Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PY254_RS17335 Genome accession   NZ_CP119392
Coordinates   3892669..3893781 (+) Length   370 a.a.
NCBI ID   WP_281013303.1    Uniprot ID   -
Organism   Rhodanobacter sp. AS-Z3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3887669..3898781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY254_RS17315 (PY254_17315) - 3889220..3890590 (-) 1371 WP_281013299.1 pyridoxal-phosphate dependent enzyme -
  PY254_RS17320 (PY254_17320) - 3890804..3891031 (-) 228 WP_281013300.1 YdcH family protein -
  PY254_RS17325 (PY254_17325) - 3891371..3892012 (-) 642 WP_281013301.1 hypothetical protein -
  PY254_RS17330 (PY254_17330) - 3892009..3892467 (-) 459 WP_281013302.1 DUF4398 domain-containing protein -
  PY254_RS17335 (PY254_17335) pilU 3892669..3893781 (+) 1113 WP_281013303.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PY254_RS17340 (PY254_17340) maiA 3893852..3894520 (-) 669 WP_281013304.1 maleylacetoacetate isomerase -
  PY254_RS17345 (PY254_17345) - 3894691..3895875 (+) 1185 WP_281013305.1 phosphoglycerate kinase -
  PY254_RS17350 (PY254_17350) - 3895875..3896516 (+) 642 WP_281013306.1 HAD hydrolase-like protein -
  PY254_RS17355 (PY254_17355) tkt 3896694..3898703 (-) 2010 WP_281013307.1 transketolase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 41211.42 Da        Isoelectric Point: 6.5100

>NTDB_id=798801 PY254_RS17335 WP_281013303.1 3892669..3893781(+) (pilU) [Rhodanobacter sp. AS-Z3]
MDIGYFLKLMVDKGASDMFLSTGAPVNIKVEGKLYPLGNTGLPTGMVKKIAYSLMDEGQVPQFERNLELNMALAVKEAGR
FRINVFKQRGEVGMVIRAIKSEIPSIEQLQLPKIFRDLIMEPRGLILVVGATGSGKSTTLAAMLDHRNTNSSGHILTIED
PIEYLHRHKKSIVNQREVGLDTHTYHEALKNAMREAPDVIMIGEIRDTDTMEAAIAFSETGHLCLATLHSNNADQTLERI
LNFFPESAHKNVLMNLALNLRAVISQRLVIGKDGRRIPAAEVLLNTPLIRDMIRRGQTHEIKEAMDRSLQEGMQTFDQSL
YRLYKDGRVDLEEALNKSDSRDGLALKIRLAEGGSNDEELAGNDPYGMGF

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=798801 PY254_RS17335 WP_281013303.1 3892669..3893781(+) (pilU) [Rhodanobacter sp. AS-Z3]
GTGGATATCGGTTACTTCCTCAAGCTTATGGTAGACAAGGGCGCGTCGGACATGTTCCTGTCCACCGGCGCTCCGGTAAA
TATCAAGGTGGAGGGCAAACTGTATCCCCTGGGCAATACCGGCCTTCCCACCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCCCAGTTCGAGCGCAATCTGGAACTGAACATGGCGCTGGCGGTGAAGGAAGCCGGCCGT
TTCCGCATCAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATTCGCGCGATCAAGAGCGAAATCCCCAGCATCGA
GCAATTGCAGCTGCCGAAGATTTTCCGTGACCTGATCATGGAGCCACGCGGGCTGATTCTGGTGGTTGGCGCCACCGGCT
CGGGCAAGTCGACCACATTGGCAGCGATGCTCGACCATCGCAACACCAATTCTTCCGGTCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGAAGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGATACGCACACCTATCACGA
GGCGCTGAAGAACGCGATGCGTGAGGCGCCGGACGTCATCATGATCGGCGAGATCCGTGACACCGACACGATGGAAGCGG
CGATCGCGTTCTCCGAAACCGGTCATTTGTGCCTGGCCACGCTGCATTCGAACAACGCCGACCAGACGCTGGAACGCATC
CTCAATTTCTTCCCCGAATCGGCACACAAGAACGTGCTGATGAATCTGGCGCTGAACCTTCGCGCGGTAATCAGCCAGCG
CCTGGTGATCGGCAAGGACGGCCGCCGCATTCCCGCTGCCGAAGTGCTGCTGAACACGCCACTGATCCGCGACATGATCC
GTCGTGGCCAGACTCACGAGATCAAGGAAGCGATGGATCGCAGCCTGCAGGAAGGCATGCAGACGTTCGACCAGTCGTTG
TACCGGCTGTACAAGGACGGACGCGTGGACTTGGAGGAAGCGCTCAACAAGTCCGATTCGCGCGACGGTCTGGCACTCAA
AATCCGCCTCGCCGAAGGCGGCTCCAACGACGAGGAACTGGCCGGCAACGATCCTTACGGCATGGGCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.111

97.297

0.497
  pilU Vibrio cholerae strain A1552

47.838

100

0.478
  pilU Acinetobacter baylyi ADP1

48.87

95.676

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.543

91.622

0.408
  pilT Acinetobacter baylyi ADP1

42.814

90.27

0.386
  pilT Acinetobacter nosocomialis M2

42.216

90.27

0.381
  pilT Acinetobacter baumannii D1279779

42.216

90.27

0.381
  pilT Acinetobacter baumannii strain A118

42.216

90.27

0.381
  pilT Legionella pneumophila strain Lp02

40.936

92.432

0.378
  pilT Legionella pneumophila strain ERS1305867

40.936

92.432

0.378
  pilT Pseudomonas aeruginosa PAK

41.42

91.351

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.124

91.351

0.376
  pilT Vibrio cholerae strain A1552

40.653

91.081

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.653

91.081

0.37