Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QR290_RS25100 Genome accession   NZ_CP128260
Coordinates   5596376..5598076 (+) Length   566 a.a.
NCBI ID   WP_289203823.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain PH.SM     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5591376..5603076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QR290_RS25080 (QR290_25080) - 5591658..5594609 (+) 2952 WP_289203821.1 DUF748 domain-containing protein -
  QR290_RS25085 (QR290_25085) - 5594609..5594905 (+) 297 WP_289203822.1 DUF2845 domain-containing protein -
  QR290_RS25090 (QR290_25090) - 5595073..5595429 (-) 357 WP_115079239.1 BON domain-containing protein -
  QR290_RS25095 (QR290_25095) pilA/pilAI 5595727..5596149 (-) 423 WP_115079240.1 pilin Machinery gene
  QR290_RS25100 (QR290_25100) pilB 5596376..5598076 (+) 1701 WP_289203823.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QR290_RS25105 (QR290_25105) pilC 5598079..5599296 (+) 1218 WP_289203824.1 type II secretion system F family protein Machinery gene
  QR290_RS25110 (QR290_25110) pilD 5599298..5600170 (+) 873 WP_289203825.1 A24 family peptidase Machinery gene
  QR290_RS25115 (QR290_25115) coaE 5600167..5600790 (+) 624 WP_289203826.1 dephospho-CoA kinase -
  QR290_RS25120 (QR290_25120) yacG 5600787..5600987 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  QR290_RS25125 (QR290_25125) - 5600994..5601209 (-) 216 WP_007959584.1 hypothetical protein -
  QR290_RS25130 (QR290_25130) - 5601275..5601964 (-) 690 WP_289203827.1 energy-coupling factor ABC transporter permease -
  QR290_RS25135 (QR290_25135) - 5602098..5602724 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62891.02 Da        Isoelectric Point: 5.9468

>NTDB_id=846924 QR290_RS25100 WP_289203823.1 5596376..5598076(+) (pilB) [Pseudomonas fluorescens strain PH.SM]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESVDDSKQDTLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVMRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPASCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDIQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=846924 QR290_RS25100 WP_289203823.1 5596376..5598076(+) (pilB) [Pseudomonas fluorescens strain PH.SM]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAATTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAGATTGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGCCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTTTGGCGACGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AGCTCACCGACGCCATCGAAAAGTTCTTCGACACCCACGCCACCGGCCTGGAGGAGATGGCGGATGTCGACCTCGACGGG
CTGGACATCGAGTCGGTCGACGACAGCAAGCAGGACACACTCGGCGGAATCGACGCGGACGATGCCCCGGTGGTGCGCTT
CGTACACAAGATGCTGCTGGATGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCTTACGAAAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAGGTGGCCAAACCGCCGATTCAACTGGCCGGGCGCATTGCCGCACGCCTG
AAAGTCATGGCCAGTCTGGACATTTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAGAAAGTGGTGATGCGGATCCTCGATCCGTCCAGTG
CCCAGATCGGCATCGATGCCCTCGGTTACGAGCCGGAGCAGAAAGACCTGTACATGGCGGCCCTCAAACAGCCGCAAGGC
ATGATTCTGGTAACGGGGCCCACCGGTTCGGGCAAGACCGTGTCGCTGTATACCGGGCTGAACATCCTCAATACCGTCGA
CATCAACATCTCCACCGCTGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCACGTCAGG
GGATGGACTTTGCCCAGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTC
GAAACCGCCGAAATCGCGATCAAGGCTGCTCAGACCGGGCACCTCGTGCTGTCCACCCTGCACACCAACAGCGCCGCGGA
AACACTGATCCGTCTGCACAACATGGGCATTCCCGGCTTCAATATCGCAACCTCGGTGAGCCTGATCATTGCCCAGCGGC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAACCCATCGAAATCCCTCGCGAAACCCTGATCAAGGAGGGCTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCGCCAGCTGCGATCACTGCAACGGCGGTTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAGAATACTCCCGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATATCCAGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.672

100

0.578
  pilB Acinetobacter baumannii D1279779

57.319

100

0.574
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

49.383

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.438

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilF Thermus thermophilus HB27

36.559

98.587

0.36
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.636

93.286

0.36