Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PW274_RS02425 Genome accession   NZ_CP118775
Coordinates   518996..520132 (-) Length   378 a.a.
NCBI ID   WP_016450725.1    Uniprot ID   A0AAX3SP23
Organism   Delftia tsuruhatensis strain ULwDis3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 513996..525132
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PW274_RS02400 (PW274_02400) rsmI 515017..515958 (-) 942 WP_012202992.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  PW274_RS02405 (PW274_02405) - 515958..516374 (+) 417 WP_016450729.1 YraN family protein -
  PW274_RS02410 (PW274_02410) - 516448..517056 (+) 609 WP_016451992.1 SIS domain-containing protein -
  PW274_RS02415 (PW274_02415) - 517053..517784 (+) 732 WP_016451991.1 BON domain-containing protein -
  PW274_RS02420 (PW274_02420) - 517984..518895 (-) 912 WP_126311196.1 NAD(P)-dependent oxidoreductase -
  PW274_RS02425 (PW274_02425) pilU 518996..520132 (-) 1137 WP_016450725.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PW274_RS02430 (PW274_02430) - 520250..520882 (-) 633 WP_016450724.1 cyclic nucleotide-binding domain-containing protein -
  PW274_RS02435 (PW274_02435) pilT 520914..521957 (-) 1044 WP_016450723.1 type IV pilus twitching motility protein PilT Machinery gene
  PW274_RS02440 (PW274_02440) - 521995..522720 (+) 726 WP_016450722.1 YggS family pyridoxal phosphate-dependent enzyme -
  PW274_RS02445 (PW274_02445) - 522774..524057 (-) 1284 WP_016446679.1 branched-chain amino acid ABC transporter substrate-binding protein -
  PW274_RS02450 (PW274_02450) - 524084..524806 (-) 723 WP_016446678.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42077.62 Da        Isoelectric Point: 6.9079

>NTDB_id=795132 PW274_RS02425 WP_016450725.1 518996..520132(-) (pilU) [Delftia tsuruhatensis strain ULwDis3]
MERDQATKFINDLLKLMVSRNGSDLFITAEFPPAIKIDGRITKVSPQPLSPVHTLTLARSVMSDKQIADFERTKECNFAI
SPPGIGRFRVNAFIQQGRVGMVMRTIPLALPTIDSLGVPQVLKEVTMTKRGLCILVGATGSGKSTTLAAMVDWRNENSYG
HIVTIEDPIEFVHPHKNCVVTQREVGLDTDSWEAALKNSLRQAPDVILMGEIRDRETMEHAIAFSETGHLCLATLHANSA
NQALDRIVNFFPEERRPQLLMDLSLNLRAMVSQRLLPKQDSKGRVAAIEVMLNSPLISDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDLFESNLITYEDALRNADSVNDLRLQIKLGSQRAKSLDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=795132 PW274_RS02425 WP_016450725.1 518996..520132(-) (pilU) [Delftia tsuruhatensis strain ULwDis3]
ATGGAAAGAGATCAGGCCACCAAGTTCATCAACGACCTGCTCAAGCTCATGGTCAGCCGCAACGGCAGCGACTTGTTCAT
CACGGCGGAATTCCCGCCGGCGATCAAGATCGACGGGCGCATCACCAAGGTGTCGCCGCAGCCGCTGTCGCCGGTGCACA
CGCTGACGCTGGCGCGCTCCGTCATGAGCGACAAGCAGATCGCCGATTTCGAGCGCACCAAGGAGTGCAACTTCGCCATC
TCGCCGCCGGGCATAGGGCGCTTTCGCGTCAACGCCTTCATCCAGCAGGGCCGCGTGGGCATGGTGATGCGGACGATTCC
GCTGGCCCTGCCCACCATCGACAGCCTGGGCGTACCCCAGGTGCTCAAGGAAGTGACCATGACCAAGCGCGGCCTGTGCA
TCCTGGTCGGCGCCACGGGCTCGGGCAAGTCCACCACGCTGGCCGCCATGGTGGACTGGCGCAACGAGAATTCCTACGGC
CACATCGTGACCATCGAGGACCCCATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACGCAGCGCGAGGTGGGCCT
GGACACGGACAGCTGGGAAGCAGCGCTCAAGAACTCCCTGCGCCAGGCACCGGACGTGATCCTCATGGGCGAAATCCGGG
ACCGCGAGACCATGGAGCACGCCATCGCCTTCTCGGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGCATCGTCAACTTCTTCCCCGAAGAGCGCCGCCCGCAACTGCTGATGGACCTGTCGCTGAACCT
GCGCGCCATGGTCTCCCAGCGCCTGCTGCCCAAGCAGGACAGCAAGGGACGCGTGGCCGCCATCGAGGTGATGCTCAACT
CGCCGCTGATCTCCGACCTGATCTTCAAGGGCGAAGTCACCGAGATCAAGGAGATCATGAAGAAAAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGTCCAACCTCATCACCTACGAGGATGCCCTGCGCAATGCGGA
CTCGGTCAACGACCTGCGGCTGCAGATCAAGCTGGGCAGCCAGCGCGCCAAGTCGCTGGACCTGGCGGCGGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

56.825

94.974

0.54
  pilU Vibrio cholerae strain A1552

53.736

92.063

0.495
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.697

89.153

0.407
  pilT Pseudomonas aeruginosa PAK

44.214

89.153

0.394
  pilT Pseudomonas stutzeri DSM 10701

43.323

89.153

0.386
  pilT Acinetobacter baumannii D1279779

42.943

88.095

0.378
  pilT Acinetobacter nosocomialis M2

42.943

88.095

0.378
  pilT Acinetobacter baumannii strain A118

42.943

88.095

0.378
  pilT Legionella pneumophila strain Lp02

42.643

88.095

0.376
  pilT Legionella pneumophila strain ERS1305867

42.643

88.095

0.376
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.574

84.392

0.368
  pilT Vibrio cholerae strain A1552

43.574

84.392

0.368
  pilT Acinetobacter baylyi ADP1

42.073

86.772

0.365