Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVT67_RS18090 Genome accession   NZ_CP118480
Coordinates   3677694..3678815 (+) Length   373 a.a.
NCBI ID   WP_301499733.1    Uniprot ID   -
Organism   Gallaecimonas kandeliae strain Q10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3672694..3683815
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT67_RS18065 (PVT67_18065) - 3673383..3674096 (+) 714 WP_336407778.1 glycosyltransferase family 2 protein -
  PVT67_RS18070 (PVT67_18070) - 3674096..3674362 (+) 267 WP_301496193.1 lipid-A-disaccharide synthase N-terminal domain-containing protein -
  PVT67_RS18075 (PVT67_18075) - 3674367..3676010 (+) 1644 WP_301496196.1 ArnT family glycosyltransferase -
  PVT67_RS18080 (PVT67_18080) - 3676098..3676829 (-) 732 WP_301496199.1 hypothetical protein -
  PVT67_RS18085 (PVT67_18085) - 3677158..3677634 (+) 477 WP_301496201.1 DUF4124 domain-containing protein -
  PVT67_RS18090 (PVT67_18090) pilU 3677694..3678815 (+) 1122 WP_301499733.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVT67_RS18095 (PVT67_18095) - 3678916..3680598 (+) 1683 WP_301496203.1 methyl-accepting chemotaxis protein -
  PVT67_RS18100 (PVT67_18100) - 3680588..3680911 (-) 324 WP_301496205.1 hypothetical protein -
  PVT67_RS18105 (PVT67_18105) - 3680960..3682396 (-) 1437 WP_301496221.1 guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase -
  PVT67_RS18110 (PVT67_18110) rhlB 3682407..3683711 (-) 1305 WP_301496223.1 ATP-dependent RNA helicase RhlB -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41451.48 Da        Isoelectric Point: 6.0224

>NTDB_id=792634 PVT67_RS18090 WP_301499733.1 3677694..3678815(+) (pilU) [Gallaecimonas kandeliae strain Q10]
MDIDQYLTVLARNEGSDLYLSTGAPPCAKFHGQLKALAKDALAPGEIAEVADFVMDEEQRLKFNKELEMNLARSIPGVGR
FRVNIFKQRNEVSIVARYIRNDIPKLEDLGMPEVLKDVIMSKRGLVLFVGATGSGKSTSLAALIDYRNSNASGHIITIED
PVEFVHKHKKSIINQREVGTDTRSFHAALINTLRQAPDVILIGEIRDRETMEHALAFAETGHLCISTLHANNANQALDRI
INFFPEERRPQLLLDLSLNLRAFVSQRLVPTVDGKRCAAVEILLGTSTVQDMVKRGDVHAIKEIMQKSENLGMQTFDTAL
FKLHMEGKIDLDQALRNADSVNDLRLKVKLAKNETAGSGAMDLRLEGEEGEDE

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=792634 PVT67_RS18090 WP_301499733.1 3677694..3678815(+) (pilU) [Gallaecimonas kandeliae strain Q10]
ATCGACATCGACCAATACCTGACAGTGCTGGCCCGCAACGAGGGCTCGGATCTCTACCTGTCCACAGGCGCGCCGCCCTG
CGCCAAGTTCCATGGCCAGCTCAAGGCCCTGGCCAAGGACGCCTTGGCCCCGGGTGAGATCGCCGAAGTGGCTGACTTCG
TCATGGACGAAGAGCAGCGACTCAAGTTCAACAAGGAGCTGGAGATGAACCTGGCCCGCTCCATTCCCGGTGTCGGTCGC
TTCCGGGTCAACATCTTCAAACAGCGCAACGAAGTCAGCATAGTGGCGCGCTATATCCGCAACGACATCCCCAAGCTGGA
AGACCTGGGCATGCCGGAAGTGCTCAAAGACGTGATCATGTCCAAGCGCGGCCTGGTGCTCTTCGTCGGTGCCACCGGTT
CGGGCAAGTCCACCTCCCTGGCGGCCCTCATCGACTACCGTAACAGCAACGCCAGCGGCCACATCATCACCATCGAAGAT
CCGGTGGAGTTCGTCCACAAGCACAAGAAGAGCATCATCAACCAGCGGGAAGTGGGCACAGACACCCGCAGTTTCCATGC
CGCCCTGATCAACACCCTGCGCCAGGCGCCTGACGTCATCCTCATCGGTGAGATCCGTGACCGCGAGACCATGGAGCACG
CCCTCGCTTTCGCCGAAACCGGCCACCTCTGTATCTCCACCTTGCACGCCAACAACGCCAACCAGGCCCTGGACCGTATC
ATCAACTTCTTTCCGGAAGAGCGCCGCCCCCAGCTGCTGTTGGACCTGTCCCTGAACCTGAGGGCCTTCGTCTCCCAACG
CCTGGTGCCGACGGTGGACGGCAAGCGCTGTGCCGCCGTGGAGATACTGCTGGGCACCTCCACAGTGCAGGACATGGTCA
AACGCGGCGATGTCCACGCCATCAAGGAGATCATGCAGAAGTCCGAGAACCTCGGCATGCAGACCTTCGACACAGCCCTG
TTCAAACTGCACATGGAAGGCAAGATCGACCTCGACCAGGCGCTGCGCAATGCCGACTCCGTCAACGACCTGCGCCTCAA
GGTCAAGCTGGCCAAGAACGAAACGGCCGGCAGCGGCGCCATGGACCTGCGCCTGGAAGGGGAAGAAGGCGAGGACGAAT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.425

95.979

0.542
  pilU Acinetobacter baylyi ADP1

51.412

94.906

0.488
  pilU Vibrio cholerae strain A1552

49.448

97.051

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.934

97.587

0.399
  pilT Pseudomonas stutzeri DSM 10701

42.308

90.617

0.383
  pilT Pseudomonas aeruginosa PAK

41.716

90.617

0.378
  pilT Acinetobacter baylyi ADP1

40.882

91.153

0.373
  pilT Neisseria gonorrhoeae MS11

40.762

91.421

0.373
  pilT Neisseria meningitidis 8013

40.762

91.421

0.373
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.818

88.472

0.37
  pilT Vibrio cholerae strain A1552

41.818

88.472

0.37
  pilT Acinetobacter baumannii strain A118

41.337

88.204

0.365
  pilT Acinetobacter baumannii D1279779

41.337

88.204

0.365
  pilT Acinetobacter nosocomialis M2

41.337

88.204

0.365