Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   Thiofri_RS19710 Genome accession   NZ_CP121471
Coordinates   4315421..4315852 (+) Length   143 a.a.
NCBI ID   WP_009147853.1    Uniprot ID   H8Z0G6
Organism   Thiorhodovibrio frisius strain 970     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4310421..4320852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Thiofri_RS19650 (Thiofri_04039) - 4310426..4310686 (-) 261 WP_009147843.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
  Thiofri_RS19655 (Thiofri_04040) - 4310929..4311147 (+) 219 WP_009147844.1 antitoxin MazE family protein -
  Thiofri_RS19660 (Thiofri_04041) - 4311148..4311471 (+) 324 WP_009147845.1 type II toxin-antitoxin system PemK/MazF family toxin -
  Thiofri_RS19665 (Thiofri_04042) - 4311556..4311810 (+) 255 WP_051023812.1 hypothetical protein -
  Thiofri_RS19670 - 4311807..4311935 (+) 129 WP_255324711.1 hypothetical protein -
  Thiofri_RS19675 (Thiofri_04043) - 4311957..4312226 (-) 270 WP_009147846.1 SEL1-like repeat protein -
  Thiofri_RS19680 (Thiofri_04044) - 4312272..4312820 (-) 549 WP_009147847.1 hypothetical protein -
  Thiofri_RS19685 (Thiofri_04045) - 4312834..4313079 (-) 246 WP_255324712.1 DUF1778 domain-containing protein -
  Thiofri_RS19690 (Thiofri_04046) - 4313193..4313645 (-) 453 WP_009147849.1 hypothetical protein -
  Thiofri_RS19695 (Thiofri_04047) - 4313675..4314022 (-) 348 WP_009147850.1 type II toxin-antitoxin system PemK/MazF family toxin -
  Thiofri_RS19700 (Thiofri_04048) - 4314022..4314267 (-) 246 WP_009147851.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  Thiofri_RS19705 (Thiofri_04049) - 4314370..4314831 (-) 462 WP_009147852.1 SEL1-like repeat protein -
  Thiofri_RS19710 (Thiofri_04050) pilE 4315421..4315852 (+) 432 WP_009147853.1 pilin Machinery gene
  Thiofri_RS19715 (Thiofri_04051) - 4316016..4317935 (+) 1920 WP_009147854.1 hypothetical protein -
  Thiofri_RS19720 (Thiofri_04052) - 4318347..4318544 (+) 198 WP_009147855.1 hypothetical protein -
  Thiofri_RS19725 - 4318566..4319107 (+) 542 Protein_3918 DUF2887 domain-containing protein -
  Thiofri_RS19730 - 4319162..4319419 (+) 258 WP_313778271.1 DUF4351 domain-containing protein -
  Thiofri_RS19735 - 4319738..4320037 (+) 300 WP_083848437.1 hypothetical protein -
  Thiofri_RS19740 - 4320024..4320209 (+) 186 WP_143741849.1 hypothetical protein -
  Thiofri_RS19745 (Thiofri_04054) - 4320251..4320835 (+) 585 WP_009147857.1 Uma2 family endonuclease -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 14725.91 Da        Isoelectric Point: 6.2231

>NTDB_id=812305 Thiofri_RS19710 WP_009147853.1 4315421..4315852(+) (pilE) [Thiorhodovibrio frisius strain 970]
MRKQIQKGFTLIELMIVVAIIGILAAIAIPAYQDYVARAQMSEAFSLASGSKTAVVEFWSREGGWPTGNASAGLATNTAI
SGKYVAQVDVSGGPIVATMQATGVSAGIQGETLTLSPVTTAGAVEWVCTSSADAKYIPGNCRP

Nucleotide


Download         Length: 432 bp        

>NTDB_id=812305 Thiofri_RS19710 WP_009147853.1 4315421..4315852(+) (pilE) [Thiorhodovibrio frisius strain 970]
ATGCGTAAGCAAATTCAGAAAGGTTTTACCCTGATCGAACTCATGATCGTTGTGGCGATCATTGGTATTCTGGCGGCGAT
TGCGATTCCTGCGTATCAGGATTATGTTGCGCGTGCCCAAATGTCTGAGGCATTCAGCCTTGCCAGTGGGTCAAAAACAG
CCGTTGTTGAGTTTTGGAGTCGTGAAGGTGGTTGGCCAACTGGCAATGCATCTGCTGGTTTGGCCACGAACACTGCTATT
TCTGGTAAATATGTGGCGCAAGTTGACGTATCCGGTGGTCCAATTGTAGCGACAATGCAGGCCACGGGCGTTTCAGCAGG
TATCCAAGGCGAAACCTTAACTCTTAGTCCGGTCACAACCGCTGGCGCGGTAGAGTGGGTTTGTACGTCTAGCGCAGATG
CTAAGTACATACCCGGCAATTGTCGTCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-28)

Pilin (bacterial filament) (Pilin)

(37-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB H8Z0G6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae MS11

48.077

100

0.524
  pilE Neisseria gonorrhoeae strain FA1090

47.742

100

0.517
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.508

100

0.483
  pilA2 Legionella pneumophila str. Paris

47.518

98.601

0.469
  comP Acinetobacter baylyi ADP1

45.578

100

0.469
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.624

100

0.455
  pilA Ralstonia pseudosolanacearum GMI1000

38.235

100

0.455
  pilA2 Legionella pneumophila strain ERS1305867

44.286

97.902

0.434
  pilA Pseudomonas aeruginosa PAK

42.177

100

0.434
  pilA/pilA1 Eikenella corrodens VA1

39.355

100

0.427
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40.69

100

0.413
  pilA Acinetobacter baumannii strain A118

41.429

97.902

0.406
  pilA Haemophilus influenzae 86-028NP

38.462

100

0.385
  pilA Haemophilus influenzae Rd KW20

39.286

97.902

0.385