Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   QR290_RS25095 Genome accession   NZ_CP128260
Coordinates   5595727..5596149 (-) Length   140 a.a.
NCBI ID   WP_115079240.1    Uniprot ID   A0A345V2M1
Organism   Pseudomonas fluorescens strain PH.SM     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5590727..5601149
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS25080 (QR290_25080) - 5591658..5594609 (+) 2952 WP_289203821.1 DUF748 domain-containing protein -
  QR290_RS25085 (QR290_25085) - 5594609..5594905 (+) 297 WP_289203822.1 DUF2845 domain-containing protein -
  QR290_RS25090 (QR290_25090) - 5595073..5595429 (-) 357 WP_115079239.1 BON domain-containing protein -
  QR290_RS25095 (QR290_25095) pilA/pilAI 5595727..5596149 (-) 423 WP_115079240.1 pilin Machinery gene
  QR290_RS25100 (QR290_25100) pilB 5596376..5598076 (+) 1701 WP_289203823.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QR290_RS25105 (QR290_25105) pilC 5598079..5599296 (+) 1218 WP_289203824.1 type II secretion system F family protein Machinery gene
  QR290_RS25110 (QR290_25110) pilD 5599298..5600170 (+) 873 WP_289203825.1 A24 family peptidase Machinery gene
  QR290_RS25115 (QR290_25115) coaE 5600167..5600790 (+) 624 WP_289203826.1 dephospho-CoA kinase -
  QR290_RS25120 (QR290_25120) yacG 5600787..5600987 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14103.26 Da        Isoelectric Point: 8.4918

>NTDB_id=846923 QR290_RS25095 WP_115079240.1 5595727..5596149(-) (pilA/pilAI) [Pseudomonas fluorescens strain PH.SM]
MKKQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISAMKVPFEDIINQGTNPTLAAVKGDAGTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVVGKTITLTRTAANGWSCATTVAPDYSPKGCTASGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=846923 QR290_RS25095 WP_115079240.1 5595727..5596149(-) (pilA/pilAI) [Pseudomonas fluorescens strain PH.SM]
ATGAAGAAACAACAAGGTTTCACTCTGATCGAGCTGCTGATCGTTGTGGCGATCATCGGGATTCTGGCGACGATTGCCTT
GCCGCAGTACTCCAAATATCAGGCGCGGTCGAAGGTAACGGCGGGGCTGGCCGAGATCTCGGCAATGAAAGTGCCGTTCG
AAGACATCATCAATCAAGGAACGAACCCTACACTGGCCGCGGTTAAGGGCGATGCGGGCACTACCACCTCCAACTGTGCA
CTGACCGCCTCGGGGACGGCTGCAGATGGAGCGGGCACCATCGTTTGTACGCTGGCCAATGCGCCAGCACCCGTAGTGGG
CAAAACCATCACGCTCACCCGTACCGCTGCCAACGGCTGGTCATGTGCAACGACCGTCGCCCCGGACTATTCACCTAAAG
GCTGCACTGCGAGCGGCACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A345V2M1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.825

97.857

0.507
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42.581

100

0.471
  pilA Vibrio cholerae strain A1552

42.581

100

0.471
  pilA Vibrio cholerae C6706

42.581

100

0.471
  pilA Acinetobacter baumannii strain A118

47.482

99.286

0.471
  comP Acinetobacter baylyi ADP1

42.763

100

0.464
  pilA Pseudomonas aeruginosa PAK

40.26

100

0.443
  pilA2 Legionella pneumophila str. Paris

42.857

100

0.429
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

96.429

0.429
  pilA2 Legionella pneumophila strain ERS1305867

42.143

100

0.421
  pilA Vibrio parahaemolyticus RIMD 2210633

42.963

96.429

0.414
  pilA/pilA1 Eikenella corrodens VA1

35.862

100

0.371