Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PVV54_RS01480 Genome accession   NZ_CP118607
Coordinates   354486..355484 (+) Length   332 a.a.
NCBI ID   WP_274908263.1    Uniprot ID   -
Organism   Pseudomonas sp. PSKL.D1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349486..360484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVV54_RS01450 (PVV54_01450) - 349960..350394 (-) 435 WP_274908258.1 DUF4426 domain-containing protein -
  PVV54_RS01455 (PVV54_01455) metW 350417..351037 (-) 621 WP_110632944.1 methionine biosynthesis protein MetW -
  PVV54_RS01460 (PVV54_01460) - 351045..352184 (-) 1140 WP_274908259.1 homoserine O-acetyltransferase -
  PVV54_RS01465 (PVV54_01465) - 352273..352863 (-) 591 WP_274908260.1 YggT family protein -
  PVV54_RS01470 (PVV54_01470) proC 352874..353692 (-) 819 WP_274908261.1 pyrroline-5-carboxylate reductase -
  PVV54_RS01475 (PVV54_01475) - 353731..354417 (-) 687 WP_274908262.1 YggS family pyridoxal phosphate-dependent enzyme -
  PVV54_RS01480 (PVV54_01480) pilT 354486..355484 (+) 999 WP_274908263.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVV54_RS01485 (PVV54_01485) - 355551..356180 (-) 630 WP_274908264.1 C40 family peptidase -
  PVV54_RS01490 (PVV54_01490) - 356359..356778 (+) 420 WP_274908265.1 TM2 domain-containing protein -
  PVV54_RS01495 (PVV54_01495) - 356824..357522 (-) 699 WP_274908266.1 SPOR domain-containing protein -
  PVV54_RS01500 (PVV54_01500) argS 357524..359260 (-) 1737 WP_274908267.1 arginine--tRNA ligase -
  PVV54_RS01505 (PVV54_01505) - 359402..360397 (-) 996 WP_274907733.1 IS110 family transposase -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36169.96 Da        Isoelectric Point: 6.7011

>NTDB_id=793929 PVV54_RS01480 WP_274908263.1 354486..355484(+) (pilT) [Pseudomonas sp. PSKL.D1]
MDVTDLLARAVEAGASDLHLAAGEIPMLRLDGELRRMELPTLQAAELTDGMASLLDKPRQTQWLQGDELDLALELPTLGR
FRVNLFRQLRGPAVTLRLIPQRILTVEELGLEDVYRAVSQCADGLILVGGPTGSGKSSTLAALLDRLNRERPLHIITLED
PVEVIHSSHSSLVNHREVGRHCRDFSQGLRSALRQDPDVIMIGELRDLETIRLALRAAETGHLVLATVHTRSAAGSIDRL
VEVFAAEEKPLVRAMLAESLRLVVAQVLVKRIGGGRVAAREVLVATSAVRNLIREGRMAQLCSVMQSGAAEGMRTMEGAL
RGLKEKGLIGEL

Nucleotide


Download         Length: 999 bp        

>NTDB_id=793929 PVV54_RS01480 WP_274908263.1 354486..355484(+) (pilT) [Pseudomonas sp. PSKL.D1]
ATGGACGTGACCGACCTGTTGGCCCGAGCTGTGGAGGCAGGGGCAAGTGACCTGCATCTGGCTGCGGGCGAAATACCGAT
GCTGCGCCTGGATGGCGAGTTGCGGCGGATGGAATTGCCGACGTTGCAGGCTGCGGAGTTAACTGATGGCATGGCGTCTT
TGCTCGATAAGCCCCGGCAAACGCAATGGCTGCAGGGGGATGAGCTGGATTTGGCGCTTGAGCTGCCAACGCTTGGGCGC
TTTCGGGTAAATCTGTTTCGTCAGCTGAGGGGGCCGGCCGTGACTTTGCGTCTGATACCGCAGCGCATCCTGACGGTGGA
AGAACTCGGCCTGGAAGATGTGTATCGAGCTGTTTCGCAGTGTGCCGATGGCCTGATTCTGGTGGGTGGCCCGACCGGAA
GCGGTAAGTCCAGCACCTTGGCGGCGTTGCTCGACCGGCTGAACCGCGAGCGCCCACTGCACATCATCACCCTTGAAGAC
CCTGTGGAGGTTATCCACAGCAGCCACAGCAGCCTGGTGAATCATCGTGAGGTGGGCCGGCATTGCCGCGATTTTTCACA
GGGGCTACGTAGCGCCCTGCGTCAGGACCCGGACGTCATCATGATTGGCGAATTGCGTGACCTCGAAACCATTCGTTTGG
CCCTCAGAGCGGCGGAAACCGGGCATCTGGTGCTGGCGACGGTGCATACCCGTTCGGCGGCGGGCAGCATCGACCGGTTG
GTTGAGGTGTTTGCTGCCGAGGAAAAGCCACTGGTGCGGGCAATGCTGGCGGAGTCGCTGCGGTTGGTGGTGGCTCAGGT
GCTGGTCAAGCGGATTGGTGGTGGGCGGGTGGCTGCCCGGGAGGTGCTGGTGGCGACGTCAGCGGTGCGTAACCTGATTC
GCGAGGGGCGCATGGCACAGTTGTGTTCGGTGATGCAGAGCGGGGCGGCGGAGGGGATGCGGACGATGGAGGGCGCTTTG
CGGGGGCTGAAAGAAAAGGGGTTGATTGGCGAGTTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.799

99.096

0.533
  pilT Pseudomonas stutzeri DSM 10701

52.28

99.096

0.518
  pilT Acinetobacter baylyi ADP1

52.121

99.398

0.518
  pilT Acinetobacter nosocomialis M2

51.672

99.096

0.512
  pilT Acinetobacter baumannii D1279779

51.672

99.096

0.512
  pilT Acinetobacter baumannii strain A118

51.672

99.096

0.512
  pilT Legionella pneumophila strain ERS1305867

49.547

99.699

0.494
  pilT Legionella pneumophila strain Lp02

49.547

99.699

0.494
  pilT Neisseria meningitidis 8013

49.547

99.699

0.494
  pilT Neisseria gonorrhoeae MS11

49.245

99.699

0.491
  pilT Vibrio cholerae strain A1552

49.24

99.096

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

99.096

0.488
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.364

99.398

0.461
  pilU Pseudomonas stutzeri DSM 10701

36.364

99.398

0.361