Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ALDI51_RS02590 Genome accession   NZ_AP024172
Coordinates   535171..536280 (-) Length   369 a.a.
NCBI ID   WP_269093193.1    Uniprot ID   -
Organism   Alicycliphilus denitrificans strain I51     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 530171..541280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ALDI51_RS02560 (ALDI51_05080) - 530510..531439 (+) 930 WP_201397091.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  ALDI51_RS02565 (ALDI51_05090) rsmI 531512..532435 (-) 924 WP_201397092.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ALDI51_RS02570 (ALDI51_05100) - 532435..532833 (+) 399 WP_201397093.1 YraN family protein -
  ALDI51_RS02575 (ALDI51_05110) - 532893..533495 (+) 603 WP_201397094.1 SIS domain-containing protein -
  ALDI51_RS02580 (ALDI51_05120) - 533492..534160 (+) 669 WP_201397095.1 BON domain-containing protein -
  ALDI51_RS02585 (ALDI51_05130) - 534164..535096 (-) 933 WP_201397096.1 NAD(P)-dependent oxidoreductase -
  ALDI51_RS02590 (ALDI51_05140) pilU 535171..536280 (-) 1110 WP_269093193.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ALDI51_RS02595 (ALDI51_05150) - 536333..536962 (-) 630 WP_094435389.1 cyclic nucleotide-binding domain-containing protein -
  ALDI51_RS02600 (ALDI51_05160) pilT 537026..538069 (-) 1044 WP_094435387.1 type IV pilus twitching motility protein PilT Machinery gene
  ALDI51_RS02605 (ALDI51_05170) - 538109..538831 (+) 723 WP_201397097.1 YggS family pyridoxal phosphate-dependent enzyme -
  ALDI51_RS02610 (ALDI51_05180) - 538872..540002 (+) 1131 WP_201397098.1 aminotransferase class V-fold PLP-dependent enzyme -
  ALDI51_RS02615 (ALDI51_05190) - 540063..540485 (-) 423 WP_094435381.1 DNA-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40570.92 Da        Isoelectric Point: 6.9072

>NTDB_id=83819 ALDI51_RS02590 WP_269093193.1 535171..536280(-) (pilU) [Alicycliphilus denitrificans strain I51]
MNDLLKLMVSRGGSDLFITADFPPAIKVDGKVTKVSPQPLTPVHTLTLARSIMSDKQVADFERTKECNFAIAPAGIGRFR
ANAFIQQGRVGMVLRTIPLTLPTIDGLGVPQVLKEIAMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFGHIITVEDPI
EFVHPHKNCVVTQREVGLDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSANQALDRIVN
FFPEERRAQLLMDLSLNLKAMVSQRLIPKQDGKGRAAAVEVMLNTPLIADLIFKGDVAEIKEIMKKSRNLGMQTFDQSLF
DLFEANVISYEDALRNADSINDLRLQIKLGSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=83819 ALDI51_RS02590 WP_269093193.1 535171..536280(-) (pilU) [Alicycliphilus denitrificans strain I51]
ATCAACGACCTGCTCAAGCTGATGGTCAGCCGGGGCGGCAGCGACCTGTTCATCACGGCCGACTTTCCGCCGGCCATCAA
GGTGGACGGCAAGGTCACCAAGGTCTCGCCCCAGCCGCTCACGCCCGTGCACACGCTGACGCTGGCGCGCTCCATCATGA
GCGACAAGCAGGTGGCCGACTTCGAGCGCACCAAGGAGTGCAATTTCGCCATAGCCCCCGCGGGCATAGGGCGCTTTCGC
GCCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGCTGCGCACCATCCCGCTGACGCTGCCCACCATCGACGGCCT
GGGCGTGCCGCAGGTGCTCAAGGAGATCGCGATGACCAAGCGCGGCCTGTGCATCCTCGTGGGCGCCACGGGCTCGGGCA
AGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAACTCCTTCGGCCACATCATCACCGTCGAGGACCCCATC
GAGTTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAGGTGGGGCTCGATACCGACAGCTGGGAAGCGGCGCT
GAAGAACACCCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAGATCCGCGACCGCGAGACCATGGAGCACGCCGTGG
CCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCCAACCAGGCGCTGGACCGCATCGTCAAC
TTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTCATGGACCTGTCGCTGAACCTCAAGGCCATGGTCTCGCAGCGCCTGAT
CCCCAAGCAGGACGGCAAGGGCCGCGCGGCGGCCGTCGAGGTCATGCTCAACACCCCGCTGATCGCCGACCTGATCTTCA
AGGGCGATGTCGCCGAGATCAAGGAGATCATGAAAAAGAGCCGCAACCTGGGCATGCAGACCTTCGACCAGTCGCTGTTC
GACCTGTTCGAGGCCAACGTCATCAGCTACGAGGACGCCCTGCGCAACGCCGACTCGATCAACGACCTGCGCCTGCAGAT
CAAGCTCGGCAGCCAGCGCGCCAAGACCACCGACCTGGCCTCGGGCACCGAGCATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.47

94.58

0.61
  pilU Acinetobacter baylyi ADP1

57.66

97.29

0.561
  pilU Vibrio cholerae strain A1552

54.023

94.309

0.509
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

91.328

0.415
  pilT Pseudomonas aeruginosa PAK

45.104

91.328

0.412
  pilT Acinetobacter nosocomialis M2

44.745

90.244

0.404
  pilT Acinetobacter baumannii D1279779

44.745

90.244

0.404
  pilT Acinetobacter baumannii strain A118

44.745

90.244

0.404
  pilT Legionella pneumophila strain ERS1305867

44.411

89.702

0.398
  pilT Legionella pneumophila strain Lp02

44.411

89.702

0.398
  pilT Pseudomonas stutzeri DSM 10701

43.62

91.328

0.398
  pilT Acinetobacter baylyi ADP1

42.643

90.244

0.385
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

89.431

0.374
  pilT Vibrio cholerae strain A1552

41.818

89.431

0.374

Multiple sequence alignment