Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PY250_RS12385 Genome accession   NZ_CP119334
Coordinates   2664337..2664786 (+) Length   149 a.a.
NCBI ID   WP_054574816.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659337..2669786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS12370 (PY250_12370) pdhR 2661371..2662138 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PY250_RS12375 (PY250_12375) ampD 2662544..2663095 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PY250_RS12380 (PY250_12380) nadC 2663188..2664075 (+) 888 WP_005386616.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PY250_RS12385 (PY250_12385) pilA 2664337..2664786 (+) 450 WP_054574816.1 pilin Machinery gene
  PY250_RS12390 (PY250_12390) pilB 2664790..2666475 (+) 1686 WP_136976641.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PY250_RS12395 (PY250_12395) pilC 2666499..2667722 (+) 1224 WP_005385411.1 type II secretion system F family protein Machinery gene
  PY250_RS12400 (PY250_12400) pilD 2667787..2668656 (+) 870 WP_136976640.1 prepilin peptidase Machinery gene
  PY250_RS12405 (PY250_12405) coaE 2668657..2669271 (+) 615 WP_213904896.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15569.68 Da        Isoelectric Point: 5.7922

>NTDB_id=798547 PY250_RS12385 WP_054574816.1 2664337..2664786(+) (pilA) [Vibrio alginolyticus strain 2-1]
MKNSKHKEQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGAGLATITSVRTNVEDYIVTNGSFPDGTTAGQLTTD
LGVIQPNNGAITFDDANSNILLTFAGSGNSPDVNNAKIALNRAAGGTWTCKATLDAKLLPKSCSKDANL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=798547 PY250_RS12385 WP_054574816.1 2664337..2664786(+) (pilA) [Vibrio alginolyticus strain 2-1]
ATGAAAAACAGTAAACATAAAGAACAACAGGGTTTTACTCTGATTGAATTAATGATTGTGGTGGCCGTTATTGGTGTTTT
AGCAGCGATTGCTATCCCTCAATACCAAAACTATGTGGCCAAGTCAGAGTTGGGGGCTGGACTAGCAACAATAACCTCAG
TACGAACTAATGTTGAAGATTACATTGTTACAAATGGTAGTTTTCCTGATGGTACAACTGCTGGCCAGCTAACAACTGAC
TTAGGAGTGATTCAGCCAAACAATGGTGCAATAACTTTTGATGACGCCAATAGCAACATCCTGTTAACTTTTGCCGGTTC
TGGAAATAGCCCTGACGTAAATAATGCAAAAATAGCACTAAACAGAGCGGCTGGTGGTACATGGACATGTAAAGCCACCC
TAGATGCTAAACTACTTCCAAAATCATGTTCTAAGGATGCTAATTTATAG

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-143)

Prokaryotic N-terminal methylation motif (N_methyl)

(8-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

52.288

100

0.537
  pilA Vibrio cholerae strain A1552

52.288

100

0.537
  pilA Vibrio cholerae C6706

52.288

100

0.537
  pilA Pseudomonas aeruginosa PAK

45.638

100

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

45.588

91.275

0.416
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.667

96.644

0.403
  pilA Acinetobacter baumannii strain A118

40.58

92.617

0.376
  pilA2 Legionella pneumophila str. Paris

39.855

92.617

0.369
  pilA Acinetobacter nosocomialis M2

37.762

95.973

0.362
  pilA/pilA1 Eikenella corrodens VA1

33.333

100

0.362
  pilA2 Legionella pneumophila strain ERS1305867

39.13

92.617

0.362