Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QMK55_RS08925 Genome accession   NZ_CP125378
Coordinates   1985922..1987622 (+) Length   566 a.a.
NCBI ID   WP_320329062.1    Uniprot ID   -
Organism   Pseudomonas sp. P8_229     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1980922..1992622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK55_RS08905 (QMK55_08900) - 1981091..1984039 (+) 2949 WP_320329060.1 DUF748 domain-containing protein -
  QMK55_RS08910 (QMK55_08905) - 1984039..1984335 (+) 297 WP_102356462.1 DUF2845 domain-containing protein -
  QMK55_RS08915 (QMK55_08910) - 1984607..1984963 (-) 357 WP_320329061.1 BON domain-containing protein -
  QMK55_RS08920 (QMK55_08915) pilA 1985256..1985696 (-) 441 WP_102356464.1 pilin Machinery gene
  QMK55_RS08925 (QMK55_08920) pilB 1985922..1987622 (+) 1701 WP_320329062.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QMK55_RS08930 (QMK55_08925) pilC 1987625..1988842 (+) 1218 WP_102356466.1 type II secretion system F family protein Machinery gene
  QMK55_RS08935 (QMK55_08930) pilD 1988844..1989716 (+) 873 WP_102356467.1 prepilin peptidase Machinery gene
  QMK55_RS08940 (QMK55_08935) coaE 1989713..1990336 (+) 624 WP_102356468.1 dephospho-CoA kinase -
  QMK55_RS08945 (QMK55_08940) yacG 1990333..1990533 (+) 201 WP_102356469.1 DNA gyrase inhibitor YacG -
  QMK55_RS08950 (QMK55_08945) - 1990540..1990755 (-) 216 WP_007909901.1 hypothetical protein -
  QMK55_RS08955 (QMK55_08950) - 1990821..1991510 (-) 690 WP_320329063.1 energy-coupling factor ABC transporter permease -
  QMK55_RS08960 (QMK55_08955) - 1991629..1992255 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62610.50 Da        Isoelectric Point: 5.8283

>NTDB_id=831613 QMK55_RS08925 WP_320329062.1 1985922..1987622(+) (pilB) [Pseudomonas sp. P8_229]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVRSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLSDAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQENIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFSQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPP
EQISSFTIYEPVGCDQCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDSQMRHDGFDDLRTSGLRKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=831613 QMK55_RS08925 WP_320329062.1 1985922..1987622(+) (pilB) [Pseudomonas sp. P8_229]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAACTGGTACAGGCTGAACTGTTGACGGAAAAAAGCGCCCAGCAAGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAGGAGTTCCCAGGTCG
CCGAAATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGTCAGCACCACGCCCTGCCCTTGTGGCGACGCGGCAACAAGCTGTTCGTGGGCATTTCCGA
TCCGAGCAATCATCAGGCAATCAACGATATCCAGTTCAGCACCGGACTGAGTACCGAAGCCATCCTGGTGGAAGACGACA
AACTCAGCGATGCCATCGAGAAATTTTTCGACACCCACGCCAGCGGCCTCGAAGAGATGGCCGACGTCGACCTCGACGGG
GTGGACGTCGAATCCGTCGACGACAGCCGACAGGAGAATATCGGCGGACTCGATGCCGATGACGCCCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGTGGCTCGTCGGACTTGCACTTCGAACCCTACGAAAAGAACTTCCGGG
TGCGGGTGCGCACCGACGGCATCCTGCGCGAAGTGGCCAAACCGCCGATTCAGTTGGCCGGGCGGATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGACATCTCGGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGAGCAA
GTCGATCGACTTCCGGGTCAACACCCTGCCCACCCTGTGGGGCGAGAAAGTGGTGATCCGGATTCTCGATCCGTCCAGCG
CGCAGATCGGCATCGATGCCCTGGGTTACGAACCGGCGCAGAAAGACCTGTACATGGCCGCGCTCAAGCAGCCGCAGGGG
ATGATTCTGGTGACCGGCCCGACCGGGTCGGGGAAAACCGTGTCGCTGTACACCGGACTCAATATTCTCAATACCGTCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCGAAACAGG
GGCTGGATTTCTCCCAGGCATTACGCTCGTTTCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAAATTCGCGATCTG
GAAACCGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACCTCGTGTTGTCCACGCTGCACACCAACAGCGCCGCCGA
AACCCTGACCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGCC
TGGCACGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATTCCGCGCGAAACGCTGATCAAGGAAGGCTTCCCACCG
GAACAGATCAGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGATCAATGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCCGAGCTGCAACGGCTGATCATGGCCGAAGGCAACTCGCTGGAAATCGACAGCCAGA
TGCGTCACGACGGTTTCGACGATCTGCGCACCTCAGGCCTGCGCAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.876

99.823

0.578
  pilB Acinetobacter baumannii D1279779

57.624

99.647

0.574
  pilB Legionella pneumophila strain ERS1305867

55.752

99.823

0.557
  pilB Vibrio cholerae strain A1552

50.973

99.823

0.509
  pilB Vibrio campbellii strain DS40M4

50

99.647

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

49.911

99.117

0.495
  pilF Neisseria gonorrhoeae MS11

49.378

99.47

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.752

93.463

0.362
  pilF Thermus thermophilus HB27

36.738

98.587

0.362