Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VZG47_RS07115 Genome accession   NZ_CP144288
Coordinates   1423675..1424781 (-) Length   368 a.a.
NCBI ID   WP_338429265.1    Uniprot ID   -
Organism   Synechococcus elongatus IITB5     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1418675..1429781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VZG47_RS07085 (VZG47_07060) - 1419122..1419445 (+) 324 WP_338429262.1 phasin family protein -
  VZG47_RS07090 (VZG47_07065) - 1419500..1420024 (+) 525 WP_208676181.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  VZG47_RS07095 (VZG47_07070) lipB 1420097..1420834 (-) 738 WP_338431834.1 lipoyl(octanoyl) transferase LipB -
  VZG47_RS07100 (VZG47_07075) raiA 1421040..1421663 (+) 624 WP_208676179.1 ribosome-associated translation inhibitor RaiA -
  VZG47_RS07105 (VZG47_07080) larC 1421720..1422928 (-) 1209 WP_338429263.1 nickel pincer cofactor biosynthesis protein LarC -
  VZG47_RS07110 (VZG47_07085) - 1422944..1423645 (+) 702 WP_338429264.1 L-threonylcarbamoyladenylate synthase -
  VZG47_RS07115 (VZG47_07090) pilT 1423675..1424781 (-) 1107 WP_338429265.1 type IV pilus twitching motility protein PilT Machinery gene
  VZG47_RS07120 (VZG47_07095) queA 1424949..1426028 (-) 1080 WP_338429266.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  VZG47_RS07125 (VZG47_07100) - 1426167..1427123 (+) 957 WP_338438791.1 cupin -
  VZG47_RS07130 (VZG47_07105) - 1427120..1427920 (-) 801 WP_338429268.1 TIGR01548 family HAD-type hydrolase -
  VZG47_RS07135 (VZG47_07110) - 1428338..1428676 (+) 339 WP_208676167.1 30S ribosomal protein PSRP-3 -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41030.99 Da        Isoelectric Point: 5.3135

>NTDB_id=846843 VZG47_RS07115 WP_338429265.1 1423675..1424781(-) (pilT) [Synechococcus elongatus IITB5]
MPAPAPAASPQPAASKQPSLAEIVRQAYEHDYSDVHVGVGEVPRFRDRGEIVQTTYPVTDTATFNGWLREVLTEEQVREF
EQGLDFDGATQYDFARVRINIFGSLRGPSMVLRLIPLRILSLDELSLPPVFRDICYYPKGLVLVTGPTGSGKSTTLAAMI
DFINQEMAKNIITIEDPIEFVHQSQRSLIKQREVGVHTLKFEAALRASLREDPDIILVGELRDRETINTALKAAQTGHLV
MATLHTNSAVKTIERVLNMFEPAEQPPVRIALAESLVAIIAQGLCRTIQGKRAAYHEILINTDAIKDYIIRGELEEVEQL
IPKCTFDGMCTSNQSLFRLFNEGRISEETALSQSSKPNEMSQMLRGRV

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=846843 VZG47_RS07115 WP_338429265.1 1423675..1424781(-) (pilT) [Synechococcus elongatus IITB5]
ATGCCTGCGCCTGCCCCAGCCGCATCTCCTCAGCCTGCGGCCAGTAAGCAGCCGAGCTTGGCAGAAATTGTCCGACAAGC
CTACGAGCACGACTACTCGGATGTGCACGTGGGGGTTGGAGAAGTACCGCGCTTCCGCGATCGCGGCGAGATTGTACAAA
CGACCTATCCGGTTACGGATACCGCCACGTTTAATGGTTGGCTGCGCGAAGTACTGACGGAAGAACAGGTGCGCGAGTTT
GAGCAGGGCTTAGACTTCGACGGCGCAACCCAATACGACTTTGCGCGGGTTCGGATCAATATCTTTGGGTCGCTGCGGGG
CCCTTCCATGGTGTTGCGGTTAATTCCGCTGCGCATTCTCAGCCTCGATGAGTTGAGCCTCCCGCCGGTCTTCCGCGATA
TTTGCTACTACCCCAAAGGACTGGTGCTCGTCACAGGCCCTACGGGATCGGGGAAATCAACGACTCTCGCCGCCATGATT
GACTTCATCAATCAGGAAATGGCGAAAAATATCATCACGATTGAGGATCCCATCGAGTTTGTTCACCAGAGCCAGCGATC
GCTGATTAAGCAACGCGAAGTCGGTGTCCATACGCTGAAGTTTGAGGCGGCTTTGCGAGCTTCGTTGCGGGAAGACCCTG
ACATTATCCTCGTTGGTGAGTTGCGCGATCGCGAAACGATTAATACGGCACTCAAAGCGGCTCAAACAGGCCATTTGGTG
ATGGCAACCCTGCACACCAACAGTGCTGTTAAAACGATTGAGCGGGTGCTCAACATGTTTGAACCCGCTGAGCAGCCACC
TGTGCGGATTGCTCTTGCTGAATCTCTGGTGGCGATTATTGCCCAAGGACTCTGCCGCACTATTCAAGGCAAACGAGCTG
CTTATCACGAGATTTTGATTAACACGGATGCCATTAAGGACTACATCATTCGCGGTGAACTGGAAGAAGTGGAGCAGTTA
ATTCCCAAATGTACTTTTGACGGAATGTGCACTAGTAATCAGTCTCTCTTCCGACTCTTTAATGAAGGCAGAATTAGTGA
GGAGACGGCGCTCTCGCAATCGAGTAAGCCCAATGAAATGTCGCAGATGCTGCGGGGGCGCGTTTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(23-286)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

46.018

92.12

0.424
  pilT Acinetobacter nosocomialis M2

45.133

92.12

0.416
  pilT Acinetobacter baumannii D1279779

45.133

92.12

0.416
  pilT Acinetobacter baumannii strain A118

45.133

92.12

0.416
  pilT Neisseria meningitidis 8013

45.619

89.946

0.41
  pilT Legionella pneumophila strain ERS1305867

45.181

90.217

0.408
  pilT Legionella pneumophila strain Lp02

45.181

90.217

0.408
  pilT Neisseria gonorrhoeae MS11

45.317

89.946

0.408
  pilT Pseudomonas stutzeri DSM 10701

43.953

92.12

0.405
  pilT Pseudomonas aeruginosa PAK

43.658

92.12

0.402
  pilT Vibrio cholerae strain A1552

43.881

91.033

0.399
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.881

91.033

0.399
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.922

94.293

0.386
  pilU Pseudomonas stutzeri DSM 10701

39.429

95.109

0.375