Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PY250_RS12395 Genome accession   NZ_CP119334
Coordinates   2666499..2667722 (+) Length   407 a.a.
NCBI ID   WP_005385411.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2661499..2672722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS12375 (PY250_12375) ampD 2662544..2663095 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PY250_RS12380 (PY250_12380) nadC 2663188..2664075 (+) 888 WP_005386616.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PY250_RS12385 (PY250_12385) pilA 2664337..2664786 (+) 450 WP_054574816.1 pilin Machinery gene
  PY250_RS12390 (PY250_12390) pilB 2664790..2666475 (+) 1686 WP_136976641.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PY250_RS12395 (PY250_12395) pilC 2666499..2667722 (+) 1224 WP_005385411.1 type II secretion system F family protein Machinery gene
  PY250_RS12400 (PY250_12400) pilD 2667787..2668656 (+) 870 WP_136976640.1 prepilin peptidase Machinery gene
  PY250_RS12405 (PY250_12405) coaE 2668657..2669271 (+) 615 WP_213904896.1 dephospho-CoA kinase -
  PY250_RS12410 (PY250_12410) zapD 2669299..2670039 (+) 741 WP_136976639.1 cell division protein ZapD -
  PY250_RS12415 (PY250_12415) yacG 2670198..2670392 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  PY250_RS12420 (PY250_12420) rplS 2671047..2671400 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  PY250_RS12425 (PY250_12425) trmD 2671442..2672203 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45359.58 Da        Isoelectric Point: 10.3992

>NTDB_id=798549 PY250_RS12395 WP_005385411.1 2666499..2667722(+) (pilC) [Vibrio alginolyticus strain 2-1]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSVWTQNWSPFIALGSMSLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=798549 PY250_RS12395 WP_005385411.1 2666499..2667722(+) (pilC) [Vibrio alginolyticus strain 2-1]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACGGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTGAAAGGCAGAGACATCACGGTGTTTACCCGTCAGATTTCGACGATGTTG
ATAACCGGTGTACCCTTAGTTCAAGCTTTAAAATTGGTCTCAGAAAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCAGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCTATGGTAGTGCTAGTCGCGTTAGGTGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTTGAAAAAATGTTTGTTGGTTTTGGTGCTGATTTACCTTGGTTTACCAGGC
AAGTATTAGATCTTTCCGTCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATGAGTTTATTCATCTCGGCG
AGAATCCTCTCTAAACGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACATTATCAGCTCGCCATAGAAGAAGTGTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTAGCCATGCGCAACTGTAATGTGTTCCCTGAATTGGTGTTACAAATGGTTATGATTGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACAATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.978

100

0.87
  pilC Vibrio cholerae strain A1552

73.775

100

0.74
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.191

99.017

0.408
  pilC Legionella pneumophila strain ERS1305867

40.295

100

0.403
  pilG Neisseria gonorrhoeae MS11

40.247

99.509

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilG Neisseria meningitidis 44/76-A

39.901

99.754

0.398