Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   QMK55_RS08930 Genome accession   NZ_CP125378
Coordinates   1987625..1988842 (+) Length   405 a.a.
NCBI ID   WP_102356466.1    Uniprot ID   A0A2N7R9Z4
Organism   Pseudomonas sp. P8_229     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1982625..1993842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK55_RS08910 (QMK55_08905) - 1984039..1984335 (+) 297 WP_102356462.1 DUF2845 domain-containing protein -
  QMK55_RS08915 (QMK55_08910) - 1984607..1984963 (-) 357 WP_320329061.1 BON domain-containing protein -
  QMK55_RS08920 (QMK55_08915) pilA 1985256..1985696 (-) 441 WP_102356464.1 pilin Machinery gene
  QMK55_RS08925 (QMK55_08920) pilB 1985922..1987622 (+) 1701 WP_320329062.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK55_RS08930 (QMK55_08925) pilC 1987625..1988842 (+) 1218 WP_102356466.1 type II secretion system F family protein Machinery gene
  QMK55_RS08935 (QMK55_08930) pilD 1988844..1989716 (+) 873 WP_102356467.1 prepilin peptidase Machinery gene
  QMK55_RS08940 (QMK55_08935) coaE 1989713..1990336 (+) 624 WP_102356468.1 dephospho-CoA kinase -
  QMK55_RS08945 (QMK55_08940) yacG 1990333..1990533 (+) 201 WP_102356469.1 DNA gyrase inhibitor YacG -
  QMK55_RS08950 (QMK55_08945) - 1990540..1990755 (-) 216 WP_007909901.1 hypothetical protein -
  QMK55_RS08955 (QMK55_08950) - 1990821..1991510 (-) 690 WP_320329063.1 energy-coupling factor ABC transporter permease -
  QMK55_RS08960 (QMK55_08955) - 1991629..1992255 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -
  QMK55_RS08965 (QMK55_08960) - 1992252..1992779 (+) 528 WP_320329064.1 MOSC domain-containing protein -
  QMK55_RS08970 (QMK55_08965) - 1992831..1993004 (+) 174 WP_102356472.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44126.91 Da        Isoelectric Point: 10.1093

>NTDB_id=831614 QMK55_RS08930 WP_102356466.1 1987625..1988842(+) (pilC) [Pseudomonas sp. P8_229]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSVSLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
EALKAKIKKAMTYPTAVVLVAMVVTGILLVKVVPQFQAVFSGFGAELPAFTLMVISISEFMQQWWWAVLGALIAVFFGTR
HALRKSQALRDRKDTWLLKVPLVGTLMYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVATGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDDMLDKVAGFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=831614 QMK55_RS08930 WP_102356466.1 1987625..1988842(+) (pilC) [Pseudomonas sp. P8_229]
ATGGCGGTCAAGGCAGCGAAAATCAGCATCTACGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAGGTCACCGGCGAACT
GAGCGGGCAGAACCCCGCGTTGATCAAGGCCCAGCTACGTAAACAAGGGATCAACCCCGGCAAGGTGCGCAAGAAATCCG
TCTCCCTGCTGAGTTTCGGCAAACGCATCAAGGCCCAGGACATCGCGTTGTTCACCCGCCAGATGGCGACCATGATGAAA
GCCGGCGTGCCGCTGCTGCAGTCGTTCGATATCATTGGCGAAGGCTTCGACAACCCGGCGATGCGCAAACTGGTGGACGA
GGTGAAGCAGGAGGTTGCCGCCGGCAACAGCTTCGCCACCGCCCTGCGCAAGAAACCGCAGTATTTCGATGAACTGTATT
GCAACCTGGTGGATGCCGGCGAGCAGTCCGGCGCGCTCGACACGTTGCTGGAGCGAGTCGCGACCTATAAAGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCCACCGCCGTGGTGCTGGTGGCAATGGTCGTCACCGGGAT
TTTGCTGGTGAAAGTGGTGCCGCAGTTTCAGGCGGTATTTTCCGGCTTCGGTGCTGAACTGCCGGCCTTCACGCTGATGG
TGATCAGCATTTCCGAATTCATGCAGCAATGGTGGTGGGCGGTGCTTGGTGCACTGATTGCGGTGTTTTTCGGTACGCGT
CACGCCCTGAGAAAGTCCCAGGCACTGCGCGACCGCAAAGACACCTGGCTGCTGAAAGTGCCGTTGGTGGGCACACTGAT
GTACAAGTCGGCGGTGGCCCGATTCGCCCGTACCCTGTCGACCACTTTCGCTGCCGGCGTACCGCTGGTGGAGGCACTGG
ATTCGGTGGCCGGGGCCACCGGCAATGTGGTGTTCAAGCGCGCGGTGCTGCGCATCCGCCAGGACGTCGCCACCGGCATG
CAGCTGAATTTTTCGATGCGCAGCACAGGAGTGTTCCCCAACATGGCGGTGCAGATGACCGCCATCGGCGAGGAATCCGG
CGCGCTGGATGACATGCTCGACAAAGTCGCCGGTTTTTATGAGGACGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TGATGGAGCCGTTCATCATGGTGGTGCTCGGTGTCATCGTCGGCGGTCTGGTCGTGGCCATGTACTTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2N7R9Z4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Acinetobacter baylyi ADP1

58.151

100

0.59
  pilC Legionella pneumophila strain ERS1305867

53.5

98.765

0.528
  pilG Neisseria meningitidis 44/76-A

43.21

100

0.432
  pilG Neisseria gonorrhoeae MS11

43.532

99.259

0.432
  pilC Vibrio cholerae strain A1552

43.939

97.778

0.43
  pilC Vibrio campbellii strain DS40M4

41.379

100

0.415
  pilC Thermus thermophilus HB27

38.404

99.012

0.38