Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PY250_RS12390 Genome accession   NZ_CP119334
Coordinates   2664790..2666475 (+) Length   561 a.a.
NCBI ID   WP_136976641.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain 2-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2659790..2671475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY250_RS12370 (PY250_12370) pdhR 2661371..2662138 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PY250_RS12375 (PY250_12375) ampD 2662544..2663095 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PY250_RS12380 (PY250_12380) nadC 2663188..2664075 (+) 888 WP_005386616.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PY250_RS12385 (PY250_12385) pilA 2664337..2664786 (+) 450 WP_054574816.1 pilin Machinery gene
  PY250_RS12390 (PY250_12390) pilB 2664790..2666475 (+) 1686 WP_136976641.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PY250_RS12395 (PY250_12395) pilC 2666499..2667722 (+) 1224 WP_005385411.1 type II secretion system F family protein Machinery gene
  PY250_RS12400 (PY250_12400) pilD 2667787..2668656 (+) 870 WP_136976640.1 prepilin peptidase Machinery gene
  PY250_RS12405 (PY250_12405) coaE 2668657..2669271 (+) 615 WP_213904896.1 dephospho-CoA kinase -
  PY250_RS12410 (PY250_12410) zapD 2669299..2670039 (+) 741 WP_136976639.1 cell division protein ZapD -
  PY250_RS12415 (PY250_12415) yacG 2670198..2670392 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  PY250_RS12420 (PY250_12420) rplS 2671047..2671400 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62096.83 Da        Isoelectric Point: 5.4010

>NTDB_id=798548 PY250_RS12390 WP_136976641.1 2664790..2666475(+) (pilB) [Vibrio alginolyticus strain 2-1]
MLTNLSTALRQKGLLTFSQEESLIEQVKASGISMPEALLSSGFFTSSELAEHLSSLFGLNQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTSSTLLLAVADPTNQQAEDDFRFATGLQVELVLGDFSQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=798548 PY250_RS12390 WP_136976641.1 2664790..2666475(+) (pilB) [Vibrio alginolyticus strain 2-1]
ATGTTAACCAACCTATCCACGGCTCTTCGCCAAAAAGGGTTACTGACGTTTTCCCAAGAAGAATCGTTAATAGAGCAAGT
CAAAGCTTCTGGCATTTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACGTCAAGCGAACTCGCTGAGCACTTAA
GTTCTCTCTTTGGTTTAAATCAGCCCGAGTTATCTCAGTACGAGTATGCTTCGCTTTGTCAGCAACTTGGCCTTAGGGAA
TTAATCACACGGCATAACGCACTCCCACTCCAGCGTACGTCTTCAACGTTATTACTCGCCGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACTGGTTTACAGGTTGAATTGGTCTTGGGGGATTTTAGTCAACTCAGCGCGG
CAATTCGACGCTTGTATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTATTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAGCCTTATGAAAAGATGTACCGCGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTGCCTACTCTATTTGGAGAGAAAATTGTACTACGACTGCTCGACAGCAGCTCTGCGTCAT
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACAGGGAGCGGTAAAACCGTTTCTCTATACACTGGGCTAAGTATCCTCAATAAGCCAGAAATCAA
TATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAGGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCGGATGTGGTAATGGTCGGTGAGATTCGTGACTTAGACACC
GCCGAAATCGCTATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCTGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTGGCCC
GTAAACTGTGCTCGCACTGTAAACAATCACAAGAGCTTACCGTTCAGCTACAACACCTTGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGTAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

95.722

100

0.957
  pilB Vibrio campbellii strain DS40M4

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

74.199

100

0.743
  pilB Acinetobacter baumannii D1279779

51.224

94.652

0.485
  pilB Legionella pneumophila strain ERS1305867

49.353

96.435

0.476
  pilB Acinetobacter baylyi ADP1

50.29

92.157

0.463
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

37.394

100

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.508

100

0.369