Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PotMrB4_RS05685 Genome accession   NZ_AP022642
Coordinates   1202881..1203363 (+) Length   160 a.a.
NCBI ID   WP_172432751.1    Uniprot ID   A0A679GMD8
Organism   Metapseudomonas otitidis strain MrB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197881..1208363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS05660 (PtoMrB4_11260) yacG 1198056..1198253 (-) 198 WP_044406857.1 DNA gyrase inhibitor YacG -
  PotMrB4_RS05665 (PtoMrB4_11270) coaE 1198250..1198861 (-) 612 WP_069559818.1 dephospho-CoA kinase -
  PotMrB4_RS05670 (PtoMrB4_11280) pilD 1198858..1199730 (-) 873 WP_172432748.1 A24 family peptidase Machinery gene
  PotMrB4_RS05675 (PtoMrB4_11290) pilC 1199733..1200950 (-) 1218 WP_172432749.1 type II secretion system F family protein Machinery gene
  PotMrB4_RS05680 (PtoMrB4_11300) pilB 1200954..1202657 (-) 1704 WP_172432750.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PotMrB4_RS05685 (PtoMrB4_11310) pilE 1202881..1203363 (+) 483 WP_172432751.1 pilin Machinery gene
  PotMrB4_RS05690 - 1203383..1205113 (+) 1731 WP_172432752.1 PglL family O-oligosaccharyltransferase -
  PotMrB4_RS05700 (PtoMrB4_11330) nadC 1205294..1206142 (-) 849 WP_172432753.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 160 a.a.        Molecular weight: 16043.41 Da        Isoelectric Point: 8.4765

>NTDB_id=79061 PotMrB4_RS05685 WP_172432751.1 1202881..1203363(+) (pilE) [Metapseudomonas otitidis strain MrB4]
MKAQKGFTLIELMIVVAIIGILAAVALPAYQDYTARAKVSEVVLAASSCRAAISEASQTGLASTPAANGFGCGETTSAVS
QYVASIQTTAAGAITVVAQNIAQVGTGKGITLTPYSDAAATTASVAADFTAGNLKPVRAWKCAGAATNPIEAKYLPASCR

Nucleotide


Download         Length: 483 bp        

>NTDB_id=79061 PotMrB4_RS05685 WP_172432751.1 1202881..1203363(+) (pilE) [Metapseudomonas otitidis strain MrB4]
ATGAAAGCTCAAAAAGGCTTTACCCTGATCGAATTGATGATCGTTGTTGCGATCATCGGCATTCTCGCCGCTGTTGCTCT
GCCTGCTTACCAGGACTACACCGCTCGCGCCAAGGTTTCTGAAGTGGTTCTGGCTGCTTCGTCCTGCCGTGCCGCTATCT
CTGAGGCTTCTCAGACCGGCCTCGCTTCTACTCCGGCCGCAAACGGCTTTGGCTGTGGTGAAACTACTTCTGCGGTTTCT
CAATACGTAGCGTCCATTCAAACTACTGCTGCCGGTGCTATCACCGTCGTCGCTCAGAATATCGCTCAGGTTGGTACTGG
TAAGGGCATCACTTTGACTCCCTATAGCGATGCCGCTGCTACCACCGCTTCTGTTGCTGCGGATTTCACTGCGGGTAATC
TGAAGCCGGTTCGCGCGTGGAAATGTGCTGGTGCTGCCACCAACCCGATCGAAGCCAAATATCTGCCGGCCAGCTGCCGC
TAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-160)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679GMD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

42.553

100

0.5
  pilA2 Legionella pneumophila str. Paris

45.912

99.375

0.456
  pilA2 Legionella pneumophila strain ERS1305867

45.283

99.375

0.45
  comP Acinetobacter baylyi ADP1

41.279

100

0.444
  pilA/pilA1 Eikenella corrodens VA1

40.361

100

0.419
  pilA Ralstonia pseudosolanacearum GMI1000

37.143

100

0.406
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.323

100

0.4
  pilE Neisseria gonorrhoeae strain FA1090

37.059

100

0.394
  pilE Neisseria gonorrhoeae MS11

34.659

100

0.381
  pilA Pseudomonas aeruginosa PAK

34.682

100

0.375
  pilA/pilAII Pseudomonas stutzeri DSM 10701

35.802

100

0.362

Multiple sequence alignment