Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LU655_RS27345 Genome accession   NZ_CP128547
Coordinates   5846481..5847479 (-) Length   332 a.a.
NCBI ID   WP_016712507.1    Uniprot ID   -
Organism   Pseudomonas monteilii strain NMI10873_11     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5841481..5852479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LU655_RS27325 (LU655_027325) argS 5842686..5844422 (+) 1737 WP_016712503.1 arginine--tRNA ligase -
  LU655_RS27330 (LU655_027330) - 5844424..5845122 (+) 699 WP_016712504.1 SPOR domain-containing protein -
  LU655_RS27335 (LU655_027335) - 5845209..5845625 (-) 417 WP_016712505.1 NINE protein -
  LU655_RS27340 (LU655_027340) - 5845793..5846419 (+) 627 WP_016712506.1 C40 family peptidase -
  LU655_RS27345 (LU655_027345) pilT 5846481..5847479 (-) 999 WP_016712507.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LU655_RS27350 (LU655_027350) - 5847545..5848231 (+) 687 WP_016712508.1 YggS family pyridoxal phosphate-dependent enzyme -
  LU655_RS27355 (LU655_027355) proC 5848270..5849088 (+) 819 WP_025752259.1 pyrroline-5-carboxylate reductase -
  LU655_RS27360 (LU655_027360) - 5849099..5849689 (+) 591 WP_016712510.1 YggT family protein -
  LU655_RS27365 (LU655_027365) - 5849778..5850917 (+) 1140 WP_016712511.1 homoserine O-acetyltransferase -
  LU655_RS27370 (LU655_027370) metW 5850925..5851545 (+) 621 WP_003249106.1 methionine biosynthesis protein MetW -
  LU655_RS27375 (LU655_027375) - 5851568..5852002 (+) 435 WP_016712512.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36086.76 Da        Isoelectric Point: 6.3976

>NTDB_id=848737 LU655_RS27345 WP_016712507.1 5846481..5847479(-) (pilT) [Pseudomonas monteilii strain NMI10873_11]
MDVTDLLARAVEAGASDLHLAAGQIPMLRLDGELQRMYLPPLVAADLAEGMALLMTEQQRRQWAEGDELDLALALPTLGR
FRLNLFRQLNGPGATFRLIPGRIATLDELDLGDVYQAVAQCHDGLVLVGGPTGSGKSSTLASLLDQLNRERALHIITLED
PVEVIHSSQRSLVNQREIGRHSGGFAQGLRSALRQDPDVIMIGELRDLETVRLALRAAETGHLVLATVHTRSAVNSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVRRVGGGRVAAREVLVATTAVRNLVREGRMAQLCSVMQAGAAEGMRTMEGAM
RELKEKRLISDL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=848737 LU655_RS27345 WP_016712507.1 5846481..5847479(-) (pilT) [Pseudomonas monteilii strain NMI10873_11]
ATGGATGTGACCGACCTGTTGGCCCGAGCCGTGGAGGCGGGGGCTTCGGACCTGCACCTGGCGGCGGGCCAGATACCGAT
GCTGCGCCTGGACGGTGAATTGCAGCGCATGTACTTGCCGCCGCTGGTGGCGGCAGACCTGGCCGAGGGTATGGCGCTCT
TGATGACAGAGCAGCAAAGGCGGCAATGGGCCGAGGGCGATGAACTGGACTTGGCGCTGGCATTGCCCACGCTCGGCCGT
TTTCGGCTGAACCTGTTTCGCCAGCTGAATGGCCCTGGAGCAACCTTTCGGCTGATCCCCGGGCGGATCGCCACGCTGGA
TGAACTGGACCTGGGGGATGTGTATCAAGCTGTTGCGCAATGCCATGATGGCTTGGTCCTGGTGGGTGGGCCGACCGGCA
GTGGCAAGTCCAGCACCCTGGCGTCGCTGCTTGACCAGCTGAACCGGGAGCGTGCGCTGCACATCATTACCCTGGAAGAC
CCGGTCGAAGTTATCCACAGCAGCCAACGCAGCCTGGTCAATCAGCGTGAGATTGGCCGCCACAGCGGCGGCTTTGCCCA
GGGGCTGCGCAGTGCCCTGCGACAGGACCCGGATGTGATCATGATTGGCGAATTGCGCGACCTGGAGACCGTTCGTCTGG
CCTTGCGCGCGGCAGAGACTGGGCACCTGGTGCTGGCGACGGTGCATACGCGTTCGGCCGTAAACAGCATCGATCGGTTG
GTGGAGGTGTTTGCTGCCGAGGAAAAGCCGTTGGTGCGCGCGATGCTGGCCGAGTCGCTGCGCCTGGTGGTGGCGCAGGT
GCTGGTCAGGCGCGTAGGTGGCGGGCGGGTTGCAGCGCGGGAAGTGCTGGTGGCGACGACGGCGGTGCGTAACCTGGTGC
GTGAGGGGCGGATGGCGCAGTTGTGTTCGGTGATGCAGGCGGGTGCTGCAGAAGGGATGCGGACGATGGAGGGGGCAATG
CGGGAGCTGAAGGAAAAGCGATTGATCAGCGATTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.818

99.398

0.515
  pilT Acinetobacter baumannii D1279779

50.909

99.398

0.506
  pilT Acinetobacter baumannii strain A118

50.909

99.398

0.506
  pilT Acinetobacter baylyi ADP1

50.909

99.398

0.506
  pilT Acinetobacter nosocomialis M2

50.909

99.398

0.506
  pilT Pseudomonas stutzeri DSM 10701

50.606

99.398

0.503
  pilT Legionella pneumophila strain ERS1305867

49.394

99.398

0.491
  pilT Legionella pneumophila strain Lp02

49.394

99.398

0.491
  pilT Vibrio cholerae strain A1552

50.464

97.289

0.491
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.464

97.289

0.491
  pilT Neisseria meningitidis 8013

47.432

99.699

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.432

99.699

0.473
  pilT Neisseria gonorrhoeae MS11

47.13

99.699

0.47
  pilU Pseudomonas stutzeri DSM 10701

38.889

97.59

0.38