Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   R3J28_RS10830 Genome accession   NZ_CP136971
Coordinates   2327214..2327660 (+) Length   148 a.a.
NCBI ID   WP_154128445.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. multiplex strain Santa29b     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2322214..2332660
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R3J28_RS10810 (R3J28_10800) - 2322462..2323799 (+) 1338 WP_027700147.1 HAMP domain-containing sensor histidine kinase -
  R3J28_RS10815 (R3J28_10805) coaE 2324066..2324689 (-) 624 WP_004084593.1 dephospho-CoA kinase -
  R3J28_RS10820 (R3J28_10810) pilD 2324701..2325564 (-) 864 WP_004084594.1 A24 family peptidase Machinery gene
  R3J28_RS10825 (R3J28_10815) pilC 2325571..2326773 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  R3J28_RS10830 (R3J28_10820) pilA/pilAI 2327214..2327660 (+) 447 WP_154128445.1 pilin Machinery gene
  R3J28_RS10835 (R3J28_10825) pilA/pilAI 2328968..2329408 (+) 441 WP_375732100.1 pilin Machinery gene
  R3J28_RS10840 (R3J28_10830) pilB 2330248..2331981 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15389.59 Da        Isoelectric Point: 8.4988

>NTDB_id=816906 R3J28_RS10830 WP_154128445.1 2327214..2327660(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain Santa29b]
MKKQQGFNLIELMIVIAIIAVLAAIALPMYQNYVARSQLTAALADITPGKVQAESLIADGKGTSNASDIGLRTDTTRCGI
TVKVDTAGTANITCKIKGNSQVNDKTIAWDRTPDNSAGTNGVNNGGVWTCSSTVTSDALRPSGCMAAK

Nucleotide


Download         Length: 447 bp        

>NTDB_id=816906 R3J28_RS10830 WP_154128445.1 2327214..2327660(+) (pilA/pilAI) [Xylella fastidiosa subsp. multiplex strain Santa29b]
ATGAAAAAGCAACAAGGTTTTAACTTAATCGAACTAATGATCGTCATTGCAATCATTGCTGTTCTGGCGGCCATCGCTCT
GCCCATGTACCAAAATTATGTTGCCAGATCCCAACTCACTGCGGCACTTGCCGACATCACGCCGGGCAAAGTGCAAGCCG
AGTCTCTGATTGCTGATGGGAAGGGTACATCCAACGCGAGTGATATCGGTCTGCGTACCGACACCACTCGTTGTGGAATT
ACCGTTAAAGTCGATACCGCTGGCACTGCCAACATCACATGCAAAATTAAGGGCAACTCTCAAGTCAATGATAAAACCAT
CGCTTGGGATCGTACCCCCGATAACTCTGCAGGCACCAATGGCGTCAATAATGGAGGTGTGTGGACCTGCTCCTCCACCG
TCACCTCCGATGCACTGAGACCTTCCGGCTGCATGGCTGCTAAATAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.537

99.324

0.432
  pilA Pseudomonas aeruginosa PAK

38.994

100

0.419
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.177

99.324

0.419
  pilA Vibrio cholerae strain A1552

38.71

100

0.405
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.71

100

0.405
  pilA Vibrio cholerae C6706

38.71

100

0.405
  pilA Acinetobacter baumannii strain A118

39.333

100

0.399