Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QQ016_RS13190 Genome accession   NZ_CP127228
Coordinates   2962675..2964378 (+) Length   567 a.a.
NCBI ID   WP_285962466.1    Uniprot ID   -
Organism   Pseudomonas tohonis strain KRS022     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2957675..2969378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQ016_RS13170 nadC 2959205..2960053 (+) 849 WP_285962463.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QQ016_RS13180 - 2960230..2961957 (-) 1728 WP_285962464.1 Wzy polymerase domain-containing protein -
  QQ016_RS13185 pilA 2962113..2962559 (+) 447 WP_285962465.1 pilin Machinery gene
  QQ016_RS13190 pilB 2962675..2964378 (+) 1704 WP_285962466.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QQ016_RS13195 pilC 2964382..2965602 (+) 1221 WP_285962467.1 type II secretion system F family protein Machinery gene
  QQ016_RS13200 pilD 2965603..2966472 (+) 870 WP_285962468.1 A24 family peptidase Machinery gene
  QQ016_RS13205 coaE 2966472..2967083 (+) 612 WP_173173307.1 dephospho-CoA kinase -
  QQ016_RS13210 yacG 2967080..2967280 (+) 201 WP_285962469.1 DNA gyrase inhibitor YacG -
  QQ016_RS13215 - 2967284..2967499 (-) 216 WP_285962470.1 hypothetical protein -
  QQ016_RS13220 - 2967607..2968293 (-) 687 WP_285962471.1 energy-coupling factor ABC transporter permease -
  QQ016_RS13225 - 2968290..2968757 (-) 468 WP_285962472.1 FAD/FMN-containing dehydrogenase -
  QQ016_RS13230 - 2968754..2969179 (-) 426 WP_285962473.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62381.31 Da        Isoelectric Point: 5.3489

>NTDB_id=842878 QQ016_RS13190 WP_285962466.1 2962675..2964378(+) (pilB) [Pseudomonas tohonis strain KRS022]
MNDSPTLSGLARQLVLAELLDEKSAQNAQQQAQRNRLSLVTYVVQNKLVKSRAIAELSAEQFGVAFFDLSAIDKEGQPKD
LVSEKLLRQHRVIPLWRRGNKLFVGVSDPTNHQAVTDIQFSTGLTTEAILVEDDKLGDAIDKFFDSANSGMEDLGDVDLD
GVDIEAVDDDRQESGAGEGADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEVQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSSCKKEVEVPHETLLKEGFP
EEKIGTFKIFGPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAAQMRKDGFNDLRASGLLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=842878 QQ016_RS13190 WP_285962466.1 2962675..2964378(+) (pilB) [Pseudomonas tohonis strain KRS022]
ATGAATGACTCGCCTACCCTCTCAGGTCTTGCACGCCAGTTGGTCCTCGCTGAACTACTGGATGAGAAAAGTGCGCAGAA
CGCTCAACAACAAGCACAGCGGAACAGGCTTTCGCTGGTTACCTATGTAGTACAGAACAAGCTGGTTAAAAGCAGGGCCA
TTGCAGAGCTGTCAGCAGAGCAGTTTGGCGTCGCTTTCTTCGACCTCAGCGCCATAGACAAGGAGGGGCAGCCAAAAGAC
CTGGTTAGTGAAAAGCTGCTCCGCCAGCATCGAGTGATCCCCCTCTGGCGTAGAGGTAACAAGCTATTCGTTGGAGTTTC
GGACCCTACCAATCACCAGGCTGTGACTGATATCCAGTTCAGCACAGGCCTCACTACAGAGGCGATTCTGGTCGAGGATG
ACAAGCTGGGCGATGCCATCGACAAGTTCTTCGATAGCGCCAACTCAGGCATGGAGGACCTCGGTGACGTCGATCTGGAC
GGCGTTGATATAGAGGCGGTCGACGACGACAGACAAGAGAGCGGTGCAGGTGAAGGCGCTGACGACGCTCCAGTCGTACG
CTTCGTCAATAAAATGCTGCTGGACGCCATCAAGGGCGGCTCCTCCGACTTGCACTTCGAGCCCTACGAGCGCTCGTACC
GCGTAAGGCTGCGGACCGATGGCATCCTTCACGAAGTGGCGCGCCCACCGATTCAACTGGCCAGCCGCATTTCCGCGCGC
CTGAAGGTCATGGCAGCCCTGGATATTTCCGAACGTCGCAAGCCACAGGACGGCCGGATCAAGATGAAAATTTCCAAAAC
AAAAGCCATCGACTTCCGCGTCAACACGCTCCCCACTCTCTGGGGCGAAAAGATCGTGATGCGGATTCTTGACCCATCCA
GCGCACAGATGGGCATTGATGCGCTGGGCTATGAAGAAGTACAAAAAGAACTCTATTTGAATGCACTTAAACAACCTCAG
GGCATGATCCTTGTGACTGGCCCTACAGGGTCAGGCAAGACAGTATCGCTCTACACCGGCATCAATATTCTCAACACCGT
CGACATCAACATTTCAACAGCTGAAGACCCGGTGGAAATCAACCTGGAAGGTATCAACCAGGTCAATGTCAATCCACGTC
AGGGTATGGATTTCGCCCAAGCCCTACGGGCTTTCCTACGCCAGGACCCTGACGTGATCATGGTTGGCGAAATCCGCGAC
TTGGAAACTGCCGAGATCGCCATCAAGGCGGCACAGACCGGTCACATGGTGATGTCCACGCTGCACACCAACAGCGCTGC
GGAAACCCTGACTCGCCTACGCAACATGGGCGTTCCGGCATTCAACATCGCCACCTCTGTCAACCTGATCATCGCCCAGC
GCCTCGCTCGGAAGCTCTGCAGCAGCTGCAAGAAGGAGGTCGAGGTTCCACACGAGACGCTTCTGAAGGAGGGGTTTCCG
GAAGAAAAGATCGGTACGTTCAAGATCTTCGGCCCAGTCGGCTGCGAAAACTGTAAGGGCGGCTACAAAGGTAGGGTCGG
GATTTATGAAGTGGTTAAAAACACGCCGGCCCTGCAACGGATTATCATGGAGGAAGGCAACTCCATCGATATCGCCGCGC
AGATGCGCAAAGATGGCTTCAACGATCTGCGCGCCTCGGGCCTGCTGAAAGCCATGCAAGGCGTAACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.772

100

0.591
  pilB Acinetobacter baylyi ADP1

58.053

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

55.908

100

0.559
  pilB Vibrio cholerae strain A1552

50

98.765

0.494
  pilB Vibrio campbellii strain DS40M4

48.246

100

0.485
  pilF Neisseria gonorrhoeae MS11

48.49

99.295

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

50.37

95.238

0.48
  pilF Thermus thermophilus HB27

37.018

100

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

93.298

0.367