Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PWW26_RS02940 Genome accession   NZ_CP118596
Coordinates   593443..594549 (+) Length   368 a.a.
NCBI ID   WP_274878530.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish10     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 588443..599549
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW26_RS02895 - 588561..588761 (+) 201 WP_005449722.1 hypothetical protein -
  PWW26_RS02900 - 588754..588909 (+) 156 WP_017190981.1 hypothetical protein -
  PWW26_RS02905 - 588979..589305 (+) 327 WP_017190980.1 hypothetical protein -
  PWW26_RS02910 - 589354..589605 (-) 252 WP_005449728.1 hypothetical protein -
  PWW26_RS02915 yggU 589781..590071 (-) 291 WP_005425805.1 DUF167 family protein YggU -
  PWW26_RS02920 - 590071..590628 (-) 558 WP_005425806.1 YggT family protein -
  PWW26_RS02925 proC 590682..591500 (-) 819 WP_038899445.1 pyrroline-5-carboxylate reductase -
  PWW26_RS02930 - 591632..592342 (-) 711 WP_009697582.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWW26_RS02935 pilT 592371..593411 (+) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  PWW26_RS02940 pilU 593443..594549 (+) 1107 WP_274878530.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWW26_RS02945 ruvX 594609..595034 (-) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  PWW26_RS02950 - 595084..595647 (-) 564 WP_005449740.1 YqgE/AlgH family protein -
  PWW26_RS02955 gshB 595778..596728 (-) 951 WP_274878531.1 glutathione synthase -
  PWW26_RS02960 rsmE 596743..597474 (-) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWW26_RS02965 endA 597621..598316 (-) 696 WP_005449743.1 deoxyribonuclease I -
  PWW26_RS02970 - 598468..598965 (-) 498 WP_009697583.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41291.43 Da        Isoelectric Point: 6.7731

>NTDB_id=793790 PWW26_RS02940 WP_274878530.1 593443..594549(+) (pilU) [Vibrio harveyi strain fish10]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMEPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=793790 PWW26_RS02940 WP_274878530.1 593443..594549(+) (pilU) [Vibrio harveyi strain fish10]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCGA
TGGAGCCGGAGCGACGACAAGAGTTTCGCAAAAGCCGCGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAACTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCGCTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTCGATCCAATGAATTTGGGATGTTGACCTTTGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.065

100

0.821
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Acinetobacter baylyi ADP1

41.916

90.761

0.38
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37