NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	QMK55_RS08920	Genome accession	NZ_CP125378
Coordinates	1985256..1985696 (-)	Length	146 a.a.
NCBI ID	WP_102356464.1	Uniprot ID	A0A2N7RA05
Organism	Pseudomonas sp. P8_229
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1980256..1990696

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
QMK55_RS08905 (QMK55_08900)	-	1981091..1984039 (+)	2949	WP_320329060.1	DUF748 domain-containing protein	-
QMK55_RS08910 (QMK55_08905)	-	1984039..1984335 (+)	297	WP_102356462.1	DUF2845 domain-containing protein	-
QMK55_RS08915 (QMK55_08910)	-	1984607..1984963 (-)	357	WP_320329061.1	BON domain-containing protein	-
QMK55_RS08920 (QMK55_08915)	pilA	1985256..1985696 (-)	441	WP_102356464.1	pilin	Machinery gene
QMK55_RS08925 (QMK55_08920)	pilB	1985922..1987622 (+)	1701	WP_320329062.1	type IV-A pilus assembly ATPase PilB	Machinery gene
QMK55_RS08930 (QMK55_08925)	pilC	1987625..1988842 (+)	1218	WP_102356466.1	type II secretion system F family protein	Machinery gene
QMK55_RS08935 (QMK55_08930)	pilD	1988844..1989716 (+)	873	WP_102356467.1	prepilin peptidase	Machinery gene
QMK55_RS08940 (QMK55_08935)	coaE	1989713..1990336 (+)	624	WP_102356468.1	dephospho-CoA kinase	-
QMK55_RS08945 (QMK55_08940)	yacG	1990333..1990533 (+)	201	WP_102356469.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 146 a.a. Molecular weight: 14574.71 Da Isoelectric Point: 8.1087

                                >NTDB_id=831612 QMK55_RS08920 WP_102356464.1 1985256..1985696(-) (pilA) [Pseudomonas sp. P8_229]

MKQQQGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLLLVAGSATATTSNCT

LAASGTASTGAGTITCTLVNAPGPVLGKTITLTRTGAATGNTSGVWSCASTVNPDYAPKGCAGTGA

Nucleotide

Download Length: 441 bp

                                >NTDB_id=831612 QMK55_RS08920 WP_102356464.1 1985256..1985696(-) (pilA) [Pseudomonas sp. P8_229]

ATGAAACAACAACAGGGCTTTACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATTGGCATTCTGGCGACTATCGCCTT

GCCGCAATACTCCAAATACCAGGCACGTTCGAAAGTGACTGCGGGTCTGGCGGAGATCAGTGCACTGAAGGTGCCGTTCG

AGGACACCATCAATCAAGGCACTGATCCAACGCTATTACTGGTGGCCGGTAGCGCTACAGCGACCACCTCCAACTGCACG

CTGGCGGCGTCGGGAACCGCCTCGACCGGTGCCGGCACTATCACCTGCACACTGGTGAATGCTCCAGGTCCGGTGCTCGG

TAAAACCATTACGTTGACCCGTACCGGTGCTGCAACCGGCAACACCTCCGGCGTCTGGAGCTGTGCGTCGACCGTCAACC

CGGACTACGCGCCTAAGGGTTGCGCAGGCACCGGTGCCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A2N7RA05

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Acinetobacter baumannii strain A118	48.299	100	0.486
pilA	Pseudomonas aeruginosa PAK	42.208	100	0.445
pilA	Vibrio cholerae strain A1552	42.667	100	0.438
pilA	Vibrio cholerae C6706	42.667	100	0.438
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	42.667	100	0.438
pilA/pilAII	Pseudomonas stutzeri DSM 10701	44.681	96.575	0.432
pilA/pilAI	Pseudomonas stutzeri DSM 10701	44.366	97.26	0.432
comP	Acinetobacter baylyi ADP1	39.103	100	0.418
pilA	Ralstonia pseudosolanacearum GMI1000	37.195	100	0.418
pilA	Vibrio parahaemolyticus RIMD 2210633	41.135	96.575	0.397
pilA2	Legionella pneumophila str. Paris	40.141	97.26	0.39
pilA2	Legionella pneumophila strain ERS1305867	40.141	97.26	0.39

Detailed information NTG0831612