Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QQ016_RS13185 Genome accession   NZ_CP127228
Coordinates   2962113..2962559 (+) Length   148 a.a.
NCBI ID   WP_285962465.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain KRS022     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2957113..2967559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQ016_RS13170 nadC 2959205..2960053 (+) 849 WP_285962463.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QQ016_RS13180 - 2960230..2961957 (-) 1728 WP_285962464.1 Wzy polymerase domain-containing protein -
  QQ016_RS13185 pilA 2962113..2962559 (+) 447 WP_285962465.1 pilin Machinery gene
  QQ016_RS13190 pilB 2962675..2964378 (+) 1704 WP_285962466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QQ016_RS13195 pilC 2964382..2965602 (+) 1221 WP_285962467.1 type II secretion system F family protein Machinery gene
  QQ016_RS13200 pilD 2965603..2966472 (+) 870 WP_285962468.1 A24 family peptidase Machinery gene
  QQ016_RS13205 coaE 2966472..2967083 (+) 612 WP_173173307.1 dephospho-CoA kinase -
  QQ016_RS13210 yacG 2967080..2967280 (+) 201 WP_285962469.1 DNA gyrase inhibitor YacG -
  QQ016_RS13215 - 2967284..2967499 (-) 216 WP_285962470.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15042.11 Da        Isoelectric Point: 9.1607

>NTDB_id=842877 QQ016_RS13185 WP_285962465.1 2962113..2962559(+) (pilA) [Pseudomonas tohonis strain KRS022]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIARSEAASGLAALAPLKTAVEDAYARGASTSDFTLPLLGSAATASPL
GSISFPTSFTAAGDGALQFNFGTNGTSGPRTTGQALRLTRNASGTWTCTSSAGLATELRPRNCATNTP

Nucleotide


Download         Length: 447 bp        

>NTDB_id=842877 QQ016_RS13185 WP_285962465.1 2962113..2962559(+) (pilA) [Pseudomonas tohonis strain KRS022]
ATGAAAGCACAGAAAGGCTTCACCCTGATCGAACTGATGATCGTTGTTGCGATCATCGGCATCCTCGCTGCCATCGCCAT
TCCGGCGTACCAGAACTACATTGCACGTTCTGAAGCAGCCAGCGGCCTGGCCGCTCTTGCTCCGCTCAAGACTGCAGTTG
AGGATGCCTATGCCCGTGGCGCCTCTACTTCTGACTTCACCCTCCCCCTGCTGGGCAGCGCCGCTACTGCAAGCCCCCTG
GGCTCCATCAGCTTCCCCACCAGCTTCACCGCTGCAGGCGACGGTGCCCTGCAGTTCAACTTTGGCACTAACGGCACTTC
CGGCCCCCGCACCACCGGTCAAGCGCTCCGCCTGACCCGCAACGCGAGTGGCACTTGGACCTGCACCAGCAGCGCTGGCC
TTGCCACCGAACTGCGCCCGCGCAACTGCGCGACCAACACCCCGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

52.903

100

0.554
  pilA Acinetobacter baumannii strain A118

46.98

100

0.473
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.384

100

0.432
  pilA Vibrio cholerae C6706

41.06

100

0.419
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.06

100

0.419
  pilA Vibrio cholerae strain A1552

41.06

100

0.419
  pilA Vibrio parahaemolyticus RIMD 2210633

46.565

88.514

0.412
  pilA2 Legionella pneumophila strain ERS1305867

40.541

100

0.405
  pilA2 Legionella pneumophila str. Paris

40.541

100

0.405
  pilA Ralstonia pseudosolanacearum GMI1000

43.478

93.243

0.405
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.288

93.919

0.378