCGACCGT
ATCATCGATGTCTTCCCCACCGATCAGCAGGAACAGGTGCGCGCCATGCTGTCCGAGTCGCTGAAAGGGGTGGTATGCCA
GCAGTTGCTCAAGACCGCCGACGGCAGGGGACGGGTAGCGGCCCTGGAGATTATGCTCGGTACACCGGCCATCGCCAACC
TGATTCGGGAGGCCAAGACCTTCCAGATCCCCTCCATCATGCAGACTGGCAAGAAGGAGGGCATGCAGCTGATGGACACG
CACCTGATGGAACTGCTCAAGGCCCAGCGAGTCAATCCCGAAGAGGCCTACCGCTGTGCCATCGACAAGAAGCAGTTCGA
ACAGTTCCTGCCCCATGAAGCGGCTGCTCCGGCAGCAGGCGGGCATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6F9WRP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

54.624

95.58

0.522
  pilT Legionella pneumophila strain Lp02

56.798

91.436

0.519
  pilT Legionella pneumophila strain ERS1305867

56.798

91.436

0.519
  pilT Acinetobacter nosocomialis M2

53.89

95.856

0.517
  pilT Acinetobacter baumannii D1279779

53.89

95.856

0.517
  pilT Acinetobacter baumannii strain A118

53.89

95.856

0.517
  pilT Pseudomonas aeruginosa PAK

55.255

91.989

0.508
  pilT Acinetobacter baylyi ADP1

54.683

91.436

0.5
  pilT Vibrio cholerae strain A1552

52.616

95.028

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.616

95.028

0.5
  pilT Neisseria meningitidis 8013

54.769

89.779

0.492
  pilT Neisseria gonorrhoeae MS11

54.462

89.779

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.58

98.619

0.489
  pilU Vibrio cholerae strain A1552

40.308

89.779

0.362

Multiple sequence alignment