Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   R3J28_RS10835 Genome accession   NZ_CP136971
Coordinates   2328968..2329408 (+) Length   146 a.a.
NCBI ID   WP_375732100.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain Santa29b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2323968..2334408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J28_RS10815 (R3J28_10805) coaE 2324066..2324689 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  R3J28_RS10820 (R3J28_10810) pilD 2324701..2325564 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  R3J28_RS10825 (R3J28_10815) pilC 2325571..2326773 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  R3J28_RS10830 (R3J28_10820) pilA/pilAI 2327214..2327660 (+) 447 WP_154128445.1 pilin Machinery gene
  R3J28_RS10835 (R3J28_10825) pilA/pilAI 2328968..2329408 (+) 441 WP_375732100.1 pilin Machinery gene
  R3J28_RS10840 (R3J28_10830) pilB 2330248..2331981 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene
  R3J28_RS10845 (R3J28_10835) - 2332075..2333487 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14967.21 Da        Isoelectric Point: 8.1156

>NTDB_id=816907 R3J28_RS10835 WP_375732100.1 2328968..2329408(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain Santa29b]
MKKQQGFTLIELMIVIAIIAILAAIALPMYQNYVARSQVTAGLAEITPSKAQAEIRMADGQASNTPNAIGLRTPTARCSL
VTVDITTTGGTIVCTMVGNGQVNNQTITLTRIADNNAGQGGVNTGGNWTCTTTAPAALTPAGCTGT

Nucleotide


Download         Length: 441 bp        

>NTDB_id=816907 R3J28_RS10835 WP_375732100.1 2328968..2329408(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain Santa29b]
ATGAAAAAGCAACAAGGCTTCACTTTGATCGAGCTGATGATCGTCATTGCGATCATTGCCATCCTGGCAGCGATCGCTCT
GCCCATGTACCAAAATTATGTCGCCAGGTCTCAAGTCACAGCGGGACTGGCCGAAATTACACCCAGCAAAGCACAAGCCG
AAATCCGTATGGCTGATGGACAGGCTTCAAACACTCCTAACGCTATTGGATTACGCACCCCGACCGCTCGTTGCAGCCTC
GTTACGGTCGACATCACAACTACTGGCGGCACAATCGTATGTACGATGGTTGGTAATGGTCAGGTCAACAATCAAACTAT
CACCTTGACCCGCATCGCTGATAACAACGCAGGCCAAGGTGGCGTCAATACTGGGGGGAACTGGACCTGTACCACAACCG
CGCCTGCAGCGTTAACTCCAGCAGGCTGCACTGGAACCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

44.218

100

0.445
  pilA Vibrio cholerae C6706

39.13

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

39.13

100

0.432
  pilA Vibrio cholerae strain A1552

39.13

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.466

100

0.425
  pilA Acinetobacter baumannii strain A118

40.541

100

0.411
  pilA Pseudomonas aeruginosa PAK

37.107

100

0.404
  pilA Haemophilus influenzae 86-028NP

38.514

100

0.39
  pilA Vibrio parahaemolyticus RIMD 2210633

39.31

99.315

0.39
  comP Acinetobacter baylyi ADP1

36.538

100

0.39
  pilA Haemophilus influenzae Rd KW20

37.838

100

0.384
  pilA2 Legionella pneumophila strain ERS1305867

39.161

97.945

0.384
  pilA2 Legionella pneumophila str. Paris

39.161

97.945

0.384